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Citations for Evrogen :
201 - 250 of 381 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the PCR product and vector pTurboGFP-N (Evrogen) were digested with XhoI and HindIII purified and ligated to generate pGADD34-TurboGFP ...
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bioRxiv - Molecular Biology 2022Quote: ... The relative abundance of transcripts was estimated using quantitative real-time PCR with Hot Start DNA polymerase (Evrogen, Russia) and SYTO@ 13 green fluorescent nucleic acid stain (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA for qPCR was synthesized using random primers and the First Strand cDNA synthesis kit (MINT Reverse Transcriptase, Evrogen, Russia) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... TurboRFP (1:1000, Evrogen AB233), GFP (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 200 ng of plasmid pTagRFP-C (Evrogen), which expresses TagRFP from a CMV promoter ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR was performed in 15 μl reaction mixture of Screen Mix (Evrogen, Russia) in a Veriti Thermal Cycler (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... pHyPer-dMito (Evrogen, FP942), CystatinB C-GFPSpark (HG10435-ACG ...
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bioRxiv - Neuroscience 2022Quote: ... mTagBFP2 34 was generated from TagBFP (Evrogen). mNeonGreen 35 was obtained from Allele Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ERoxBFP was purchased from Addgene (68126) and pmKate2-paxillin from Evrogen (FP323). pEGFP-GRASP65 was a kind gift from Kasper Mygind at Institute Curie ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse anti-Aub antibody (1:20) (Patil and Kai 2010) or mouse anti-mKate2 (Evrogen, 1:200) was added to the cleared lysate and incubated at 4°C for 2 h with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... anti-tagRFP (1:1000; AB233, Evrogen), anti-Por1 serum (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... Rabbit anti-turboGFP (AB513) was purchased from Evrogen. Goat anti-GFP (GTX26673 ...
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bioRxiv - Developmental Biology 2022Quote: ... mKate2 was amplified from the pmKATE2-N plasmid from Evrogen introducing flanking B2 and BamHIXhoI-B3 sequences and cloned into pGEM-T Easy ...
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bioRxiv - Cell Biology 2022Quote: ... and in primary antibody (α-tRFP, [mKate antibody, Evrogen, AB233] ...
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bioRxiv - Cell Biology 2022Quote: ... EB1-TagRFP was generated by PCR amplification from KAZUSA cDNA (NCBI AB463888) and inserted into pTagRFP-N (Evrogen). The shRNA target sequence was designed for protein knockdown using the BLOCK-iT RNAi Designer tool (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... [48] fused with human IgG1 Fc-fragment and Llama IgG2b hinge were purchased from Evrogen (Russia). CHO-S cells were transfected with VHH-Fc containing construct using the CHO Gro System (Mirus Bio ...
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bioRxiv - Microbiology 2022Quote: ... DNA primer synthesis and DNA sequencing were performed by Evrogen (Russia). For the list of primers used in the study refer to Table S1 ...
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bioRxiv - Microbiology 2022Quote: ... and plasmid DNA was isolated using the Plasmid Miniprep/BC021S kit (Evrogen, Russia). The bla gene sequence and pblaTEM plasmid type were confirmed by PCR and Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 1× Duplex-Specific Nuclease (DSN) buffer (Evrogen), slow-ramped (3°C/min ...
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bioRxiv - Molecular Biology 2022Quote: ... following a duplex-specific nuclease (DSN, Evrogen) digestion ...
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bioRxiv - Systems Biology 2022Quote: ... qPCRmix-HS SYBR (Evrogen, Russia) containing HS Taq DNA polymerase ...
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bioRxiv - Neuroscience 2022Quote: ... pCAG-tRFP was generated by cloning the turboRFP (Evrogen) coding sequence into pCAG-GS ...
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bioRxiv - Plant Biology 2022Quote: cDNA was synthesized using the MMLV RT kit (Evrogen, Russian) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2022Quote: The KR from the plasmid vector – pKillerRed-mem (Evrogen, Moscow, Russia) was cloned with a human Rhodopsin promoter ...
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bioRxiv - Cell Biology 2022Quote: ... pTagRFP-C (#FP141) from Evrogen, fura-2-AM ...
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bioRxiv - Developmental Biology 2021Quote: ... using qPCRmix-HS SYBR master mix (Evrogen, Russia) with the following temperature program ...
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bioRxiv - Molecular Biology 2021Quote: We engineered the PB-TA-ERP2-mKate2-BRD4S construct from two addgene clones (6537854 and 8047755) and pmKate2-H2B (Evrogen, Russia). Overlap extension PCR was performed to amplify the mKate2 insert from the mKate2-H2B plasmid while adding the attB1 adapter and linker sequence ...
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bioRxiv - Molecular Biology 2021Quote: ... with a Screen Mix-HS polymerase kit (Evrogen). Amplifications were performed using a S1000™ thermal cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... gel-purified using the Cleanup Standard kit (Evrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... gel-purified using the Cleanup Standard kit (Evrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and kRas was amplified using the Encyclo GC polymerase kit (Evrogen; Russia), and control duplex 0Myc ...
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bioRxiv - Molecular Biology 2021Quote: Short double-stranded and partially single-stranded DNA templates containing the P077 promoter with uracils and thymines at certain positions were prepared by annealing of oligonucleotides ordered from Evrogen (Moscow) and listed in (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... or TagGFP2 (pTagGFP2-N vector, Evrogen) were used as controls for the crosstalk of the TagGFP2 signal into the red channel and the Katushka signal into the green channel ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mcg of total RNA was used for reverse transcription with MMLV-RT kit (Evrogen) according to the recommended protocol with oligo(dT)15 and random (dN)10 primers at a ratio of 1:3 ...
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bioRxiv - Molecular Biology 2021Quote: ... All amplification reactions were done with the Tersus Plus PCR kit (Evrogen).
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bioRxiv - Molecular Biology 2021Quote: ... 5μl 5x qPCRmix HS SYBR (Evrogen), forward primers corresponding to the beginning of transcripts (Msm-rpsOtr-for 5’-GTGGCTGTGTCGAGAATTTGTTCG for pS15Msm v.1 and 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... the resulting product was cleaned from 2% agarose gel by Cleanup Standard Kit (Evrogen), digested by MluI and HindIII and ligated into pMV306/MluI ...
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bioRxiv - Molecular Biology 2021Quote: ... smegmatis genomic DNA by using Tersus Plus PCR kit (Evrogen) with primers (−47 ...
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bioRxiv - Molecular Biology 2021Quote: A mouse codon-optimized version of the piggyBac transposase (PBase) was cloned in frame with the red fluorescent protein tagRFPt (Evrogen) into a pBroad3 vector (pBroad3_hyPBase_IRES_tagRFPt ...
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bioRxiv - Immunology 2021Quote: ... For quantitative PCR samples from adipose tissue were homogenized in ExtractRNA (Evrogen) which is Trizol analog ...
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bioRxiv - Immunology 2021Quote: ... Primers and probes were designed in NIH Primer BLAST and synthesis was performed by Evrogen (Moscow, Russian Federation).
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The amplified PCR product was digested with EcoRI and BamHI and then inserted into the pTagRFP-N1 vector (Evrogen, Moscow, Russia). After cloning into an expression vector ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... and cloned into a promoter-less vector encoding the red fluorescent protein TurboRFP (pTurboRFP-PRL; Evrogen, Moscow, Russia). U2OS cells were cotransfected with the EGFP-Tat ...
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Molecular coevolution of nuclear and nucleolar localization signals inside basic domain of HIV-1 TatbioRxiv - Evolutionary Biology 2021Quote: ... The membranes were blocked in 1% bovine serum albumin and incubated with either a monoclonal antibody against GFP (1:3000; Evrogen, Moscow, Russia) or a monoclonal antibody against B23 (1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... TagRFP (1: 1000, Evrogen, AB233), GFP (1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNA for transfection was isolated using Plasmid Miniprep Kit (Evrogen, Russia) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 5’-ccgggggcggccgctcaaagcttacttttgttcatatgtttattcaatgca-3’ (for pMF1992)) and subcloning the BamHI/NotI-restricted PCR products into the BamHI/NotI-restricted backbone fragments of pKillerRed-dMito (Evrogen) (for pMF1991 ...
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bioRxiv - Cell Biology 2021Quote: ... the mammalian expression vector pmKate2-mito (Evrogen plasmid #FP187) was used.
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bioRxiv - Cell Biology 2021Quote: ... the medium was replaced by live-cell visualization medium DMEMgfp-2 (Evrogen) supplemented with 10% FBS and 2 mM L- glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into Gateway® TagRFP-AS-N entry clone (Evrogen). The PRX62-TagRFP fusion was subcloned (Gateway Technology ...