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Citations for Lonza :
1 - 50 of 1631 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Transient expression of cDNAs or siRNAs was performed using the Amaxa nucleofector kit R (Lonza) and Amaxa Nucleofector II (Lonza ...
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bioRxiv - Immunology 2020Quote: ... a pMaxGFP expression construct (Lonza) was co-transfected with antibody expression constructs ...
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bioRxiv - Microbiology 2019Quote: ... All POLIC variant expression constructs (15 µg) were transfected by nucleofection using the Amaxa Nucleofection Parasite Kit (Lonza) into 29-13 cells to generate inducible overexpression POLIC-PTP variant cell lines (OE ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1 million cells were transfected with 2μg of donor vector and 2μg of each AAVS1 ZFN expression plasmids using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza). Cells were seeded in E8 medium supplemented with 10µM ROCK Inhibitor Y-27632 (Selleckchem) ...
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bioRxiv - Cell Biology 2022Quote: ... 2×105 NSCs for essential gene expression were nucleofected with 750 ng each of the above-mentioned plasmids using SG Cell Line 4DNucleofector X Kit (Lonza), DN-100 program ...
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bioRxiv - Bioengineering 2023Quote: Approximately 0.25 × 106 U2OS.eGFP-PEST cells were nucleofected with 300 ng of Cas9 expression plasmid and 30 ng of eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Bioengineering 2023Quote: ... and 50 ng of AcrIIA4 expression or 300 ng of Cas9-P2A-CSD-AcrIIIA4 plasmid and 30 ng eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and 200 ng pMaxGFP expression plasmid (Lonza, #13429329) via nucleofection (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... for all imaging experiments involving exogenous expression we used the Lonza Amaxa II Nucleofector System with Cell Line Nucleofector Kit V reagent (Lonza VCA-1003). Briefly ...
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bioRxiv - Genomics 2024Quote: ... and electroporated with 6 μg of gRNA-Cas9 expression vector and 3 μg of SNRPN targeting vector using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3032). Transfected cells were plated into a 10 cm dish coated with Matrigel (Corning ...
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bioRxiv - Cell Biology 2022Quote: ... within 24hrs of eclosion were fed wet yeast paste for 72 hours at room temperature before dissection in room-temperature Grace’s medium (Lonza). Ovaries were fixed in 4% paraformaldehyde in Grace’s medium for 10 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1 μg of sgRNA-CAS9 expression vector by 4D-Nucleofector (Lonza) using P3 Primary Cell 4D-Nucleofector X kit (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
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bioRxiv - Immunology 2023Quote: ... PmaxTM GFP expression vector was acquired from Lonza (cat numb #D-00061). Expression plasmid for ZIKV NS5 was generated by amplifying the NS5 coding sequence (amino acids 2521-3423 in the polyprotein ...
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bioRxiv - Immunology 2020Quote: ... Nucleotide sequences were synthesized by GeneArt and subcloned into expression vector pEE6.4 (Lonza) for expression.
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bioRxiv - Biochemistry 2020Quote: ... Resulting viruses were used to infect Sf21 expression cultures in insect-Xpress media (Lonza). Expression culture cells were harvested 48 hours after proliferation arrest ...
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bioRxiv - Bioengineering 2022Quote: All coding sequences used in this study were cloned into pmax expression vector (Lonza) by TEDA as previously reported (46) ...
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bioRxiv - Microbiology 2019Quote: ... BACs together with pp71 expression plasmid were transfected into 3T3 fibroblasts using an Amaxa nucleofector (Lonza).
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bioRxiv - Developmental Biology 2019Quote: ... The human Atoh8 expression plasmid [30] was transfected by electropulsing using the Cell Line Nucleofector System (Lonza) following standard procedures provided by the manufacturers.
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bioRxiv - Neuroscience 2022Quote: ... GFP expression plasmid was inserted into CAP-exosomes using 4-D-Nucleofector (instruments and reagents from Lonza, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... pX459-derived expression plasmids were transfected into MDCK cells by an Amaxa Nucleofector II (Lonza, Basel, Switzerland). The transfected cells were selected with 2.0 µg mL-1 puromycin ...
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bioRxiv - Immunology 2022Quote: ... and protein expression was carried out in suspension Trichoplusiani ni (High Five) cells grown in InsectXpress media (Lonza).
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bioRxiv - Cell Biology 2022Quote: ... expression constructs were transfected into cells using the Amaxa™ 4D-Nucleofector (10μg plasmid cDNA; Lonza, Basel, Switzerland) or JetPrime (2μg plasmid cDNA ...
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bioRxiv - Developmental Biology 2019Quote: ... U2OS cells were transfected with Cas9 and guide RNA expression plasmids using Amaxa electroporation as recommended by the manufacturer (Lonza). Cas9 expression plasmid was from the Church lab (Addgene 41815) ...
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bioRxiv - Biophysics 2021Quote: ... 2 μg of each Pcdh expression construct were transfected into 20 μL of the K562 cell suspension by electroporation using an Amaxa 4D- Nucleofector (Lonza). Transfected cells were transferred to a 24-well plate in 500 μL of medium per well and incubated overnight at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... expressing the human CD133 antigen45 was co-delivered with a PBase expression vector (2.5 μg) into 2 ⨯ 106 U251 cells using Nucleofector 2b (Lonza, Köln, Germany), followed by puromycin (500 ng/ml ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Genomics 2021Quote: ... mESCs in 6-cm dishes were transfected with 7.2 μg pX330-Cas9 plus 1.8 μg GFP expression vector by 4D-nucleofector X (Lonza, solution Cytomix, program GC104), then harvested for genomic DNA 3 days after transfection.
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bioRxiv - Genomics 2021Quote: ... Growing CH12F3 cells were transfected with 1.5 μg pX330-Cas9 or pX330-Cas9-mCherry expression vector per million by 4D-nucleofector X (Lonza, solution M1, procedure DN100) and seeding at 0.5 million cells/mL in fresh medium with 1μg/mL anti-CD40 ...
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bioRxiv - Molecular Biology 2021Quote: ... All sgRNA encoding oligonucleotides were inserted into a retroviral U6-sgRNA-PGK-GFP expression vector61 using a BsmBI restriction site and transfected into cells by Amaxa II electroporator (Lonza; program G-016) and Amax II Cell Line Nucleofector Kit (R ...
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bioRxiv - Biochemistry 2022Quote: All proteins were expressed using the baculoviral expression system and the lepidopteran High Five cell line in Insect-XPRESS (Lonza, cat. no. BELN12-730Q). Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences) ...
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bioRxiv - Bioengineering 2021Quote: ... 1.0×106 K562 cells harboring a monoclonal Kp03 landing pad and multiclonal rTetR-VP48 expression construct were electroporated in 100 μL Amaxa solution (Lonza Nucleofector 2b, program T-016) with 1000 ng of reporter donor plasmid (pMMH56,59,59) ...
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bioRxiv - Molecular Biology 2019Quote: ... buffer kit V (Lonza) and program A-023 ...
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bioRxiv - Cancer Biology 2021Quote: Mycoplasma Detection Kits (Lonza) and MycoSensor qPCR Assay Kits (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... (kit C, W001; Lonza) and CD81 positive cells sorted (ARIA III BD ...
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bioRxiv - Immunology 2019Quote: The Nucleofector transfection kit (Lonza) was used to transfect siRNA into cells ...
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bioRxiv - Microbiology 2023Quote: A ToxiLight BioAssay Kit (Lonza) was used to determine cell death ...
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bioRxiv - Microbiology 2023Quote: ... and Nucleofector Kit R (Lonza) following manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... using ToxiLight bioassay kit (Lonza) according to the manufacture’s recommendations.
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bioRxiv - Biophysics 2019Quote: ... These growth factors were supplied as a bullet kit (Cell Media and Bullet Kit, Lonza). HUVECs were passaged 2-6 times.
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bioRxiv - Immunology 2020Quote: ... The MycoAlert Mycoplasma Detection Kit (Lonza) was used to monitor mycoplasma contamination on a regular basis.
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bioRxiv - Bioengineering 2019Quote: ... and EGM-2 bullet kit (Lonza)) and maintained at ALI for three weeks.
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bioRxiv - Developmental Biology 2019Quote: ... using a HUVEC Nucleofector kit (Lonza) according to manufacturer’s instructions and the cells were further cultured for 72 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... using the MycoAlert Kit (Lonza, Switzerland). NIH3T3 mouse embryonic fibroblasts were stably transfected with pCMV_HER4 that encodes the JMa/CYT1 isoform ...
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bioRxiv - Immunology 2021Quote: ... and Nucleofector kits (Lonza, VPB-1002) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... MycoAlert Mycoplasma Detection Kit (Lonza, Bioscience). Each cell lines were used within passage 4/5 since their thawing from originally frozen vials.
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bioRxiv - Cancer Biology 2022Quote: ... Mycoplasma testing (MycoAlert Detection Kit, Lonza) was performed monthly.
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bioRxiv - Cell Biology 2022Quote: ... using MycoAlert detection kit (Lonza, LT07). Additionally ...
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bioRxiv - Cancer Biology 2022Quote: ... Regular testing with MycoAlert kit (Lonza) confirmed the absence of mycoplasma contamination ...
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bioRxiv - Cancer Biology 2020Quote: ... using MycoAlert™ kit (Lonza, Germany) and genetically authenticated using a STR-PCR fragments kit (Agilent Technologies ...