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Citations for Lonza :
1 - 50 of 1831 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cells were confirmed mycoplasma-free every two months via MycoAlert Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely (every two months) screened for Mycoplasma contamination by MycoAlert Kit (Cat#LT07218, Lonza) as the manufacturer’s introductions ...
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bioRxiv - Genetics 2021Quote: ... Two million WTC iPSC were nucleofected using an Amaxa nucleofector 2B and the Nucleofector Kit C (both from Lonza), 0.5 µg of each AAVS1 TALEN pair and 1 µg of the donor plasmid (see Figure S4) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were screened every two months for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza, Basel, Switzerland). Passaged cells were continually monitored for viability using Countess cell counting slides (Thermo-Fisher ...
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bioRxiv - Bioengineering 2023Quote: Ha deposition was detected staining constructs sections (n≥10 from n≥3 chambers considering two different hACs and two different MSCs donors) with OsteoImage (Lonza) as detailed above ...
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bioRxiv - Immunology 2021Quote: ... WT RAW264.7 cells were subjected to two rounds of nucleofection with Cas9-RNPs using the SF Cell Line 4D-Nucleofector X Kit S (Lonza) and the 4D Nucleofector apparatus precisely as specified by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... cellular ATP content was measured two-three times a week between 5-7.5 weeks post transduction using the ViaLight Plus kit (Lonza, LT07-221) or ATPlite kit (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: ... conjugated to FITC or GFP siRNA (300 nM) conjugated to FITC two days prior to adoptive T cell therapy using the Mouse T cell Nucleofector™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: Generation of Foxa2 knockout cell lines was performed by electroporating two different guides cloned into the pX458 vector (Ran et al., 2013) with the AMAXA nucleofector kit (Lonza Cat no. VPH-1001). Cells were sorted for GFP as single cells one day after electroporation ...
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bioRxiv - Cancer Biology 2021Quote: ... Two hundred microliters of human microvascular endothelial cells suspension (Lonza, Basel) (6.25×105 cells/ml ...
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bioRxiv - Cell Biology 2022Quote: ... Two IPF and one control cell isolate was purchased from Lonza (catalog number CC7231 and CC2512 ...
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bioRxiv - Genomics 2023Quote: ... we transfected two million mESCs by electroporation using Amaxa nucleofector (Lonza) or Neon kits ...
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bioRxiv - Molecular Biology 2019Quote: ... was modified by adding T2A-GFP at the C-terminal end and electroporated with the sgRNA vector into JB1 and BJ1 hybrid ES cells using the Amaxa nucleofector procedure (Lonza). 24 h post-electroporation ...
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bioRxiv - Molecular Biology 2022Quote: ... Two-dimensional gel electrophoresis was performed in 0.4% SeaKem Gold agarose (Lonza) and 1X TBE in the first dimension ...
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bioRxiv - Neuroscience 2022Quote: ... transfection system with Primary Cell Optimization 4D-Nucleofector X Kit (Cat: #V4XP-9096, Lonza) in P2 buffer – program DS-150.
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... the OMC was conditioned for two days with X-VIVO-15 medium (Lonza) supplemented with 5% human serum (Sanquin) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were collected after two days in SKGM (SKGM-2, Lonza CC-3245) culture ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected by electroporation using the Amaxa Nucleofector system Kit V (Lonza: VCA-1003). The following day ...
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bioRxiv - Developmental Biology 2023Quote: ... Two to three million NSCs were resuspended in 100 μL P3 primary solution (Lonza) containing 10 μg of the plasmid DNA and transfected using the program DS-112 of the 4D-nucleofector (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... and RBC lysis was performed for two minutes (ACK lysing buffer, Lonza, Basel, Switzerland). Following a wash step ...
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bioRxiv - Cancer Biology 2019Quote: ... The above two plasmids were co-transfected into HCT-116 cells using 4D Nucleofector (Lonza). The puromycin-resistant clones were screened by genomic PCR and sequencing.
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bioRxiv - Molecular Biology 2023Quote: A Lonza Nucleofector system including a Nucleofector 2b Device and the Cell Line Nucleofector Kit V (Lonza) were used for electroporation of LbuCas13a protein and guide RNA ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were regularly tested for Mycoplasma contamination using a commercially available system (MycoAlert Mycoplasma Detection kit, Lonza).
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bioRxiv - Molecular Biology 2023Quote: ... using the CRISPR/Cas9 system and the P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, Switzerland). Oligonucleotides for gRNA were selected by Сrispor (Tefor Infrastructure ...
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bioRxiv - Bioengineering 2021Quote: ... ii Quantification of monolayer hydraulic conductivity for two cell lines (Lonza, light blue; ScienCell, dark blue) and for two dextran molecular weights (MW) ...
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bioRxiv - Pathology 2023Quote: ... Cells were stimulated with anti-CD3/CD28 beads (MiltenyiBiotec) for two days in cRF10: RPMI (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... cell pellets were resuspended in two to three volumes of ACK lysis buffer (Lonza: #10-548E) according to the manufacturers’ specifications ...
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bioRxiv - Microbiology 2020Quote: Electroporation was performed using the Amaxa P3 Primary Cell 96-well Nucleofector kit and 4D Nucleofector system (Lonza). Recombinant S ...
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Biochemistry 2022Quote: Human Aortic Endothelial Cells (HAEC) derived from two the same age male donors were purchased from Lonza. Cells were grown in Endothelial Cell Growth Medium BulletKit®-2 (EGM-2 BulletKit ...
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bioRxiv - Systems Biology 2021Quote: ... and two layers were added into each well [1X Eagle Minimum Essential Medium (EMEM; 12-684, Lonza); 10% FCS ...
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bioRxiv - Synthetic Biology 2023Quote: PBMCs were thawed two days before transduction and maintained in X-Vivo 15 media (Lonza 04-418Q) supplemented with 5% human AB serum (Valley Biomedical HP1022) ...
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bioRxiv - Immunology 2023Quote: ... and the two lobes were separated and embedded in 4% low melting point agarose (Lonza, NJ, USA). 400µm thymic slices were generated by slicing the trimmed agarose blocks on a VT 1000S vibratome (Leica ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were pulsed with protocol EO115 using the P3 Primary Cell 4D-Nucleofector Kit and 4D-Nucleofector System (Lonza). Cells were recovered immediately with warm media for 6 hours before transduction with CAR ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were confirmed mycoplasma-negative every two weeks and were last tested January 2019 (Lonza, # LT07-703). To maintain cellular phenotypes and to prevent cross-contamination of murine cell lines ...
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bioRxiv - Cell Biology 2023Quote: ... Two days after transfection of WT HeLa cells with pSpCas9(BB)-2A-GFP-ghMyo10 using Amaxa nucleofection (Lonza), GFP-positive cells were subjected to single-cell sorting into 96-well plates using a BD FACS cell sorter ...
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bioRxiv - Systems Biology 2021Quote: ... and 2×106 iPSCs were mixed with pX459-sgRNA vector and ssDNA repair donor template and electroporated with Lonza 4D-Nucleofector system using the P3 Primary Cell Nucleofector Kit (Lonza). The iPSCs were then recovered in mTeSR1 medium for 48 hours before adding puromycin for 3-day selection ...
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bioRxiv - Neuroscience 2020Quote: iPSNs were transfected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza). POM121 ...
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bioRxiv - Immunology 2021Quote: ... MICB or ULBP-1 genes after optimizing nucleofection conditions using primary cell 4D nucleofector kit and 4D nucleofector system (Lonza). After 48 hours of culture ...
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bioRxiv - Microbiology 2022Quote: ... All cell lines were regularly tested to check they were free of mycoplasma contamination using a commercially available system (MycoAlert Mycoplasma Detection kit; Lonza). HCV replication experiments were conducted using the highly permissive human hepatoma cell line Huh7 High Passage (28 ...
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bioRxiv - Cell Biology 2022Quote: ... we used Cas9-RNP and the Lonza 4D-Nucleofector System together with the P4 Primary Cell 4D-Nucleofector X Kit L (Lonza) to transfect and create Ndrg2-KO DCs ...
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bioRxiv - Cell Biology 2022Quote: ... For nucleofection we used the 4D-Nucleofector System and P3 Primary Cell 4D Nucleofector X Kit L (Lonza, Basel, Switzerland). We optimized the RNP electroporation for DCs by using multiple-guide mixtures and different number of cells in a 100uL total volume ...
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bioRxiv - Neuroscience 2020Quote: ... control and C9orf72 iPSNs were nucleofected using the Lonza nucleofection system (program DC104) and a Lonza P3 Primary Cell 4D Nucleofector Kit (Lonza) as described above ...
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bioRxiv - Genetics 2022Quote: ... Libraries were electroporated into K562 cells in 100ul volumes using the Amaxa system (Program X-001, Neucleofactor kit V, Lonza). We performed 6 independent replicates with each replicate consisting of ∼2×108 cells ...
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bioRxiv - Developmental Biology 2020Quote: ... The guide RNA vector was then electroporated into the parent line containing the CRISPRi system using the Human Stem Cell Nucleofector Kit 1 solution with the Amaxa nucleofector 2b device (Lonza). Nucleofected cells were then seeded into a 6-well plate in mTeSR™-1 supplemented with Y-27632 (10 μM ...
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bioRxiv - Immunology 2020Quote: ... Activated cells were transfected using the Human T cell Nucleofector Kit (Amaxa Biosystem, #VPA-1002) and the Amaxa Nucleofector II system (Lonza), Program T-023 with ribonucleoprotein complexes ...
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bioRxiv - Neuroscience 2023Quote: Transfection of dissociated neurons was performed using the AMAXA Nucleofector system (program O.005) with the mouse neuron Nucleofector kit (Lonza) following the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: Transfections of K562 cells were performed using a Lonza Bioscience 4D-Nucleofector system and the SF Cell Line 4D-Nucleofector X kits (Lonza). For single nucleocuvettes (100 uL) ...
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bioRxiv - Cell Biology 2023Quote: ... nucleofected in a 100 µl reaction using the Lonza 4D-Nucleofector System with P3 Primary Cell 4D-Nucleofector X Kit (Lonza) and program CA137 ...