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Citations for Lonza :
1 - 38 of 38 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: CHO cells stably expressing recombinant RBD of SARS-CoV-2 Spike (aa. 319-591) (GenBank accession no. AAP13567.1) were propagated in ProCho5 media (Lonza) containing glutamine ...
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bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
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bioRxiv - Immunology 2020Quote: HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were grown in DMEM medium (Lonza) with 10% fetal bovine serum (Biowest ...
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bioRxiv - Biophysics 2021Quote: ... containing AMUPol and electroporated (HEK293 pulse sequence, Lonza 4D-nucleofactor) using manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... containing AMUPol and electroporated (HEK293 pulse sequence, Lonza 4D-nucleofactor) using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cell line was grown in DMEM medium (Lonza™ BioWhittaker™) supplemented with 10% of heat-inactivated fetal bovine serum (Biowest™ ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells (ATCC CRL-1573) were cultured in DMEM (Lonza BE12–604F) supplemented with 10% fetal calf serum (GE Healthcare ...
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bioRxiv - Systems Biology 2022Quote: ... BJ5ta cells and HEK293 cells were transfected using the 4D nucleofector (Lonza) and Neon transfection system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: HeLa and HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were grown in DMEM containing high glucose and sodium pyruvate (Lonza) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Systems Biology 2023Quote: ... SARS-CoV-2 was mycoplasma negative (Lonza MycoAlert Mycoplasma Detection Kit). All experiments in this study that utilized cultured SARS-CoV-2 were conducted in a biosafety-level 3 laboratory.
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bioRxiv - Genomics 2023Quote: ... 0.5 µg of these constructs were nucleofected into HEK293 cells using 4D Nucleofector (Lonza) and luciferase activity was measured 24 h post transfection using Spectramax plate reader (Molecular Devices) ...
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bioRxiv - Neuroscience 2022Quote: HEK293 Sham and HEK293_GLT8D1-WT/R92C stable cell lines were cultured in 10mL DMEM (Lonza) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK293 (human embryonic kidney 293) cells were grown in Dulbeccos Modified Eagles Medium (DMEM, Lonza, Basel, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... 1·106 of HEK293 cells were resuspended in 100 μL of pre-warmed Nucleofector solution V (Lonza). The plasmid containing c17orf80 gRNA and Cas9 (4 μg ...
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bioRxiv - Immunology 2022Quote: ... Co-cultures were done in IMDM supplemented with 5% HI-HS (Lonza) and gentamycin (86 μg/mL ...
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bioRxiv - Bioengineering 2019Quote: Cell culture: Glut1 experiments were done on HEK293 (ATCC #CRL-1573) cells transfected using the Amaxa system (Lonza) and 1 µg DNA ...
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bioRxiv - Microbiology 2019Quote: ... and 25 ng/ml of amphotericin B. HEK293 cells (obtained from M. Marketon’s laboratory) were maintained in Dulbecco’s modified essential medium (DMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: HeLa cells and HEK293 cells obtained from Melanie Marketon’s lab at Indiana University were maintained in Dulbecco’s MEM (Lonza) supplemented to 5% FBS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells were passaged in poly-D-Lysine treated plates and incubated overnight in OPTI-MEM medium (Lonza) at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Human embryonic kidney 293 (HEK293) cells (ATCC; CRL-1573) were grown in high-glucose DMEM (Lonza; BE12-604F) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...
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bioRxiv - Neuroscience 2023Quote: ... dissociated neurons were electroporated with cDNA (2 µg) or siRNA (Table S1) using an Amaxa nucleofector (program-005, Lonza) and mouse neuron nucleofectorTM kit (VPG-1001 ...
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bioRxiv - Biochemistry 2023Quote: Plasma cholesterol was depleted by treating HEK293 cells with 5 mM MβCD for 30 min in Pro293A-CDM (Lonza); this short period of MβCD treatment was deemed sufficient to removed 50% of endogenous cholesterol from cells (34) ...
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bioRxiv - Bioengineering 2022Quote: ... and NP-C1 patient fibroblasts (GM18393 and GM17919A) were purchased from the Coriell Cell Repository (Table S1) and cultured in DMEM (Lonza) supplemented with 20% FBS (Bovogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were washed twice with PBS and 2 × 106 cells were resuspended in 100 μl of Solution V (Lonza) and then mixed with 5 μg purified sgRNA expression vector or the empty vector as a negative control before nucleofection using program Q-001 of a Nucleofector™ 2b (Lonza) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells (used for TT-TL seq and ChIP-seq spike-ins) were cultured in Schneider’s modified Drosophila medium (Lonza) supplemented with 10% FBS at 27°C without CO2 ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of sgRNA and 5 µg of ssDNA donor template (Table S1) using Human Stem Cell NucleofectorTM kit 1 (Lonza, VVPH-5012) according to the manufactures protocol and cells replated ...
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bioRxiv - Neuroscience 2019Quote: ... Co-transfection of 500 ng sgRNA plasmid and 1 µl of 10 µM ssODN template into 2×105 HEK293 cells was performed using nucleofection on the 4D-Nucleofector system (LONZA, Basel, Switzerland), as described previously38 ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids expressing ETS1 sgRNAs (see Supplemental Table S1) were co-electroporated into the dCas9-VP64-expressing cells using the AmaxaTM Cell Line Nucleofector™ Kit R (Lonza, VCA-1001) and further subjected to puromycin selection and single-cell cloning ...
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bioRxiv - Microbiology 2020Quote: ... and 3-fold (SARS-CoV-2 and SARS-CoV) serial dilutions of sample in serum-free EMEM supplemented with 20 µg/ml trypsin (Lonza) for MDCK cells ...
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bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
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bioRxiv - Cell Biology 2024Quote: Human primary lung microvascular endothelial cells (LMVECs) and pulmonary artery endothelial cells (PAECs) from different donors were purchased from Lonza (Walkersville, MD. See Table S1). Cells were seeded onto plates and cultured in complete medium comprising of endothelial basal medium-2 (EBM-2 ...
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bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
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bioRxiv - Cancer Biology 2023Quote: Cell and organoid lines were generated from C3-TAg tumors (supplementary file 1) and tested for mycoplasma (Lonza, LT07-703) prior to use and the creation of frozen stocks ...