Labshake search
Citations for Lonza :
1 - 50 of 975 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: CHO cells stably expressing recombinant RBD of SARS-CoV-2 Spike (aa. 319-591) (GenBank accession no. AAP13567.1) were propagated in ProCho5 media (Lonza) containing glutamine ...
-
bioRxiv - Microbiology 2021Quote: Purified SARS-CoV-2 RBD and SED (Spike ectodomain) were diluted in DPBS (without Ca and Mg, Lonza, 17-512F) and coated onto 96-well plates (MaxiSorp - ThermoFisher or High binding - Greiner Bio-one ...
-
bioRxiv - Systems Biology 2023Quote: ... SARS-CoV-2 was mycoplasma negative (Lonza MycoAlert Mycoplasma Detection Kit). All experiments in this study that utilized cultured SARS-CoV-2 were conducted in a biosafety-level 3 laboratory.
-
bioRxiv - Microbiology 2020Quote: ... and 3-fold (SARS-CoV-2 and SARS-CoV) serial dilutions of sample in serum-free EMEM supplemented with 20 µg/ml trypsin (Lonza) for MDCK cells ...
-
bioRxiv - Bioengineering 2023Quote: ... 2×109 Freestyle CHO-S cells were resuspended in 500ml ProCHO 4 Protein-free Medium (Lonza, Cat#BEBP12-029 ...
-
bioRxiv - Immunology 2024Quote: ... rMASPs were expressed in CHO-DG44 cells cultivated in Power CHO serum-free media (Lonza, Vallensbaek, Denmark) supplemented with 20 U/mL penicillin ...
-
bioRxiv - Microbiology 2021Quote: ... in Pro-CHO-5 media (Lonza, Switzerland).
-
bioRxiv - Immunology 2023Quote: ... CHO DG44 cells were cultured in ProCHO5 (Lonza) supplemented with 4 mM L-glutamine ...
-
bioRxiv - Bioengineering 2022Quote: ... CHO-mAb cell line was maintained in PowerCHO2CD medium (Lonza) supplemented with glutamine synthetase expression medium (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... dissociated neurons were electroporated with cDNA (2 µg) or siRNA (Table S1) using an Amaxa nucleofector (program-005, Lonza) and mouse neuron nucleofectorTM kit (VPG-1001 ...
-
bioRxiv - Immunology 2021Quote: ... The IFNα-RBD sequence was then cloned into the PEE12.4 (Lonza) with a human IgG1 Fc ...
-
bioRxiv - Cell Biology 2023Quote: ... with a blue fluorescent protein (BFP)-tag were cultured in smooth muscle cell growth medium-2 (SMGM2) (Lonza). Human umbilical cord vein endothelial cells (HUVEC ...
-
bioRxiv - Microbiology 2022Quote: ... 1.2E6 BHK21 cells were nucleofected with 2 µg of Spike plasmid using an Amaxa Nucleofector II with cell line kit L (Lonza #VCA-1005) and program A-031 ...
-
bioRxiv - Immunology 2022Quote: The pXC17.4-RBD plasmid was transfected into CHOK1SV GS-KO cells (Lonza) by electroporation and stably transfected clones were selected by addition of methionine sulphoxamine (Sigma Aldrich ...
-
bioRxiv - Biophysics 2020Quote: CHO K1 cells were grown at 37 °C at 5% CO2 in DMEM/F12 (Lonza) supplemented with 10 % FBS and L-Glutamine (both Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... suspension CHO cells expressing wildtype human CTLA-4 were plated at 1 × 106 cells/well in DPBS (Lonza, Basel, Switzerland) containing 0.5% BSA (MilliporeSigma ...
-
bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
-
bioRxiv - Immunology 2022Quote: ... Co-cultures were done in IMDM supplemented with 5% HI-HS (Lonza) and gentamycin (86 μg/mL ...
-
bioRxiv - Cell Biology 2022Quote: Normal human bronchial epithelial cells (HBECs) from 3 independent donors (Supplemental Table S1) were obtained from Lonza (CC-2540) and cultured in BEGM growth media (Lonza CC-3170) ...
-
bioRxiv - Bioengineering 2022Quote: ... and NP-C1 patient fibroblasts (GM18393 and GM17919A) were purchased from the Coriell Cell Repository (Table S1) and cultured in DMEM (Lonza) supplemented with 20% FBS (Bovogen) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells (used for TT-TL seq and ChIP-seq spike-ins) were cultured in Schneider’s modified Drosophila medium (Lonza) supplemented with 10% FBS at 27°C without CO2 ...
-
bioRxiv - Biochemistry 2022Quote: ... Monoclonal cells were grown in serum-free CHO medium (Bio&SELL, Feucht, Germany) or Excell medium supplemented with 50 μg/mL gentamicin sulphate (Lonza™ BioWhittaker™, Walkersville, MD, USA) at 37°C and 5% CO2.
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg of sgRNA and 5 µg of ssDNA donor template (Table S1) using Human Stem Cell NucleofectorTM kit 1 (Lonza, VVPH-5012) according to the manufactures protocol and cells replated ...
-
bioRxiv - Cancer Biology 2023Quote: ... Plasmids expressing ETS1 sgRNAs (see Supplemental Table S1) were co-electroporated into the dCas9-VP64-expressing cells using the AmaxaTM Cell Line Nucleofector™ Kit R (Lonza, VCA-1001) and further subjected to puromycin selection and single-cell cloning ...
-
bioRxiv - Cell Biology 2024Quote: Human primary lung microvascular endothelial cells (LMVECs) and pulmonary artery endothelial cells (PAECs) from different donors were purchased from Lonza (Walkersville, MD. See Table S1). Cells were seeded onto plates and cultured in complete medium comprising of endothelial basal medium-2 (EBM-2 ...
-
bioRxiv - Microbiology 2022Quote: ... 2 ml of 2% SeaPlaque™ Agarose (Lonza) in DMEM containing 2% FBS and 1% penicillin/streptomycin (P/S ...
-
bioRxiv - Microbiology 2021Quote: ... 2◻ml of 2% Seaplaque agar (Lonza) in Dulbecco’s modified Eagle medium (DMEM ...
-
bioRxiv - Microbiology 2021Quote: ... 2◻ml of 2% Seaplaque agar (Lonza) in DMEM containing 2% FBS ...
-
bioRxiv - Bioengineering 2020Quote: ... HUVECs were cultured in Endothelial Growth Medium 2 (EGM-2) and NHLFs were cultured in Fibroblast Growth Medium 2 (FGM-2) (Lonza, Walkersville, MD). All cells were cultured in incubators at 37 °C and 5% CO2 ...
-
bioRxiv - Bioengineering 2021Quote: ... were purchased from Lonza and cultured in EGM-2 and FGM-2 (Lonza) supplemented with EGM-MV and FGM-2 Bullet Kit ...
-
bioRxiv - Bioengineering 2022Quote: ... Endothelial Cell Growth Medium-2 (EGM-2, Lonza, Switzerland) was used as the medium ...
-
bioRxiv - Cancer Biology 2023Quote: Cell and organoid lines were generated from C3-TAg tumors (supplementary file 1) and tested for mycoplasma (Lonza, LT07-703) prior to use and the creation of frozen stocks ...
-
bioRxiv - Bioengineering 2022Quote: Endothelial cords were cultured in EGM-2 (EBM-2 with the addition of EGM-2 BulletKit) (Lonza). For most experiments ...
-
Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ... were cultured in endothelial basal medium-2 (EBM-2) supplemented with EGM™-2 BulletKits™ (Lonza). Cells at passage 3 were used.
-
bioRxiv - Physiology 2020Quote: ... were cultured in endothelial basal medium-2 (EBM-2) supplemented with EGM™-2 BulletKit™ (Lonza). Cell from passage 3 to passage 6 were used ...
-
bioRxiv - Bioengineering 2020Quote: ... and 40 % EGM-2 (EBM-2 Lonza CC-3156; Clonetics) formed the basic medium ...
-
bioRxiv - Bioengineering 2022Quote: ... were obtained from Lonza and cultured in Endothelial Growth Medium-2 (EGM-2, Lonza) or EGM-2 BulletKit (Lonza) ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured in EBM-2/EGM-2 medium (Lonza) with 12% FBS ...
-
bioRxiv - Immunology 2020Quote: ... coated plates in Keratinocyte Growth Medium 2 (KGM-2, Lonza) at a density of 2 x 105 cells/cm2 and cultured at 37°C at 5% CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... They were cultured in EC specific medium with 2% FBS (EGMTM-2 EC Growth Medium-2 BulletKitTM, LONZA® or EC Growth Medium 2, PromoCell®) at 105 cell/well in a 6 well plate ...
-
bioRxiv - Cell Biology 2020Quote: ... EBM-2 (Endothelial Cell Growth Basal Medium-2, Lonza, #CC-3156) was supplemented with EGM-2 SingleQuots Supplements (Lonza ...
-
bioRxiv - Bioengineering 2022Quote: ... were purchased from Lonza and cultured in endothelial growth medium 2 (EGM-2) medium (Lonza). HUVECs were cultured in T-75 flasks in a humidified cell culture incubator at 37 °C and 5 % CO2 with culture medium being changed every two days ...
-
bioRxiv - Bioengineering 2022Quote: ... were cultured in Endothelial Basal Medium-2 (EBM-2, Lonza, MD) and supplemented with an Endothelial Growth Media-2 (EGM-2 ...
-
bioRxiv - Cell Biology 2021Quote: ... were cultured in endothelial cell growth medium 2 (EGM-2, Lonza)) ...
-
bioRxiv - Microbiology 2022Quote: ... were supplemented with FGM-2 growth medium-2 BulletKit™ (Lonza). Plasmids constructs encoding codon-optimized cDNA for MCPyV LT ...
-
bioRxiv - Genetics 2023Quote: ... supplemented with Smooth muscle Medium-2 (SmGM-2, CC-4149, Lonza). Briefly ...
-
bioRxiv - Bioengineering 2023Quote: ... The modified EGM-2 medium contained EGM-2 basal medium (Lonza), 2% KOSR ...
-
bioRxiv - Cell Biology 2024Quote: ... Dorsomorphin (2 µM; StemMACS) and A-83-01 (2 µM; Lonza)) ...
-
An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... which consists of Endothelial Growth Basal Medium-2 (EBM-2; Lonza), SingleQuots supplements (all except hydrocortisone and gentamicin-1000 ...
-
bioRxiv - Cell Biology 2021Quote: ... PBMCs were seeded onto collagen I-coated wells at a cell density of 1.5 - 2 × 106 cells/cm2 in endothelial growth media 2 (EGM-2) medium (Lonza) supplemented with 16% defined fetal bovine serum (FBS ...