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Citations for Lonza :
1 - 47 of 47 citations for Recombinant Rat Sele His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Cell Biology 2022Quote: Rat Primary hepatocytes (Wister, Lonza, RICP01) were cultured as instructed ...
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bioRxiv - Cancer Biology 2020Quote: ... recombinant Cas9 nuclease (IDT) and the 4D-Nucleofector (Lonza) as previously described (Wagenblast et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant insulin (20 μg/mL, Lonza, #BE03-033E20), human recombinant EGF (2 ng/mL ...
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bioRxiv - Biochemistry 2020Quote: ... Rat vascular SMCs (Lonza, R-ASM-580) were grown in DMEM with 20% FBS and 1x Antibiotic-Antimyotic (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Co-cultures were done in IMDM supplemented with 5% HI-HS (Lonza) and gentamycin (86 μg/mL ...
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bioRxiv - Cell Biology 2022Quote: U2OS cells stably expressing GFP-tagged paxillin were cultured in phenol-red free DMEM (Lonza) at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... human and rat stellate cells were purchased from Lonza, and each were cultured according to their respective vendor protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Rat Aortic Smooth Muscle cells (RASMC) (Lonza, Walkersville, MD) and meticulously maintained in DMEM (Gibco from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid was transfected into HT29 cells using 4D-Nucleofecor (Lonza).
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bioRxiv - Biophysics 2020Quote: ... hydrocortisone and recombinant human epidermal growth factor (MEGM Bullet Kit CC-4136; Lonza). Cells were maintained in an incubator under standard conditions (36.5 °C ...
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bioRxiv - Neuroscience 2019Quote: ... cells were re-suspended in a rat neuron transfection buffer (Lonza) and mixed with 10 μg of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza) and Amaxa Nucleofector® 1 device in the presence or absence of flagellin (1μg ...
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bioRxiv - Bioengineering 2023Quote: The PyroGene Recombinant Factor C Endpoint Fluorescent Assay (Lonza, Walkersville, MD, cat # 50-658U) was performed as recommended ...
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 1 μg of Ssp1-linearized pcDNA4/TO Strep- tagged ADAP1 or GFP vector using Nucleofector2b (Lonza). Positive clones were selected with 10 μg/mL Blasticidin and 100 μg/mL Zeocin ...
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bioRxiv - Bioengineering 2023Quote: ... hepatocytes were electroporated using 100 µL Mouse/Rat Hepatocyte Nucleofector solution (Lonza) and conditions ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged TRIM21 (pFastBac-MBP-TRIM21) was expressed in SF9 insect cells in Lonza Insect-XPRESS according to standard protocols (Lonza). Cleared cell lysates were prepared by sonication of cell pellets in 50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...
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bioRxiv - Neuroscience 2022Quote: ... Nucleofected striatal neurons were transfected using manufacturer’s instructions (Rat Neuron Nucleofector Kit, Lonza) for rat hippocampal neurons before plating ...
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bioRxiv - Immunology 2022Quote: ... Absence of endotoxins (< 1EU/mL) was confirmed using the PyroGene™ Recombinant Factor C Assay (Lonza) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... cell pellet was resuspended in 100 ml Nucleofector™ (Rat Neuron Nucleofector kit, Lonza) transfection reagent and electroporated with 2 μg of cDNA mix according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... RNPs containing recombinant CRISPR/Cas9 and sgRNA were transfected to human cell lines using a Nucleofector (Lonza). Stable cell lines were generated by selection of hygrogmycin resistance ...
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bioRxiv - Molecular Biology 2020Quote: ... Days 16-24 media contained recombinant Oncostatin M (20ng/ml, Thermo) in HCM media lacking EGF (Lonza).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 100 μg/ml streptomycin and 15 ng/ml recombinant human macrophage colony stimulating factor (Lonza, Melbourne, Australia) on 10 mm square dishes (Bio-strategy ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA and recombinant CAS9 were delivered as ribonucleoprotein (RNP) complexes using a 4D-Nucleofector X-Unit (Lonza). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant plasmids were verified by Sanger sequencing and electroporated into E14 cells using the Nucleofector II platform (Lonza) (program A-013) ...
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bioRxiv - Cell Biology 2019Quote: ... Neurons were transfected before plating by using Amaxa Rat Neuron Nucleofection Kit (Lonza VPG-1003) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... were cultured on rat tail collagen I coated plates in EBM2 Endothelial Growth Medium (Lonza). Cells were treated with 10μg of EVs per 3×105 cells for 24 hours prior to angiogenesis and migration assays ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg ml−1 streptomycin and 15 ng ml−1 recombinant human macrophage colony stimulating factor (Lonza, Melbourne, Australia) on 10 mm square dishes (Bio-strategy ...
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bioRxiv - Bioengineering 2023Quote: ... gRNAs were complexed with recombinant Cas9 (IDT Cat#1081059) and introduced into cells by electroporation (4D-Nucleofector, Lonza Biosciences) according to manufacturer protocols ...
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bioRxiv - Biochemistry 2023Quote: ... Sf21 cells seeded at 500,000 cells/mL were transfected with recombinant bacmid DNA to generate the initial baculovirus (P1) in Insect-XPRESSTM Protein-free Insect Cell Medium (Lonza). The P1 virus was amplified two times (P2 and P3 ...
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bioRxiv - Neuroscience 2020Quote: ... The electroporation of freshly dissociated newborn hippocampal neurons was conducted using rat neuron nucleofection reagents (Amaxa, Lonza, Germany) with the 5×SRE-Luc reporter plasmid (Stratagene ...
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bioRxiv - Neuroscience 2021Quote: ... rat hippocampal neurons from E18 embryos were electroporated before plating with 1.5 μg of DNA using Nucleofector system (Lonza). For DNA-PAINT ...
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bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
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bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
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bioRxiv - Neuroscience 2021Quote: E18 rat hippocampal neurons were electroporated with GCaMP7f or Xph20-GCaMP7f and Homer1c-Dsred using the 4D Nucleofection system (Lonza) at DIV0 ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated fetal ECFC and MSC were cultured on rat tail collagen coated tissue-culture flasks in Endothelial Growth Medium (EGM2) (Lonza) with 10% of FBS ...
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bioRxiv - Cell Biology 2021Quote: ... or mutant) plasmids (4µg) were introduced to 4×106 dissociated neurons using Amaxa rat nucleofector kit (Lonza Bioscience, VPG-1003). Amaxa-nucleofected neurons plated at 500,000 cells per 35mm poly-D-lysine-coated glass bottom dish (WillCo Wells ...
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bioRxiv - Molecular Biology 2020Quote: ... Freshly isolated HBECs were plated onto rat tail collagen type I-coated (10 μg/ml) dishes in Bronchial Epithelial Growth Medium (BEGM, Lonza) containing 1% antibiotic– antimycotic solution (AA ...
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bioRxiv - Neuroscience 2022Quote: Neurons were nucleofected with identified plasmids prior to plating at 0 DIV using Amaxa Rat Neuron Nucleofector kit (DGP-1003; Lonza) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat cardiomyocyte Nucleofector Kit (Lonza, VAPE-1002) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2023Quote: ... Neurons were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat Neuron Nucleofector Kit (Lonza, VPG-1003) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
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bioRxiv - Cell Biology 2021Quote: Liver spheroids were prepared as previously described (Leite et al., 2016) except spheroids were formed from primary rat hepatocytes (Lonza, cat# RSCP01) and primary human HSCs ...
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bioRxiv - Neuroscience 2020Quote: ... dissociated ganglia were pelleted at 100 x g for 5 min and resuspended in 100 µl ‘Nucleofector solution’ (Rat Neuron Nucleofector kit; Lonza, Alpharetta, GA). 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG ...
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bioRxiv - Cancer Biology 2019Quote: All tumouroids were fabricated using monomeric Type I rat-tail collagen (First Link, Birmingham, UK) and the RAFT™ protocol pages 8-9 (Lonza, Slough, UK) as previously described21 ...