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Citations for Lonza :
1 - 50 of 4943 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... macrophage” programmed in 4D-NucleofectorTM (Lonza). Cells were cultured in the media composed of 50% L929-cell conditioned media and 50% cRPMI ...
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bioRxiv - Cell Biology 2023Quote: ... we assessed cell death (ToxiLight assay, Lonza, LT07-117) and cell viability (MUH cell viability assay [2]).
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bioRxiv - Cancer Biology 2021Quote: ... FF-120 or CM-130 respectively – the preset programmed in 4D-nucleofector system (Lonza). The preparation of the cell suspension and the process of the electroporation are similar to that described in the CRISPR method section ...
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bioRxiv - Genomics 2024Quote: ... mRNA:gRNA were added directly to 1×106 cells resuspended in 20 µl of P3 solution and nucleofected with pre-programmed pulse EO-115 in 4D-NucleofectorTM System (Lonza). After nucleofection ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatants were collected 3 and 6 days post-infection and induced-cell death and viral titers were determined in the ToxiLight Bioassay (Lonza) and PFA ...
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bioRxiv - Microbiology 2021Quote: ... cells were overlaid with 1:1 mixture of 2% agarose (Lonza, Basel, Switzerland) and 2X MEM medium ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were harvested with Accutase and 2-3×106 cells were resuspended in 100µl mouse neural stem cell nucleofector buffer (Lonza) with 2µg plasmid DNA ...
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bioRxiv - Immunology 2021Quote: ... HUVEC cells were cultured on a 1% gelatin coated surface in EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM (Lonza) following the manufacturer’s instructions with an additional 6% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... JAWS transfection was achieved using 20 μg DNA and 2 x 106 million cells per 100 μl nucleofection solution from the Amaxa Nucleofector Mouse Dendritic Cell kit (Lonza), and the Y-001 program ...
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bioRxiv - Cell Biology 2022Quote: U2OS cells stably expressing GFP-tagged paxillin were cultured in phenol-red free DMEM (Lonza) at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The recombinant plasmid was transfected into HT29 cells using 4D-Nucleofecor (Lonza).
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bioRxiv - Biochemistry 2024Quote: ... Cells were split at a 1:2 ratio every 2 days by trypsinization (Lonza cat# CC-5012), followed by centrifugation at 200 x g for 3 min before resuspending and reseeding in EBM Basal Medium supplemented with the EGM Singlequots Supplement Pack ...
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bioRxiv - Bioengineering 2020Quote: ... The cell suspension contained a 2:1 ratio of GFP-HUVECs (Lonza) and HDFs ...
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bioRxiv - Cell Biology 2022Quote: ... BUB-1 and CLS-2::GFP production was performed in 2 L SF9 cells in Insect-XPRESS (Lonza) medium (1×10^6 cells/mL) ...
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bioRxiv - Synthetic Biology 2021Quote: ... and γδ T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 medium (Lonza) (5% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×10E6 ME-1 cells were nucleofected with CRISPR/Cas9 plasmids (2 μg each) using Nucleofector Technology (Lonza Biologics) with the program X-01 and Amaxa Cell Line Nucleofector Kit V ...
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bioRxiv - Bioengineering 2022Quote: ... Endothelial Cell Growth Medium-2 (EGM-2, Lonza, Switzerland) was used as the medium ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Cancer Biology 2021Quote: ... Stable LBH-overexpressing BT549 cell lines were generated by nucleofection of 2×106 BT549 cells with 2 μg linearized pCDNA3 or pCDNA3+Lbh 13 and 1 μg pEGFP (Lonza) in Solution V (Lonza) ...
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bioRxiv - Systems Biology 2021Quote: ... TIME cells were cultured in Endothelial Cell Growth Medium-2 BulletKitTM (EGM-2, Lonza). All cell cultures utilized in this study were maintained at 5% CO2 atmosphere and 37°C.
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bioRxiv - Cell Biology 2021Quote: ... PBMCs were seeded onto collagen I-coated wells at a cell density of 1.5 - 2 × 106 cells/cm2 in endothelial growth media 2 (EGM-2) medium (Lonza) supplemented with 16% defined fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... These cells are mantained in 30% FBS/1% antibiotic/antimycotic /1% Non-essential AA/ EGM-2 media (Lonza) with Laminin521 coating (5 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in EBM-2/EGM-2 medium (Lonza) with 12% FBS ...
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bioRxiv - Genomics 2023Quote: ... were purchased from ATCC and cultured in endothelial cell growth basal medium-2 containing bullet kit growth factor supplements (EBM-2 [endothelial cell growth basal medium-2]; Lonza), 5% fetal bovine serum ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were grown in Endothelial Cell Growth Medium BulletKit®-2 (EGM-2 BulletKit, Lonza) at 37°C in an atmosphere of 5% CO2 and 95% air ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM Ultraglutamine 1 (Lonza) and 1× MycoZap antibiotics (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... 100 μg ml−1 streptomycin and 15 ng ml−1 recombinant human macrophage colony stimulating factor (Lonza, Melbourne, Australia) on 10 mm square dishes (Bio-strategy ...
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bioRxiv - Microbiology 2021Quote: ... HPMEC were cultured in endothelial cell growth basal medium 2 supplemented with an Endothelial Cell Growth Medium-2 (EGM-2™) supplemental bullet kit (Lonza). Cells were maintained at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... were electroporated with 1 μg of Ssp1-linearized pcDNA4/TO Strep- tagged ADAP1 or GFP vector using Nucleofector2b (Lonza). Positive clones were selected with 10 μg/mL Blasticidin and 100 μg/mL Zeocin ...
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bioRxiv - Immunology 2020Quote: ... Rinsed cells were resuspended in buffer P4 (mouse) or P2 (human) at 1-10 x 106 cells/20 μL (Lonza). Non-targeting Alt-R crRNA #1 ...
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bioRxiv - Immunology 2021Quote: ... Naïve T cells expressing Cas9 were washed once with 1 mL pre-warmed recovery medium (Mouse T Cell Nucleofector Medium, Lonza) before electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were trypsinized and resuspended in endothelial cell growth basal medium-2 (EBM-2, Lonza, Switzerland). Cell seeding density into wells was 0.6 × 105 cells in 300μl medium ...
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bioRxiv - Cell Biology 2020Quote: ... EBM-2 (Endothelial Cell Growth Basal Medium-2, Lonza, #CC-3156) was supplemented with EGM-2 SingleQuots Supplements (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in endothelial cell growth medium 2 (EGM-2, Lonza)) ...
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bioRxiv - Physiology 2024Quote: ... Endothelial cells were grown in endothelial cell basal medium (EBM-2) supplemented with an endothelial cell bullet kit (EGM-2) (Lonza) and used between passage 3 and 5 in tissue culture dishes coated with 0.1% gelatin and maintained at 37°C in a humidified incubator at 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged TRIM21 (pFastBac-MBP-TRIM21) was expressed in SF9 insect cells in Lonza Insect-XPRESS according to standard protocols (Lonza). Cleared cell lysates were prepared by sonication of cell pellets in 50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... Sf21 cells (2 × 106 cells mL−1) were infected with the P3 viral stock in Insect-XPRESS protein-free medium (Lonza). The infected insect cells were grown at 27 °C with shaking for 66 hours ...
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bioRxiv - Cell Biology 2020Quote: ... were resuspended at a density of 6×106 cells mL−1 in Microvascular Endothelial Cell Growth Medium-2 media (EGM-2MV, Lonza). The bottom channel of the chip was washed with EGM-2MV and loaded with 6 μL of HIMEC cell suspension (~36,000 cells per chip) ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary HBECs (human bronchial epithelial cells) were grown in 2% oxygen using a 1:1 mixture of bronchial epithelial basal medium (BEBM; Lonza) and DMEM and supplemented with SingleQuot (Lonza ...
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bioRxiv - Cancer Biology 2019Quote: ... Human umbilical vein endothelial cells (HUVEC) were grown in endothelial cells growth medium-2 (EGM-2) (Lonza). All cells were cultured at 37◻°C and 5% CO2 in incubator cells.
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bioRxiv - Molecular Biology 2024Quote: ... Cells were cultured in EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, CC-3162) and plated in T75 culture flask at 5000 cells/ cm2 with iMatrix-511 (Nacalai USA #892021).
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bioRxiv - Bioengineering 2021Quote: ... the cells were expanded in EGM-2 medium with 2% FBS (Lonza) and plated at 10,000 cells/well in a 96 well plate ...
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bioRxiv - Pathology 2020Quote: ... were cultured in Endothelial Cell Growth Medium-2 media (EGM-2) (Lonza). HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were cultured in endothelial cell growth medium-2 (EGM-2, Lonza) with 100 U mL−1 penicillin and 100 U mL−1 streptomycin (Nacalai Tesque) ...
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bioRxiv - Biophysics 2023Quote: ... were cultured in Endothelial Cell Growth Medium-2 BulletKit (EGM-2; Lonza) and used between passages 4 to 7.
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in Endothelial Cell Growth Medium-2 BulletKit (EGM-2; Lonza) at 37°C in a humidified atmosphere of 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... were cultured in Endothelial Cell Growth Medium-2 BulletKit (EGM-2; Lonza) at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were obtained from Lonza and maintained in endothelial cell basal medium-2 plus 1% FBS and essential growth factors (Lonza).
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bioRxiv - Genetics 2021Quote: ... RNPs containing recombinant CRISPR/Cas9 and sgRNA were transfected to human cell lines using a Nucleofector (Lonza). Stable cell lines were generated by selection of hygrogmycin resistance ...
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bioRxiv - Developmental Biology 2021Quote: ... 2×106 iPSC single cells were transfected using the Amaxa Human Stem Cell Nucleofector® Kit 2 (Lonza) with 4 μg ...