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Citations for Lonza :
1 - 50 of 310 citations for Rat TRPV1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 5 µg of NOSIP shRNA plasmids or control TRC2 plasmid was mixed with 100 µl Cell Line Nuceleofector Solution V (Lonza) and added to one million cells ...
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bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
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bioRxiv - Cell Biology 2021Quote: ... or mutant) plasmids (4µg) were introduced to 4×106 dissociated neurons using Amaxa rat nucleofector kit (Lonza Bioscience, VPG-1003). Amaxa-nucleofected neurons plated at 500,000 cells per 35mm poly-D-lysine-coated glass bottom dish (WillCo Wells ...
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bioRxiv - Neuroscience 2022Quote: Neurons were nucleofected with identified plasmids prior to plating at 0 DIV using Amaxa Rat Neuron Nucleofector kit (DGP-1003; Lonza) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat cardiomyocyte Nucleofector Kit (Lonza, VAPE-1002) according to the manufacturer’s instruction.
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bioRxiv - Neuroscience 2023Quote: ... Neurons were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat Neuron Nucleofector Kit (Lonza, VPG-1003) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Rat Primary hepatocytes (Wister, Lonza, RICP01) were cultured as instructed ...
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bioRxiv - Biochemistry 2020Quote: ... Rat vascular SMCs (Lonza, R-ASM-580) were grown in DMEM with 20% FBS and 1x Antibiotic-Antimyotic (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... human and rat stellate cells were purchased from Lonza, and each were cultured according to their respective vendor protocols ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Rat Aortic Smooth Muscle cells (RASMC) (Lonza, Walkersville, MD) and meticulously maintained in DMEM (Gibco from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with pcDNA3 HA eIF4GI plasmid or control green fluorescent protein (GFP)-expressing plasmid (pMAX-GFP plasmid, Lonza). pcDNA3 HA eIF4GI (1–1599 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 ug PX459 plasmid and blasticidin-expressing plasmid in Amaxa buffer (Lonza) with 100 mM ATP ...
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bioRxiv - Genomics 2020Quote: ... sgRNA plasmid and donor plasmid on Amaxa 4D-Nucleofactor program CA-137 (Lonza). Following nucleofection ...
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bioRxiv - Neuroscience 2020Quote: ... hESCs human cortical neurons were nucleofected with shRNAs constructs against ASH1L using the X-unit of the Amaxa nucleofector unit (Lonza, catalog # AAF1002X). Human neurons between days 30 to 32 of neuronal induction were single cell dissociated using single cell dissociation reagent (stem cell technologies CT catalog # 07174 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 μg of each pX330-gRNA plasmid plus 0.1 μg of pmaxGFP plasmid (Lonza) were transiently transfected into exponentially growing cells using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... using pMAX-GFP plasmid (Lonza) as template ...
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bioRxiv - Neuroscience 2019Quote: ... cells were re-suspended in a rat neuron transfection buffer (Lonza) and mixed with 10 μg of cDNA ...
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bioRxiv - Cell Biology 2024Quote: ... Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza) and Amaxa Nucleofector® 1 device in the presence or absence of flagellin (1μg ...
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bioRxiv - Bioengineering 2023Quote: ... hepatocytes were electroporated using 100 µL Mouse/Rat Hepatocyte Nucleofector solution (Lonza) and conditions ...
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bioRxiv - Genomics 2023Quote: ... 0.25 µg GFP plasmid (pmaxGFP, Lonza), and 8.25 µL TransIT-X2 (Mirus ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 μg of pmaxGFP plasmid (Lonza) was added to transfection mixes to monitor transfection efficiency ...
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bioRxiv - Neuroscience 2022Quote: ... Nucleofected striatal neurons were transfected using manufacturer’s instructions (Rat Neuron Nucleofector Kit, Lonza) for rat hippocampal neurons before plating ...
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bioRxiv - Molecular Biology 2023Quote: ... program A-30 with 10 µg of plasmid DNA and 1 µg of eGFP control plasmid (Amaxa, Lonza) was used ...
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bioRxiv - Microbiology 2021Quote: ... The pmaxGFP plasmid was obtained from Lonza.
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bioRxiv - Genomics 2021Quote: ... and pMaxGFP plasmid at 200ng/ul (Lonza) using solution P3 and program CA-137.
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bioRxiv - Cancer Biology 2019Quote: ... 5 × 106 cells were co-transfected with 5 μg of pSpCas9(BB)– 2A–Puro:sgRNA #4 plasmid and 5 μg of pCSII–CMV:A3B–3×FLAG–IRES–EGFP donor DNA plasmid using the Amaxa Nucleofector (Lonza) with nucleofection solution R ...
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bioRxiv - Neuroscience 2019Quote: ... and 2.5 μg of CRISPR:hSpCas9 plasmid and 2.5 μg linearized DNA targeting plasmid and immediately pulse with program DC100 (using a 4D-Nucleofector, Lonza). Cells were resuspended in warm mTeSR1 (Stem cell technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... H9 cells were transfected with the PB-GFP-P2A-ΔNβCAT plasmid and a Piggybac transposase-coding plasmid using the P3 primary cell 4D nucleofector kit (Lonza). After 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellet was resuspended in 100 ml Nucleofector™ (Rat Neuron Nucleofector kit, Lonza) transfection reagent and electroporated with 2 μg of cDNA mix according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were dissociated into single cells with accutase and 1 million cells were used for nucleofection with 5 μg of gRNA/Cas9 plasmid and 5 μg of each donor plasmid (WT and G12D) using Nucleofector II (Lonza) and program B-16 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.5-1 μL of a solution containing 1 μg/μL of pCAG-PBase plasmid and 1.5 μg/μl of pPbCag-GCaMP6s plasmid in 1X phosphate buffered saline (PBS; Lonza, Basel) and 0.0025% of the dye Fast Green (Sigma-Aldrich Co. ...
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bioRxiv - Bioengineering 2023Quote: Approximately 0.25 × 106 U2OS.eGFP-PEST cells were nucleofected with 300 ng of Cas9 expression plasmid and 30 ng of eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Bioengineering 2023Quote: ... and 50 ng of AcrIIA4 expression or 300 ng of Cas9-P2A-CSD-AcrIIIA4 plasmid and 30 ng eGFP-targeting gRNA expression plasmid using the SE Cell line 4D-Nucleofector X Kit (Lonza) and DN100 pulse program ...
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bioRxiv - Cancer Biology 2023Quote: ... Two gRNA plasmids and pCas9 (D10)-GFP plasmid were nucleofected into cells using Basic Nucleofector Kit for Primary Mammalian Epithelial Cells (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A9 ESCs harboring the docking platform at the Rosa26 locus were transfected by nucleofection with integrase φC31 plasmid and the respective exchange plasmid using an Amaxa 4D Nucleofector (Lonza). The integration leads to replacement of the hygromycin resistance gene by the βGEO-STOP cassette and the HDAC1-FLAG ORF ...
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bioRxiv - Developmental Biology 2021Quote: ... PiggyBac plasmids were introduced using Amaxa Nucleofector (Lonza). After antibiotics selection ...
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bioRxiv - Developmental Biology 2019Quote: ... These plasmids were nucleofected (Amaxa 4D-Nucleofector, Lonza) into A2Lox cells (gift from Prof ...
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bioRxiv - Biochemistry 2020Quote: ... Complete digestion of the pMax-GFP plasmid (Lonza) was confirmed by agarose gel electrophoresis prior to transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Co-transfection of cells with pMAXGFP plasmid (Lonza) was conducted in a 1:1 ratio to provide a normalisation control for transfection efficiency.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... and 200 ng pMaxGFP expression plasmid (Lonza, #13429329) via nucleofection (Lonza ...
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bioRxiv - Genomics 2020Quote: ... and 10 ng of GFP-max plasmid (Lonza) diluted to 12.5 μL total volume in Opti-MEM reduced serum media (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Neurons were transfected before plating by using Amaxa Rat Neuron Nucleofection Kit (Lonza VPG-1003) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... were cultured on rat tail collagen I coated plates in EBM2 Endothelial Growth Medium (Lonza). Cells were treated with 10μg of EVs per 3×105 cells for 24 hours prior to angiogenesis and migration assays ...
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bioRxiv - Cancer Biology 2021Quote: ... These plasmids were co-transfected with GFP (pMAXGFP, Lonza), and single cells were sorted by FACS into 96-well plates ...
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bioRxiv - Physiology 2023Quote: ... 0.25 µg reporter plasmid pMax-GFP (Lonza, Basel, Switzerland) and 1.25 µg plasmid of the VGSC to be examined (N/P ratio ≈ 5) ...
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Antigen Geometry Tunes Mast Cell Signaling Through Distinct FcεRI Aggregation and Structural ChangesbioRxiv - Biophysics 2023Quote: ... Plasmid transfections were performed using the Amaxa system (Lonza) with Solution L (Lonza VCA-1005 or MirusBio Ingenio solution MIR-50114 ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were transfected with plasmid DNA using Nucleofector (Lonza) before plating as described (Minegishi et al. ...
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bioRxiv - Immunology 2024Quote: ... the turbogfp gene was amplified from pmaxGFP plasmid (Lonza) using primer 5 and 6 ...
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bioRxiv - Cancer Biology 2019Quote: ... NSCs were transfected with plasmids by nucleofection (AMAXA 2B, Lonza) using programme A-033 ...