Labshake search
Citations for Lonza :
1 - 50 of 1644 citations for Rat IL 18 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza) and Amaxa Nucleofector® 1 device in the presence or absence of flagellin (1μg ...
-
bioRxiv - Neuroscience 2022Quote: ... Nucleofected striatal neurons were transfected using manufacturer’s instructions (Rat Neuron Nucleofector Kit, Lonza) for rat hippocampal neurons before plating ...
-
bioRxiv - Bioengineering 2023Quote: ... with two phosphothiorate linkages on each end were mixed with 82 µL Nucleofector Solution V and 18 µL of Supplement Solution 1 according to manufacturer’s recommendations for the Cell Line Nucleofector Kit V (Lonza). 5×105 U2OS.EGFP parent cells were resuspended in the above mixture and nucleofected with a Lonza Nucleofector 2b Device (Kit V ...
-
bioRxiv - Neuroscience 2022Quote: ... cell pellet was resuspended in 100 ml Nucleofector™ (Rat Neuron Nucleofector kit, Lonza) transfection reagent and electroporated with 2 μg of cDNA mix according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... T cells were resuspended in P3 buffer (0.75-1 x 106 cells per 18 μl P3) from the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). 10 μg/μl Alt-R S.p ...
-
bioRxiv - Cell Biology 2019Quote: ... Neurons were transfected before plating by using Amaxa Rat Neuron Nucleofection Kit (Lonza VPG-1003) following manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... supplemented with 0.1 μg/ml of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
-
bioRxiv - Cell Biology 2023Quote: ... supplemented with 0.1 μg/mL of interleukin (IL)-34 (IL-34) (Lonza, Basel-Stadt, Switzerland), 0.01 μg/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
-
bioRxiv - Cell Biology 2020Quote: ... following DOX treatment (18 h) cells were starved in DMEM (Lonza) lacking methionine (Met ...
-
bioRxiv - Cell Biology 2022Quote: Rat Primary hepatocytes (Wister, Lonza, RICP01) were cultured as instructed ...
-
bioRxiv - Cell Biology 2021Quote: ... MEFs were immortalized with pBabe-SV40Tag by electroporation using the Amaxa Mouse/Rat Hepatocyte Nucleofector Kit (Lonza, VPL-1004). Immortalized MEF lines were genotyped ...
-
bioRxiv - Biochemistry 2020Quote: ... Rat vascular SMCs (Lonza, R-ASM-580) were grown in DMEM with 20% FBS and 1x Antibiotic-Antimyotic (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... or mutant) plasmids (4µg) were introduced to 4×106 dissociated neurons using Amaxa rat nucleofector kit (Lonza Bioscience, VPG-1003). Amaxa-nucleofected neurons plated at 500,000 cells per 35mm poly-D-lysine-coated glass bottom dish (WillCo Wells ...
-
bioRxiv - Neuroscience 2022Quote: Neurons were nucleofected with identified plasmids prior to plating at 0 DIV using Amaxa Rat Neuron Nucleofector kit (DGP-1003; Lonza) according to manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat cardiomyocyte Nucleofector Kit (Lonza, VAPE-1002) according to the manufacturer’s instruction.
-
bioRxiv - Neuroscience 2023Quote: ... Neurons were nucleofected with plasmids of interest using the Lonza 2b Nucleofector and Rat Neuron Nucleofector Kit (Lonza, VPG-1003) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2020Quote: ... dissociated ganglia were pelleted at 100 x g for 5 min and resuspended in 100 µl ‘Nucleofector solution’ (Rat Neuron Nucleofector kit; Lonza, Alpharetta, GA). 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG ...
-
bioRxiv - Bioengineering 2024Quote: The hMSCs were isolated from human bone marrow aspirate (Lonza; donor = 18-year-old black female) and serial expanded following previously described protocols[44] ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... human and rat stellate cells were purchased from Lonza, and each were cultured according to their respective vendor protocols ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Rat Aortic Smooth Muscle cells (RASMC) (Lonza, Walkersville, MD) and meticulously maintained in DMEM (Gibco from Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... 18-20μL of cells (4.5-20 *104 cells) were then plated into 16-well nucleocuvette strips (Lonza). Each well had also previously received 4μL of ribonucleoprotein ...
-
bioRxiv - Biochemistry 2021Quote: Plateable, cryopreserved primary human hepatocytes (pHep) from one male donor (18 years, BMI 28.7) were purchased from Lonza, Switzerland ...
-
bioRxiv - Neuroscience 2019Quote: ... cells were re-suspended in a rat neuron transfection buffer (Lonza) and mixed with 10 μg of cDNA ...
-
bioRxiv - Immunology 2021Quote: ... density gradient centrifugation (20 min, 800g) and culture for 12-18 h in X-VIVO 10 TM medium (Lonza) supplemented with gentamicin ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.5 μg ssODN and 1.2 μl 100 μM Alt-R Cas9 Electroporation Enhancer (IDT) were mixed and added to 20,000 cells resuspended in 18 μl SF Nucleofection Buffer (Lonza). Nucleofection mixture were transferred to 16-well Nucleocuvette strips (Lonza ...
-
bioRxiv - Bioengineering 2023Quote: ... hepatocytes were electroporated using 100 µL Mouse/Rat Hepatocyte Nucleofector solution (Lonza) and conditions ...
-
bioRxiv - Microbiology 2021Quote: ... between 1 x 106 and 1 x 107 cells were resuspended in 18 μl of P3 primary cells solution (Lonza), 2 μl of the restricted cassette (2-4 μg total ...
-
bioRxiv - Neuroscience 2020Quote: Primary human cortical astrocytes purified from 17-18 weeks gestation human fetuses of indeterminate sexes were used in this experiment (Lonza). Astrocytes were expanded using the astrocyte growth medium bulletkit according to the manufacturer’s instructions at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... Vero E6 cells with an average population of 104 cells were cultured for 18–24 h in each well in the growth medium [Eagle’s Minimum Essential Medium (EMEM) (Lonza Bioscience) +L serum (FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... An iCasp9-CD20/CD19-CAR transposon minicircle carrying CD20 scFv[17] and CD19 scFv[18] and a qPBase plasmid were delivered into activated T cells using 4D-Nucleofector device (Lonza) and Quantum Nufect Kit (GenomeFrontier Therapeutics ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were pelleted and resuspended in 100 μl of P3 transfection solution (82 μl Amaxa Buffer and 18 μl P3 supplement; Lonza) and 10 μl of DNA mix ...
-
bioRxiv - Immunology 2022Quote: ... 1 million NK cells were resuspended in 20 ul 4D nucleofector master mix (82% P3 + 18% supplement 1; Lonza, V4XP-3032) and then mixed with Cas12a RNPs for electroporation using program CM137 (87) ...
-
bioRxiv - Systems Biology 2024Quote: Human bone marrow MSCs (from seven donors, 18-25 years of age) were isolated from human bone marrow mononuclear cells (Lonza Biosciences) and cultured and phenotype-tested as described previously 10 ...
-
bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 million NK cells were resuspended in 20 μl Amaxa 4D nucleofector master mix (82% P3 + 18% supplement 1) (Lonza, V4XP-3032), then mixed with Cas12a RNPs for electroporation using program CM137 ...
-
bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
-
bioRxiv - Biochemistry 2024Quote: 1 µL of 63 µM Alt-R™ S.p.Cas9 V3 (IDT: 10007807) was diluted with 18 µL of SE Cell Line Nucleofector® Solution (Lonza V4XC-1032), followed by the addition of 1.8 µL of 100 µM sgRNA (3:1 sgRNA:Cas9) ...
-
bioRxiv - Neuroscience 2020Quote: ... culture media supplemented with 0.1μg/ml of inter-leukin (IL)-34 (Lonza, Basel-Stadt, Switzerland) and 0.01μg/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF ...
-
bioRxiv - Bioengineering 2022Quote: ... were cultured on rat tail collagen I coated plates in EBM2 Endothelial Growth Medium (Lonza). Cells were treated with 10μg of EVs per 3×105 cells for 24 hours prior to angiogenesis and migration assays ...
-
bioRxiv - Neuroscience 2020Quote: ... The electroporation of freshly dissociated newborn hippocampal neurons was conducted using rat neuron nucleofection reagents (Amaxa, Lonza, Germany) with the 5×SRE-Luc reporter plasmid (Stratagene ...
-
bioRxiv - Immunology 2023Quote: ... at bead-to-cell ratio of 1:1 in human IL-2 medium (X-VIVO 15, serum-free hematopoietic cell medium, with 2mM L-Glutamine and gentamicin (Lonza) supplemented with 5% Human AB serum (Valley Medical) ...
-
bioRxiv - Neuroscience 2021Quote: ... rat hippocampal neurons from E18 embryos were electroporated before plating with 1.5 μg of DNA using Nucleofector system (Lonza). For DNA-PAINT ...
-
bioRxiv - Neuroscience 2020Quote: ... 4-6 μg of plasmid was electroporated using the AMAXA Nucleofector device (Neurons Rat DRG, G-013 program; Lonza) before plating and maintained for 48 h.
-
bioRxiv - Neuroscience 2021Quote: E18 rat hippocampal neurons were electroporated with GCaMP7f or Xph20-GCaMP7f and Homer1c-Dsred using the 4D Nucleofection system (Lonza) at DIV0 ...
-
bioRxiv - Neuroscience 2021Quote: ... The isolated fetal ECFC and MSC were cultured on rat tail collagen coated tissue-culture flasks in Endothelial Growth Medium (EGM2) (Lonza) with 10% of FBS ...
-
bioRxiv - Molecular Biology 2020Quote: ... Freshly isolated HBECs were plated onto rat tail collagen type I-coated (10 μg/ml) dishes in Bronchial Epithelial Growth Medium (BEGM, Lonza) containing 1% antibiotic– antimycotic solution (AA ...
-
bioRxiv - Neuroscience 2023Quote: ... TG trigeminal ganglion neurons were transfected with 3 µg of NaV1.7-PEPCy3 plasmid and 0.75 µg green fluorescent protein (GFP) using the rat neuron 4D-Nucleofector solution (P3 Primary Cell Solution, program DR 114; Lonza Biosciences). Neurons were plated on round bottom glass dishes (cat ...
-
bioRxiv - Cell Biology 2021Quote: Liver spheroids were prepared as previously described (Leite et al., 2016) except spheroids were formed from primary rat hepatocytes (Lonza, cat# RSCP01) and primary human HSCs ...
-
bioRxiv - Cancer Biology 2019Quote: All tumouroids were fabricated using monomeric Type I rat-tail collagen (First Link, Birmingham, UK) and the RAFT™ protocol pages 8-9 (Lonza, Slough, UK) as previously described21 ...