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Citations for Lonza :
1 - 50 of 1695 citations for Protein Concentration kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Neuroscience 2019Quote: ... 8 × 105 cells were co-transfected with 3 µg plasmid-sgRNA construct and ssODN (0.6 µM final concentration) with Amaxa Human Stem Cell Nucleofector Kit 1 (Lonza) and program A-023 for the Amaxa Nucleofector Device II ...
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bioRxiv - Microbiology 2022Quote: ... and resuspended in 100 μL nucleofector solution at a concentration of 1.5 x 106 cells/100 μl following the instructions of the Nucleofector Kit V (Lonza). In vitro transcribed RNA (5 μg ...
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bioRxiv - Immunology 2023Quote: Hepatocytes (7×105 cells) were transfected with various plasmids (at 6 μM or indicated concentrations) using the Mouse/ Rat hepatocyte Nucleofector kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and heparin in proprietary concentrations) (Lonza #CC-3162 ...
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bioRxiv - Immunology 2022Quote: LPS concentration in serum was determined using a chromogenic assay based on a Limulus amebocyte extract (LAL kit-terminal QCL1000) (LONZA, Basel, Switzerland). Samples were collected at the time of euthanasia via cardiac puncture to try to minimize as much as possible the chance of contamination ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of low melting agarose (SeaKem LE, Lonza) for plug preparation was reduced to 0.8% and lysozyme (1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... and 2000 p mol of a degron-tagged control protein (mTagBFP2-RxxG) in a 100 µl nucleofection reaction (4D nucleofector kit SE plus supplement SF1, Lonza) 14 hours following nucleofection ...
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bioRxiv - Cell Biology 2019Quote: To induce expression of fluorescently labeled proteins DCs were transfected according to manufacturer guidelines using nucleofector kit for primary T cells (Amaxa, Lonza Group). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Single iPSCs were then nucleofected with the pX459v2 plasmid coding for the Cas9 protein and the sgRNA using P3 primary Cell 4D nucleofector kit (Lonza, V4XP-302). After nucleofection cells were plated on a 6-well plate in E8 flex medium supplemented with RevitaCell ...
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bioRxiv - Cancer Biology 2022Quote: ... with final concentrations as indicated: X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Genomics 2020Quote: ... at a final concentration of 1.5% in PBS (Lonza, BE17-516F) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... for a final concentration of 3.5% SeaPlaque agarose (Lonza, Basel, Switzerland) in LDNT media [72] ...
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bioRxiv - Cancer Biology 2024Quote: ... with final concentrations as indicated : X-VIVO 10 (Lonza, BE04-380Q) supplemented with 20% BIT 9500 Serum Substitute (StemCell technologies ...
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bioRxiv - Immunology 2023Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...
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bioRxiv - Molecular Biology 2021Quote: ... we used a loading concentration of 450 cells/µl in 1xPBS (Lonza) with 15% Optiprep (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... The EVs concentration process was described in the manufacture’s protocol (HansaBioMed/Lonza) and EVs were finally diafiltrated in RNase-free PBS.
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Neuroscience 2022Quote: ... at a concentration of 10 × 106 cells/petri dish in RPMI1640 medium (Lonza) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: ... with a final concentration of 2% formaldehyde and 2% NuSieve 3:1 agarose (Lonza). 5 µg RNA was denatured for 10 min at 70 °C with 1 X MOPS buffer (20 mM MOPS ...
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bioRxiv - Genomics 2021Quote: ... 1.6×105 cells were suspended in P3 Primary Cell Solution with 50pmol Cas9 protein (St. Jude Protein Production Core) and 150pmol sgRNA (Synthego) with pMaxGFP plasmid at 200ng/ul (Lonza) in a total volume of 20ul with program DS130 ...
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bioRxiv - Genomics 2021Quote: ... and 50 pmole of spCas9 protein (St. Jude Protein Production Core) with pMaxGFP plasmid as a transfection control at 200ng/ul (Lonza) using the 4D-Nucleofector™ X-unit (Lonza ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin-streptomycin (penicillin 50 U/ml and streptomycin 50 μg/ml, final concentration; Lonza) at 37 °C with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: Fibroblast culture medium as described above was prepared at 2X concentration using 2X EMEM (Lonza). To prepare the bottom layer of agar plates ...
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bioRxiv - Immunology 2023Quote: ... suspended in a concentration of 1.5×106 in 100 µl cell line nucleofector solution T (Lonza) and nucleofected with 5 μg of a plasmid using program X-001 in the Nucleofector IIb device (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... ii) Adherent cell monolayers at an 80% confluence were dissociated with different concentrations trypsin-EDTA (LONZA), 10X (0,25% v/v ...
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bioRxiv - Bioengineering 2024Quote: ... cells were treated with various concentrations of BMP-2 or FK506 in an osteogenic media (Lonza). Cell culture medium was changed every 3 days ...
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bioRxiv - Bioengineering 2024Quote: ... they were treated with various concentrations of BMP-2 or FK506 in an osteogenic media (Lonza). The medium was replaced every 3-4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... and T cells were added at different concentrations for 48 h in X-VIVOTM 15 media (Lonza) supplemented with 5 % human AB serum (Gemini Bio ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were separated by SDS-PAGE using 14% Prosieve (Lonza) polyacrylamide gels and electroblotted to Immobilon-P membranes (Millipore) ...
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bioRxiv - Synthetic Biology 2023Quote: ... with Cas9 ribonucleic protein (RNP) complexes in SF buffer (Lonza), using the pulse codes CM-150 or CV-104 ...
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bioRxiv - Cancer Biology 2020Quote: ... consisting of 100 pmol of chemically modified sgRNA (Synthego) and 35 pmol of Cas9 protein (St. Jude Protein Production Core) via nucleofection (Lonza, 4D-Nucleofector™ X-unit) using solution P3 and program CA-137 in small cuvettes according to the manufacturers recommended protocol ...
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bioRxiv - Immunology 2021Quote: ... and suspended at a concentration of 3 × 106cells/mL in RPMI-1640 (BioWhittaker®, Lonza, Walkersville, MD, USA) containing 15% heat-inactivated horse serum ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were electroporated with 0.4 μg pmaxGFP plasmid and different concentrations of SGM and SAM (0.01, 0.1, 1 mM) using 4D-Nucleofector X Unit from Lonza. EH-100 program was used for HepG2 by following the manufactures protocol ...
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bioRxiv - Immunology 2023Quote: ... 24-well plate) at a concentration of 75,000 cells per well in Smooth muscle growth medium (SmGM) (Lonza) and left to adhere and spread for 90 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and replaced by Ultradoma Protein-Free (LONZA). The cultured medium was harvested and replenished on the second ...
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bioRxiv - Genetics 2022Quote: ... membranes were stained with SYPRO-Ruby Protein Gel Stain (Lonza Rockland) to confirm equal loading ...
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bioRxiv - Immunology 2023Quote: The 3 kbp DNA plasmid encoding green fluorescent protein (GFP) (Lonza) was used to assess transfection efficiency ...
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bioRxiv - Microbiology 2021Quote: ... 2x overlay media was added to the inoculum to give a final concentration of 2% (v/v) FBS / MEM media and 0.4% (w/v) SeaPrep Agarose (Lonza) to achieve a semi-solid overlay ...
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bioRxiv - Physiology 2023Quote: Purified HA was mixed with sucrose solution (final concentration of 333 mM) and loaded to a 0.4% SeaKem Gold agarose gel (Lonza). HA-Ladders (Hyalose ...
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bioRxiv - Cell Biology 2023Quote: ... S2Xpress cells were cultured in Insect-XPRESSTM protein-free medium (LONZA, Belgium) supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin (GIBCO) ...
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bioRxiv - Immunology 2023Quote: ... Protein expression was induced with 5µM CdCl2 in Insect-XPRESS medium (Lonza) for 5-7 days and supernatants were concentrated and purified using strep-tacting affinity chromatography columns in an AKTA FPLC system ...
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bioRxiv - Molecular Biology 2020Quote: ... as described before (Zelensky et al., 2017) at atmospheric oxygen concentration in media comprising 1:1 mixture of DMEM (Lonza BioWhittaker Cat ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Cell Biology 2023Quote: ... hydrogels were prepared by diluting collagen I stock to a final concentration of 2.5 mg/mL with 10% v/v 10x MEM-α (BE12-684F, Lonza), 10% v/v foetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... mES cells were grown on gelatinized dishes in (0.1% gelatin in water) as described before 77 at atmospheric oxygen concentration in media comprising 1:1 mixture of DMEM (Lonza BioWhittaker Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... buffer kit V (Lonza) and program A-023 ...
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bioRxiv - Cancer Biology 2021Quote: Mycoplasma Detection Kits (Lonza) and MycoSensor qPCR Assay Kits (Agilent Technologies ...