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Citations for Lonza :
1 - 50 of 208 citations for Dengue Virus serotype 3 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Virus was concentrated using ultracentrifugation (95000 g for 3 hr) and suspended using OPTI-MEM (Lonza). Viral transfections assays were performed by plating target 293T (ACE2+ve ...
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bioRxiv - Microbiology 2022Quote: ... The virus pellet was resuspended to EBM2 (Lonza) media ...
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bioRxiv - Microbiology 2022Quote: ... Virus was removed and 2 mL of overlay media (EMEM 1X Lonza 12-684F ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Molecular Biology 2020Quote: ... Limulus Amebocyte Lysate Assay (Lonza, Walkersville, MD, USA), 10% neutral buffered formalin (CellPath Ltd ...
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bioRxiv - Microbiology 2022Quote: ... All cells and virus stocks tested negative for mycoplasma using MycoAlert Plus reagents (Lonza). Heat-inactivated serum samples were serially diluted in 3-fold steps in DMEM supplemented with 2% heat-inactivated fetal bovine serum (HI-FBS) ...
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bioRxiv - Bioengineering 2022Quote: ... GM-3TM Lymphocyte Growth Medium-3 (LGM-3™) (Lonza), Opti-MEM Serum-Free medium (Thermofisher) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were loaded on 12% Prosieve 50 gels (Lonza) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded on 10% ProSieve gel (LONZA, LON50618), then subjected to electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: K562 cells stably expressing proinsulin [12] and HEK293T cells for virus production were cultured in IMDM (Lonza). All media were supplemented with 10% FCS ...
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bioRxiv - Microbiology 2020Quote: ... LPS level was determined using QCL-1000 Limulus amebocyte lysate system (Lonza) and found to be >5 pg/ μg of rfhSP-D.
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bioRxiv - Microbiology 2020Quote: ... All swabs were stored at −80°C in virus transport medium consisting of Minimum Essential Medium (MEM) – Eagle with Hank’s BSS and 25 mM Hepes (Lonza), glycerol 99% (Sigma Aldrich) ...
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bioRxiv - Genomics 2019Quote: ... The products were size selected on a 3% NuSieve 3:1 Agarose gel (Lonza), purified using NucleoSpin Gel and PCR clean-up columns ...
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bioRxiv - Genomics 2020Quote: ... donors (passage 2-3; Lonza) were continuously passaged to replicative exhaustion in complete Endopan-2 supplemented with 2% FBS under 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... The virus growth media was either Minimum Essential Medium – Eagle with Earle’s BSS (MEM Eagle EBSS) from Lonza (Rochester, NY) or Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2021Quote: Commercially available human primary PTs from 6 donors (3 males and 3 females, Lonza Walkersville Inc) were expanded at passage 4 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were co-incubated with virus and then supplemented with overlay medium that contains 1% SeaPlaque agarose (Lonza Bioscience, Basel, Switzerland). After 2 days of incubation ...
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bioRxiv - Biophysics 2021Quote: ... HUVECs (Lonza, expanded to passage 3) were transduced with GFP tagged VE-cadherin (65 ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 μg of pmaxGFP plasmid (Lonza) was added to transfection mixes to monitor transfection efficiency ...
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bioRxiv - Immunology 2020Quote: ... the endotoxin levels were measured via the QCL-1000 Limulus amoebocyte lysate system (Lonza) and found to be <5 pg/μg of rfhSP-D ...
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bioRxiv - Immunology 2021Quote: Endotoxin level in the AF was determined by Limulus amebocyte lysate assay (LAL; Lonza) according to the test procedure recommended by the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... T47D cells were transiently transfected with the avian tumor virus receptor A (TVA) by using electroporation (preset “FF150” program, 4D-Nucleofector transfection system; Lonza, Cologne, Germany). The cell suspension was cultured with full medium at 37 °C and 5% CO2 for 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... Endotoxin levels were evaluated by the Limulus amebocyte lysate assay (Endotoxin Testing Services, Lonza Europe), and the amount expressed was negligible ...
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bioRxiv - Genomics 2019Quote: ... The DNA fragments of 150–200 bp length were gel-extracted on 3% NuSieve 3:1 Agarose (Lonza). Both samples were sequenced by GATC Biotech AG (Konstanz ...
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bioRxiv - Bioengineering 2022Quote: ... and 3% FBS (all from Lonza Biosciences), termed astrocyte medium ...
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bioRxiv - Bioengineering 2021Quote: ... were used between passages 3-5 for paxillin experiments and culture medium was changed every 2-3 days (Lonza FBM Basal Medium supplemented with 2 v/v% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Dry powder (2-3 mg) was loaded into an HPMC size 3 capsule (VCaps® Plus, Lonza, Morristown, NJ). The capsule was placed in a Plastiape high resistance RS00 DPI that was then attached to a Next Generation Impactor (NGI ...
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bioRxiv - Microbiology 2021Quote: ... The levels of endotoxin were measured using the Limulus Amebocyte Lysate (LAL) Kinetic-QCL kit (Lonza). Phage or AAVP preparations containing endotoxin levels < 0.05 EU/mL were used in this study.
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... or either of two different gRNAs targeting exon 3 coding sequences of human TET2 (TET2-gRNA #1: 5’-TGGAGAAAGACGTAACTTC-3’ and TET2-gRNA #2: 5’-TCTGCCCTGAGGTATGCGAT-3’) were transfected with Nucleofector (Lonza). After transduction ...
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bioRxiv - Neuroscience 2023Quote: Larvae were anesthetized with 0.02% ethyl 3-aminobenzoate methanesulfonate salt (Tricane, (E10521, Ethyl 3-aminobenzoate methanesulfonate) and mounted in 1% low melting point agarose (50100, Lonza) in a 60 mm x 15 mm petri dish ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8%) ...
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bioRxiv - Immunology 2022Quote: ... supplemented with fetal calf serum (FCS, 3%, Lonza) and HEPES (1.8% ...
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bioRxiv - Cell Biology 2019Quote: ... and separated on a 3% MetaPhor gel (Lonza).
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bioRxiv - Microbiology 2023Quote: ... and 3 ml of ACK lysis buffer (Lonza) was added to the cells and incubated for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Neuroscience 2021Quote: ... embedded in 3% agarose (Lonza, # 50004, Rockland, ME, USA) and coronally sectioned (Leica VT1000S vibratome ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin was measured with the Limulus Amoebocyte Lysate (LAL) chromogenic end-point assay (Lonza®, Allendale, NJ, USA).
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Immunology 2021Quote: ... and the levels of endotoxins were measured using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). Fh12 was considered endotoxin free when endotoxin levels gave similar to background levels (<0.1 EU/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the absence of endotoxin was confirmed using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). nFhGST was concentrated by AMICON Ultra-15 Centrifugal filter unit and adjusted to 1 mg/ml as determined using the Pierce protein bicinchoninic acid (BCA ...
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bioRxiv - Cancer Biology 2019Quote: ... insulin and 3% fetal bovine serum (FBS) (Lonza, #CC-4123). MO3.13 ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were resolved on 3% Metaphor agarose gel (Lonza), and DNA fragments of sizes approximately 150-200 nt were isolated from the gel using Qiagen’s Gel Extraction Kit (Figure 1D) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were maintained in FGM-3 Medium (CC-4526, Lonza) and passaged at 80% confluence.
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bioRxiv - Microbiology 2022Quote: Sterile molten top NA (3 mL; 0.2 % SeaPlaque Agarose, Lonza) supplemented with CaCl2 and MgCl2 (both at 5 mM ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 3% agarose (SeaKem LE Agarose, Lonza). 150 μm thick vibratome sections (VT1000S vibratome ...
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bioRxiv - Neuroscience 2022Quote: ... brains were embedded in 3% agarose (SeaKem LE Agarose, Lonza) and vibratome-sectioned (VT1000S vibratome ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CF patient# 3 (#28388-0000450918, Lonza, male, 25 years-old) and four non-CF donors with no pathology reported ...
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bioRxiv - Systems Biology 2024Quote: ... and finally resuspended in a cold SSC cocktail (3× Lonza AccuGENE SSC ...
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bioRxiv - Genetics 2019Quote: ... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels, BioRad; or PAGEr EX Gels, Lonza; or home-made gels), proteins transferred onto PVDF membranes (Immobilon P ...