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Citations for Lonza :
1 - 39 of 39 citations for Cytomegalovirus gH Pentamer Complex since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: RNP complex was delivered using the Amaxa Nucleofector™ system (Lonza). Following trypsinization and washing with PBS ...
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bioRxiv - Genomics 2019Quote: ... Cells were nucleofected with RNP complex and donor vector using the Amaxa Nucleofector (Lonza VPH-1001). Clonal colonies were picked and genotyped by PCR followed by Sanger sequencing ...
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bioRxiv - Immunology 2019Quote: ... the ribonucleoprotein (RNP) complex was first formed by incubating 10μg of TrueCut Cas9 Protein V2 (Lonza) with 5μg of sgRNA for 10-30min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The resultant riboprotein complex was introduced into cells by nucleofection (4D-Nucleofector X unit, Lonza bioscience), using the SF cell line kit and A549 cell program (CM 130) ...
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bioRxiv - Immunology 2023Quote: ... a ribonucleoprotein (RNP) complex was first formed by incubating 5µg of TrueCut Cas9 Protein V2 (Lonza) with 10µg of sgRNA for 10 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA complex and Alt-R HDR Donor Oligos were transfected into HCT116 cells by electroporation (Lonza Nucleofector 96-well Shuttle System ...
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bioRxiv - Synthetic Biology 2019Quote: ... U2OS.eGFP.PEST cells were transfected with the RNP complex using the SE Cell Line 4D-Nucleofector kit (Lonza) following the pulse program of DN-130 ...
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bioRxiv - Cell Biology 2023Quote: ... Electroporation of the RNP complex was carried out using the SF Cell Line 4D-Nucleofector kit (Lonza) and program CM-130 with 2.0×105 cells ...
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bioRxiv - Cell Biology 2023Quote: ... gRNA/Cas9 RNP complex along with SOX10 donor template plasmid were nucleofected into 3450 iPSC (Lonza Biosciences). After nucleofection ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Immunology 2020Quote: ... 4 μl of the RNP-complex was added and transfected usine program CM150 of the nucleofection device (Lonza). After transfection cells were seeded in StemFlex™ medium (Thermo Fisher ...
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bioRxiv - Bioengineering 2023Quote: ... Electroporation of the RNP complex was performed using the Lonza 4D-Nucleofector (Lonza Group Ltd, Alpharetta, GA, USA) in P3 Primary Cell Solution with program DZ-100 ...
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bioRxiv - Cell Biology 2023Quote: ... The RNP complex was mixed with confluent 2i-grown ES cells re-suspended in P3 transfection buffer (Lonza) before being transferred to an electroporation microcuvette well (Lonza) ...
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bioRxiv - Immunology 2023Quote: ... 1×10e6 cells per guide were electroporated with the corresponding RNP complex using Lonza Electroporation Kit V (Lonza). After 48 h ...
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bioRxiv - Molecular Biology 2020Quote: ... 1×106 EBdCas9 cells were transfected with the above RNP complex and 100μM of ssDNA (HDR)(Sup Table 8) using AMAXA electroporation kit (LONZA) and plated in TeSR media (supplemented with 5μM ROCKi) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3uL of the total Cas9-RNP complex was added to 200,000 K562 cells resuspended in 18uL SF cell solution (Lonza) and subjected to nucleofection with the Lonza 4D nucleofectorsystem using manufactured specified conditions for K562 cells ...
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bioRxiv - Neuroscience 2023Quote: Electroporation with freshly assembled RNP complex was performed on hiPSCs using the Human Stem Cell Nucleofector Kit 1 (Lonza) in conjunction with the Amaxa Nucleofector 2b system (Lonza ...
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bioRxiv - Immunology 2023Quote: ... The precomplexed HDR-RNP complex was electroporated into primary human T cells as described above using P3 buffer (Lonza). The T cells were cultured in T cell media with 300 IU/ml IL-2 for 7-10 days ...
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bioRxiv - Immunology 2022Quote: ... IFNAR KO A549 cells were generated by CRISPR-Cas9 ribonucleoprotein (RNP) complex (IDT) transfection using the Nucleofector system (Lonza Bioscience). Specifically ...
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bioRxiv - Genetics 2022Quote: ... Cells were dissociated using accutase and 1 x 106 cells in P3 buffer were mixed with the RNP/template complex and nucleofected in large cuvettes (V4XP-3024 Lonza) using 4D-Nucleofector on program CA137 ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with EO-115 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 and Il15 medium were then activated by anti-human CD3/CD28 beads (Thermo Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 pmol of the dsODN was mixed together with 100 µl homemade nucleofection solution to the RNP complex and electroporated using Nucleofector 2b (Lonza) with A23 program and 2 mm electroporation cuvettes.
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bioRxiv - Immunology 2022Quote: ... 4.4 μl gRNA-Cas9 complex was added to a 20 μl cell suspension in P3 buffer and transferred into a nucleovette (Lonza), placed on Amaxa Nucleofector and 96 well Shuttle (Lonza ...
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bioRxiv - Immunology 2022Quote: ... containing RNP complex and enhancer DNA (IDT, Cat.1075915) and under electroporation with CM-137 program of 4D-nucleofactor X Unit (Lonza). Electroporated T cells recovered overnight in IL7 medium were then activated by anti-mouse CD3/CD28 beads (at 1:1 ratio for two days and expanded in culture medium containing 5ng/ml hIL2 ...
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bioRxiv - Immunology 2023Quote: CRISPR–Cas9 gene knockout was performed by transient Cas9/gRNA (RNP) complex electroporation using the P3 Primary Cell 4D-Nucleofector X Kit S (Lonza). On day 4 of culture ...
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bioRxiv - Genomics 2023Quote: ... 2.5e5 RPE1 CRISPRi cells were nucleofected with 3μM RNP complex using an SE Cell Line 4D X Kit S (Lonza Bioscience) on a 4D-Nucleofector (Lonza Bioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... were resuspended in 4D-Nucleofector Solution and then mixed with the Cas9/sgRNA complex and electroporated using the 4D-Nucleofector X Unit (Lonza). All tumor cell lines were maintained in high-glucose DMEM supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... were delivered as a ribonucleoprotein complex with the DNA donor template using a Nucleofector 2b Device and the Human Stem Cell Nucleofector Kit 1 (Lonza) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: CRISPR-mediated mutagenesis was carried out by transiently introducing CRISPR/Cas9 RNA-nucleoprotein complex into cultured NB4 cells with the 4D-Nucleofector system (Lonza). The sample preparation kit was Lonza SF Cell Line 4D-Nucleofector Kit S (Cat# V4XC-2024) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with RNP complex and HDR templates by nucleofection with SF Cell Line 4D-Nucleofector X Kit (Lonza) using 20-ml Nucleocuvette Strips ...
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bioRxiv - Microbiology 2023Quote: ... or 1×106 CEM-M7 Cas9 cells were transfected with the HiFi Cas9 Nuclease V3 (IDT)/gRNA complex (80 pmol/300 pmol) (Lonza) using a non-targeting or a GRN(5’-GCGATCCTGCTTCCAAAGATC-3’) ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed by pipetting with the full gRNA-Cas9 complex mix previously assembled then added in the electroporation chamber well (Lonza, V4XP3032). Cells were electroporated with the program EO-100 using the Lonza Nucleofector ...
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bioRxiv - Systems Biology 2024Quote: ... MT2 and MDA-MB-231 cells with HLA-A,B,C CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Three sgRNAs targeting HLA gene (HLA sgRNA#1 ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... MDA-MB-231 and PC3 cells with FBXO22 CRISPR-Cas9 knockout were generated through electroporation of Cas9-sgRNA ribonucleoprotein (RNP) complex using 4D-Nucleofector (Lonza Bioscience). Two sgRNAs targeting FBXO22 gene (FBXO22 sgRNA#1 ...
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bioRxiv - Physiology 2021Quote: ... were mixed with 1.49 μL of ssODN (100 μM) and Cas9 complex consisting of 18 μL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 μL of sgRNA (30 pmol/μL) ...
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bioRxiv - Cell Biology 2021Quote: Genes were knocked out using 1-2 synthetic sgRNAs (Synthego) in complex with spCas9 (Aldeveron 9214) that were electroporated into cells using a nucleofector system (Lonza V4XC- 1032) according to published methods (Hoellerbauer et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... were mixed with 1.49 µL of ssODN (100 µM) and Cas9 complex consisting of 18 µL SF 4D-nucleofector X solution + supplement1 (Lonza V4XC-2012), 6 µL of sgRNA (30 pmol/µL) ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then additionally nucleofected with ribonuclease complex of ITGB2 sgRNA and Cas9 using P3 Primary Cell 4D-Nucleofector™ X Kit S (Lonza, V4XP-3032) using 4D-Nucleofector (Lonza ...
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bioRxiv - Immunology 2023Quote: ... were incubated with sgRNA and CAS9 protein complex and electroporation was done using P3 Primary Cell 4D-NucleofectorTM X Kit L (Lonza, cat # V4XP-3024) and Lonza 4D nucleofector system ...