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Citations for Lonza :
1 - 50 of 3868 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were loaded on 12% Prosieve 50 gels (Lonza) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were loaded on 10% ProSieve gel (LONZA, LON50618), then subjected to electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... Lysate were pellet (500 g 8min) and red blood cells were lysate with 500 µl of ACK lysing buffer (Lonza) for 5min ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293 T-antigen (HEK293T) cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) and supplemented with 10% fetal bovine serum (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... HMLE cells were generated by introducing SV40 large T-antigen and human telomerase reverse transcriptase into a primary culture of normal mammary epithelial cells (Lonza). HMLE cells were cultured in MEGM ...
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bioRxiv - Molecular Biology 2020Quote: ... Limulus Amebocyte Lysate Assay (Lonza, Walkersville, MD, USA), 10% neutral buffered formalin (CellPath Ltd ...
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bioRxiv - Immunology 2024Quote: ... and HLA-A2+ T2 cells (can be efficiently loaded with exogenous peptides as they lack the transporter associated with antigen processing) were cultured in RPMI 1640 media (Lonza) supplemented with 10% FCS (Merck) ...
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bioRxiv - Microbiology 2020Quote: ... LPS level was determined using QCL-1000 Limulus amebocyte lysate system (Lonza) and found to be >5 pg/ μg of rfhSP-D.
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bioRxiv - Immunology 2021Quote: ... The frequency of cells producing IFN-γ in responses to antigen was quantified with ELISpot (Diaclone; 2B Scientific, Oxon, U.K.) performed in HL-1 serum-free medium (BioWhittaker; Lonza, Slough, U.K.), supplemented with L-glutamine and penicillin–streptomycin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... the endotoxin levels were measured via the QCL-1000 Limulus amoebocyte lysate system (Lonza) and found to be <5 pg/μg of rfhSP-D ...
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bioRxiv - Immunology 2021Quote: Endotoxin level in the AF was determined by Limulus amebocyte lysate assay (LAL; Lonza) according to the test procedure recommended by the manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Endotoxin levels were evaluated by the Limulus amebocyte lysate assay (Endotoxin Testing Services, Lonza Europe), and the amount expressed was negligible ...
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bioRxiv - Microbiology 2021Quote: ... The levels of endotoxin were measured using the Limulus Amebocyte Lysate (LAL) Kinetic-QCL kit (Lonza). Phage or AAVP preparations containing endotoxin levels < 0.05 EU/mL were used in this study.
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bioRxiv - Physiology 2022Quote: ... Cleared lysates were loaded into wells of the SDS-agarose gels (1% SeaKem Gold Agarose [Lonza, Basel ...
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bioRxiv - Cell Biology 2019Quote: ... Endotoxin was measured with the Limulus Amoebocyte Lysate (LAL) chromogenic end-point assay (Lonza®, Allendale, NJ, USA).
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bioRxiv - Immunology 2019Quote: ... Endotoxin concentrations were measured in all protein samples using the Limulus Amebocyte Lysate Kit – QCL-1000 (Lonza, Basel, Switzerland). The rMIC1 ...
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bioRxiv - Immunology 2021Quote: ... and the levels of endotoxins were measured using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). Fh12 was considered endotoxin free when endotoxin levels gave similar to background levels (<0.1 EU/ml) ...
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bioRxiv - Microbiology 2023Quote: ... and the absence of endotoxin was confirmed using the Chromogenic Limulus Amebocyte Lysate QCL-1000 Assay (Lonza, Walkersville, MD). nFhGST was concentrated by AMICON Ultra-15 Centrifugal filter unit and adjusted to 1 mg/ml as determined using the Pierce protein bicinchoninic acid (BCA ...
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bioRxiv - Genetics 2019Quote: ... Lysates were resolved by electrophoresis (Mini-PROTEAN TGX Precast Gels, BioRad; or PAGEr EX Gels, Lonza; or home-made gels), proteins transferred onto PVDF membranes (Immobilon P ...
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bioRxiv - Immunology 2020Quote: ... Endotoxin concentrations ranged from 150-175 EU/mL as determined using the limulus amebocyte lysate assay (Lonza, Walkersville, MD, USA). This concentration of ODE has been previously shown to produce optimal experimental effects and is well-tolerated in mice (11 ...
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bioRxiv - Cell Biology 2020Quote: Microspheres were tested for LPS contamination using the Limulus Amebocyte Lysate (LAL) QCL-1000™ kit (Lonza Walkersville, Inc., Olten, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Residual endotoxins in the purified phage preparations were determined by the Limulus Amebocyte Lysate (LAL) Kinetic-QCL Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was determined to be more than 99% by sodium dodecyl sulfate and polyacrylamide gel (SDS-PAGE) with an endotoxin concentration lower than 2 units/mg protein measured by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza). In previous experiments we demonstrated that these recombinant proteins were functionally devoid of significant endotoxin contamination ...
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bioRxiv - Immunology 2019Quote: ... Mucin proteins was prepared as previously described45 and confirmed to be endotoxin free using a Limulus Amebocyte Lysate (LAL) assay kit (Lonza, USA). Full details of mucin protein purification and preparation are shown in supplementary methods ...
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bioRxiv - Immunology 2023Quote: ... acetylated chitin oligomers were suspended in endotoxin free water and tested for endotoxin level by using the limulus amebocyte lysate (LAL) assay (Lonza, CH). Levels below 0.25 EU/ml (<25 pg/ml LPS ...
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bioRxiv - Immunology 2023Quote: ... The levels of endotoxin in the purified protein samples were assessed using the QCL-1000 Limulus amebocyte lysate system (Lonza, Basel, Switzerland), and it was found that the recombinant proteins had endotoxin levels of ∼ 4 pg/µg ...
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bioRxiv - Pathology 2020Quote: ... The purity of the final recombinant proteins was more than 99% with an endotoxin concentration lower than 2 units/mg protein by Limulus Amebocyte Lysate PYROGENT™ 125 Plus (Lonza; Walkersville, MD).
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bioRxiv - Cell Biology 2021Quote: ... endotoxin-free phosphate buffered saline (PBS) and verified to be free of detectable lipopolysaccharide contamination by Limulus lysate assay (Lonza, Walkersville, Maryland, USA), MCSF (10 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: Endothelial cells: Human umbilical vein endothelial cells (HUVECs, Lonza) in passages 4-8 were cultured in EGM2-MV medium (Lonza).
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bioRxiv - Immunology 2019Quote: ... cells rested overnight in complete medium (human cells) or X-Vivo 15™ (mouse cells) (Lonza) were activated with IL-2 (100U/ml ...
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Weak Membrane Interactions Allow Rheb to Activate mTORC1 Signaling Without Major Lysosome EnrichmentbioRxiv - Cell Biology 2019Quote: ... Hela cells (750,000 cells) were electroporated (Cell Line Kit V, electroporation program Q01; Lonza, Basel, Switzerland) with 10 μl RNP complex crRNA and single-stranded DNA (ssDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... Pelleted cells were treated with red cell lysis buffer (Lonza) for two minutes to eliminate red blood cells.
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bioRxiv - Immunology 2022Quote: ... Cells were nucleofected using Cell Line Nucleofector Kit V (Lonza) for cell lines and P3 Primary Cell 4D-Nucleofector X Kit L (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... Endothelial cells were maintained in Endothelial Cell Basal Media (Lonza) supplemented with the complete Endothelial cell singlequot nutrients (Lonza) ...
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bioRxiv - Biochemistry 2023Quote: ... HUVEC cells were cultured in Endothelial Cell Growth Medium (Lonza). All other media were purchased from Wako and supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... HepaRG cells (hepatic cell line purchased from Lonza, Colmar, France) were grown in William’s E medium (Gibco ...
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bioRxiv - Immunology 2021Quote: HUVEC cells (Lonza) were cultivated in endothelial cell medium (EGM-2 ...
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bioRxiv - Immunology 2020Quote: HEK293 cells (Lonza) were cultured in DMEM (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: 293FT cells (Lonza) were transfected at 70-80% confluence in 10 cm dishes with 3 μg pKLV2 library plasmid ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated tumor cells were lysed red blood cells (10-548E, Lonza) and blocked Fc receptors with anti-CD16/32 (101319 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were suspended in Mesenchymal stem cell growth media (MSCGM, Lonza) and seeded on a culture plate ...
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bioRxiv - Pathology 2021Quote: ... Both cells were maintained in EMG2 Endothelial Cells Growth Media (Lonza) and used for experiments between P2 and P5 ...
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bioRxiv - Microbiology 2023Quote: ... and NHBE cells (normal human broncial epithelial cells, Lonza #CC-2540S). A variant of MDCK-SIAT1 overexpressing influenza NS1 from A/PR8/1934 was generated using a lentiviral vector as previously described ...
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bioRxiv - Cell Biology 2023Quote: ... into HAP1 cells (1.5 × 106 cells) by Nucleofector Kit V (Lonza) with a Nucleofector device (Lonza ...
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bioRxiv - Molecular Biology 2023Quote: ... K562 CRISPRi cells were nucleofected (SF Cell Line 4D kit; Lonza) with the RNP ...
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bioRxiv - Biochemistry 2023Quote: ... Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were resuspended in 240μl P3 Primary Cell Solution (Lonza). 15μl Alt-R Cas9 enhancer (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... Melanoma cells were resuspended at a cell density of 50,000 cells in 20 µL SF electroporation buffer with supplement (Lonza) and TILs were resuspended at a cell density of 1 million cells in 20 µL P3 electroporation buffer with supplement (Lonza ...
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bioRxiv - Neuroscience 2022Quote: ... Each well contained a single-cell suspension of 1.6 × 105 cells in 20 µl of Primary Cell P3 buffer with supplement (Lonza) containing 2 µg Cas9 ...
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bioRxiv - Immunology 2019Quote: ... washed once in PBS and resuspended in Mouse B cell Nucleofector Solution with Supplement (murine B cells) or Primary Cell Nucleofector Solution 3 with Supplement (human B cells) prepared to the manufacturer’s instructions (Lonza) at a concentration of 4 - 5 × 106 cells / 86 μL ...