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Citations for Lonza :
2601 - 2650 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... perturb-seq vectors by electroporation using the Amaxa 4D Nucleofector system (Lonza) with buffer P3 and pulse code EO-115 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Nucleofector 2b device (LONZA, Basel, Switzerland), following manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) and were used with or without addition of drugs and LPS (0.5 ug/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... MVECs were expanded and grown on 1% gelatin-coated dishes in endothelial basal medium-2 (EBM-2 (Lonza; 3156) with 10 % heat-inactivated FBS (Gibco) ...
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bioRxiv - Genomics 2023Quote: ... and 20 μg of pDC2 plasmid with the required cloned guide DNA and electroporated using an Amaxa 4D-Nucleofactor (Lonza), using program FP158 as previously described45 ...
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bioRxiv - Genomics 2023Quote: ... purified Percoll-enriched mature schizonts (∼20 μl packed volume) were suspended in 100 μl of P3 primary cell solution (Lonza) containing 60 μg of linearized repair plasmid DNA (repair plasmid 1 and 2 separately in two separate transfection experiments ...
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bioRxiv - Bioengineering 2023Quote: ... were expanded to passage 6 or 7 in endothelial growth media (EGM-2 BulletKit, Lonza), and culture medium was changed every other day ...
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bioRxiv - Bioengineering 2023Quote: ... filtered through a 40-µm cell strainer and layered on LymphoPrep™ (Lonza) with a density centrifugation at 2200 RPM (800 G ...
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bioRxiv - Bioengineering 2023Quote: PDLSCs mineralization on the scaffolds after 21 days of osteogenic differentiation was assessed using OsteoImage Mineralization Assay (Lonza). This assay’s staining reagent binds specifically to the hydroxyapatite portion of bonelike nodules deposited by cells ...
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bioRxiv - Cell Biology 2023Quote: ... Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit, Lonza, Catalog #: V4XP-3024) were mixed with 7 μL of RNP and 12 μg of single-stranded oligodeoxynucleotide (ssODN ...
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bioRxiv - Cell Biology 2023Quote: ... The RNP was incubated at room temperature for 20 mins prior to electroporation with the 4D-Nucleofector System (Lonza). Next 1 × 106 cells resuspended in 93 μL of P3 Primary Cell Nucleofector Solution (P3 Primary Cell 4D-Nucleofector X Kit ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged by differential trypsinization using a Trypsin/EDTA reagent pack (LONZA). Viability and cell count were assessed using the trypan-blue method.
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bioRxiv - Cell Biology 2023Quote: ... Pre-warmed D-PBS (Lonza) was added to the apical side of the insert 5min prior to TEER measurement ...
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bioRxiv - Cell Biology 2023Quote: HBE cells were previously established and expanded in standard BEGM Bulletkit (BEGM; Lonza). Passage 1 BEGM-expanded HBE cells were subsequently added to collagen I coated flasks and co-cultured with an equal amount of irradiated NIH/3T3 feeder cells in F-media containing the ROCK inhibitor Y-27632 as described previously(5 ...
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bioRxiv - Cell Biology 2023Quote: ... and tested for mycoplasma (LT07-318, Lonza). All cells were maintained at 37°C in a 5% CO2 incubator.
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bioRxiv - Biophysics 2023Quote: ... 1 mM sodium pyruvate (Lonza 13-115E), and 100 U/mL penicillin-streptomycin was used for imaging.
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bioRxiv - Cell Biology 2023Quote: ... and MycoZap™ Plus-CL (VZA-2012; Lonza). They were kept at 37°C in a humidified 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2023Quote: Cryopreserved primary human hepatocytes (Lonza #HUCPG) were cultured according to the supplier’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Hepatocytes were thawed at 37°C and placed in warmed thawing medium (Lonza # MCHT50P), resuspended in Hepatocyte Plating Medium (Lonza #MP100) ...
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bioRxiv - Cell Biology 2023Quote: ... resuspended in Hepatocyte Plating Medium (Lonza #MP100), plated in collagen-coated 96 well plates at 50,000 cells/well ...
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bioRxiv - Cell Biology 2023Quote: ... we assessed cell death (ToxiLight assay, Lonza, LT07-117) and cell viability (MUH cell viability assay [2]).
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bioRxiv - Cell Biology 2023Quote: ... Cells were centrifuged and the supernatant was aspirated and pellet was resuspended in the appropriate volume of P3 supplement buffer (20μl per condition by mixing 16.4μl P3 buffer and 3.6μl of supplement 1, Lonza, V4XP-3032). The cells were transferred into 16-well nucleovette strips (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were transferred into 16-well nucleovette strips (Lonza). After 10 minutes at room temperature electroporation was performed on the Amaxa 4D Nucleofector (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... After 10 minutes at room temperature electroporation was performed on the Amaxa 4D Nucleofector (Lonza) with the DS-138 custom program unless otherwise stated ...
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bioRxiv - Cell Biology 2023Quote: ... Five million BJAB or OCI-LY1 cells were transfected with 2 μg plasmid DNA using the SF Cell Line 4D-Nucleofector X Kit L (Lonza) and the AMAXA Nucleofector biosystem (BJAB ...
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bioRxiv - Cell Biology 2023Quote: Schneider S2 cells were grown in Schneider’s medium (21720001, Gibco or 04-351Q, Lonza) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
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bioRxiv - Developmental Biology 2023Quote: ... using Vectashield® mounting medium (Vectorlabs, Newark, CA, USA) and embedded in 1% low melting agarose (Lonza, Basel, Switzerland). Images were obtained using a SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Developmental Biology 2023Quote: ... and were separated by electrophoresis in 1-1.2% (w/v) agarose gel (Lonza 50004) supplemented with 0.03μl/ml DNA staining dye (Serva ...
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bioRxiv - Developmental Biology 2023Quote: ... and co-transfected with an ssODN donor template containing the desired modification into H9/WA09 cells via nucleofection (Amaxa P3 primary cell 4D nucleofector X kit L, Lonza) using the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2023Quote: The 96-well flat bottom plates were coated with 30µl of 1.2% agarose solution (1.2% w/v of Lonza Bioscience 50004 agarose added to deionised water and sent for autoclaving ...
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bioRxiv - Developmental Biology 2023Quote: ... using pMAX-GFP plasmid (Lonza) as template ...
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bioRxiv - Cancer Biology 2023Quote: ... which was generated by adding necessary supplements (Lonza; CC-3166) to Prostate Epithelial Basal Media (PrEBM ...
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bioRxiv - Cancer Biology 2023Quote: ... to Prostate Epithelial Basal Media (PrEBM, Lonza; CC-4177), as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: All cell lines were authenticated by Brca1/2-specific PCR-based genotyping (mouse)7,8 and they were regularly tested for mycoplasma contamination (Mycoalert, Lonza).
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bioRxiv - Cancer Biology 2023Quote: ... HUVEC cells were maintained in growth factor-supplemented media (EBM + EGM-2 aliquots + 10% FBS) from Lonza (C-3121, CC-4542). MDA-MB-231 cell line was modified to express GFP (green fluorescent protein ...
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bioRxiv - Cancer Biology 2023Quote: ... Co-expression plasmids were delivered by nucleofection into U2OS DSB reporter cell lines using the SE Cell Line 4D-Nucleofector XL Kit (Lonza Biosciences). To induce a DSB ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were mycoplasma negative (MycoAlert kit, Lonza). Experiments were performed using charcoal-stripped serum (CTS) ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection via electroporation used a Nucleofector 2b (Lonza), following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... high glucose (4.5 g/L) with L-glutamine (BE12-604F, Lonza) supplemented with 10% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid was used to transiently transfect HeLa-CCL2 cells using the Amaxa system (Lonza) with Solution R and Program I-013 ...
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bioRxiv - Cancer Biology 2023Quote: ... a plasmid mix was prepared consisting of 1uL of Electroporation Enhancer (100uM, Lonza), 1.5 uL of 2ug/uL ABE8e-NRCH ribonucleoprotein editor,83 1uL of base editor primer (50uM stock ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 uL of 2ug/uL ABE8e-NRCH ribonucleoprotein editor,83 1uL of base editor primer (50uM stock) and 3.6uL of Nucleofector supplement (Lonza). The ABE8e-NRCH base editor was a kind gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in 15uL of Nucleofector solution from the P3 kit (Lonza) in a 1.5mL microcentrifuge tube ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lines were routinely verified via STR genotyping and tested for mycoplasma contamination using the Lonza MycoAlert assay (Lonza). Below is a list of cell line details:
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... using 0.6% low-melting-point agarose (Lonza) containing 0.014% tricaine ...
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bioRxiv - Cancer Biology 2023Quote: ... Electroporation protocol for each cell line was identified using pmaxCloningTM vector (Lonza, # VDC-1040). The optimal protocol for each cell line is indicated here below:
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bioRxiv - Immunology 2023Quote: HEK-293T (ATCC; ATCC®CRL-11268™) were cultured in Iscove’s modified Dulbecco’s medium (IMDM) (Lonza, Basel, Switzerland) supplemented with 10% FBS (Labtech ...
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bioRxiv - Immunology 2023Quote: ... NALM6 (DSMZ; ACC 128) lines were cultured in complete RPMI (RPMI-1640, Lonza, Basel, Switzerland) supplemented with 10% FBS and 2 mM GlutaMAX (Gibco ...
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bioRxiv - Immunology 2023Quote: Low passage primary human bronchial epithelial cells (HBEC) from healthy adult donors were obtained commercially (Lonza, Walkersville, MD, USA) and cultured at air-liquid interface according to the manufacturer’s instructions (Stem Cell Technologies ...