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Citations for Lonza :
9251 - 9300 of 9615 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... were obtained from Lonza (CC-2581) and maintained for no more than 8 passages in Smooth Muscle Cell Growth Media (SM-GM2, Lonza) supplemented with 5% FBS and 1X Penicillin/Streptomycin (Gibco) ...
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bioRxiv - Molecular Biology 2019Quote: ... Endothelial cells were maintained in Endothelial Cell Basal Media (Lonza) supplemented with the complete Endothelial cell singlequot nutrients (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with the complete Endothelial cell singlequot nutrients (Lonza). H1299 cells were maintained in RPMI containing 10% FBS (Hyclone ...
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bioRxiv - Immunology 2019Quote: Bone marrow was extracted from femurs and tibias isolated from BALB/cJ or C57BL/6J mice and cultured in DMEM media (Lonza, Basel, Switzerland) with 10% heat-inactivated fetal bovine serum (Corning Inc. ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of transposon and transposase vectors was performed on day 2 with 0,5 µg transposon MC and 2 µg SB100X transposase RNA per 1×106 T cells on a 4D-Nucleofector according to the manufacturer’s instructions (Lonza). Transfected CD8+ and CD4+ T cells were expanded using the G-Rex culture system (Wilson-Wolf) ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was transiently transfected into HEK SNAP-β2 β-arr-KO cells by electroporation with the Amaxa Nucleofector II device (Lonza). The included program Q-001 is designed for high expression levels in HEK cells and was used according to the manufacture’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... E14 cells (5 × 106) were transfected with 10 μg of the appropriate plasmid with the Mouse ES Cell Nucleofector™ Kit (Lonza) and allowed to grow for 48h after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... Dr Keydar and were cultured in RPMI-1640 medium (Lonza) supplemented with 10% FCS (Life technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... in Grace’s insect media (Lonza, Walkersville, MD, USA)) into a 9-well glass plate using forceps ...
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bioRxiv - Cell Biology 2019Quote: Approximately 5 whole ovary pairs were dissected in room temperature Grace’s insect media (Lonza, Walkersville ...
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bioRxiv - Bioengineering 2019Quote: ... harvested by 0.25% trypsin/EDTA and resuspended in 100 μL nucleofector solution (Amaxa Biosystems/Lonza, Cologne, Germany) containing 5 μg CRISPR/Cas9 plasmid ...
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bioRxiv - Bioengineering 2019Quote: ... then electroporated by the program A-033 with Nucleofector 2b Device (Amaxa Biosystems/Lonza, Cologne, Germany). Afterward ...
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bioRxiv - Cell Biology 2019Quote: ... Ascorbic Acid and 5% fetal bovine serum (Lonza Cat. no. CC-3202). At this time the medium supplying the epithelial channel was switched to differentiation medium ...
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bioRxiv - Cell Biology 2019Quote: ... All cell lines were tested regularly to ensure that they were mycoplasma free (MycoAlert, Lonza). For immunofluorescence ...
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bioRxiv - Genomics 2019Quote: ... cell viability was determined by trypan blue (Lonza) exclusion using a hemocytometer (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... were obtained from the laboratory of Stephen Elledge at Harvard Medical School (Solimini et al., 2012) and cultured in MEGM Mammary Epithelial Growth Medium (Lonza CC-3150). To reduce background fluorescence in imaging experiments we used the same medium without phenol red (Lonza CC-3153 basal medium supplemented with growth factors and other components from the CC-4136 kit) ...
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bioRxiv - Cell Biology 2019Quote: ... To reduce background fluorescence in imaging experiments we used the same medium without phenol red (Lonza CC-3153 basal medium supplemented with growth factors and other components from the CC-4136 kit) ...
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bioRxiv - Genomics 2019Quote: ... The iPSCs were passaged using Versene (Lonza) from one well into three wells of a 6-well plate ...
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bioRxiv - Genetics 2019Quote: ... major WT and Δprmt7 using Amaxa Nucleofector IIb (Lonza). Transfectants were selected on 1% agar M199 plates with 10 μg/mL G418 and PCR-validated using 5’FLR and 3’FLR primers (Supplementary Figures S2B and C).
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bioRxiv - Synthetic Biology 2019Quote: ... and 1mM sodium pyruvate (Lonza). A polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2019Quote: ... For CENP-C overexpression we transfected HeLa cells with pEGFP-CENP-C using the Amaxa Cell Line Nucleofector Kit R (Lonza cat#VVCA-1001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Special medium was provided by Lonza (EGM™-2 MV Microvascular Endothelial Cell Growth Medium mixed with supplements according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... the dissociated neurons were centrifuged down to remove the supernatant and resuspended in 80 to 100 μl of Amaxa electroporation buffer for mouse neurons (Lonza Cologne GmbH; Cologne, Germany) with mixtures of siRNA (0.2 nmol per transfection ...
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bioRxiv - Microbiology 2019Quote: ... plasmid DNA (50 µg per pool) was resuspended in 100 µl of buffer P3 (Lonza) with 4 µl ATP (625 mM) ...
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bioRxiv - Molecular Biology 2019Quote: ... basal HBECs were washed in HBSS (Lonza, CC-5034), trypsinized (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... trypsinized (Lonza, CC-5034) and 50.000 cells were resuspended in 500ul BEGM in wells of 24 well plates ...
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bioRxiv - Molecular Biology 2019Quote: ... basal HBECs were washed in HBSS (Lonza, CC-5034), trypsinized (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... trypsinized (Lonza, CC-5034) and 200.000 cells were resuspended in 8.6μl P3 Primary Cell Nucleofector Solution (Lonza ...
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bioRxiv - Molecular Biology 2019Quote: ... and 200.000 cells were resuspended in 8.6μl P3 Primary Cell Nucleofector Solution (Lonza, V4XP-3032). The cell solution was then mixed with the RNP solution ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to a well of a 16-well Nucleocuvette Strip (Lonza, V4XP-3032) and electroporated using the CM-113 program on the Amaxa 4D Nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... and electroporated using the CM-113 program on the Amaxa 4D Nucleofector (Lonza). Electroporated cells were then swiftly transferred to 6 well plates containing 2mL pre-warmed BEGM and incubated at 37°C.
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bioRxiv - Molecular Biology 2019Quote: Basal cells were trypsinized (Lonza, CC-5034) and stained with anti-human-MHCI-APC (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: Human umbilical vein endothelial cells (HUVECs) were cultured in EBM-2 media supplemented with the SingleQuotsTM kit (Lonza, Slough, UK). 3T3 fibroblasts were cultured in high glucose DMEM (invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... using the Amaxa nucleofector system (Lonza) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... conjugated to FITC or GFP siRNA (300 nM) conjugated to FITC two days prior to adoptive T cell therapy using the Mouse T cell Nucleofector™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: ... and a guide RNA targeting Tp53 (Wang et al., 2015) were transfected into cells using a Nucleofector 2b Device (Lonza) with the setting T023 ...
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bioRxiv - Cell Biology 2019Quote: HUVECs (Lonza) were cultured in bovine hypothalamus extract ...
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bioRxiv - Immunology 2019Quote: ... Leishmania tarentolae cells were transfected via electroporation (Nucleofector™ 2b Device, AmaxaTM Human T Cell Nucleofector™ Kit, Lonza, Basel, Switzerland) with the respective expression cassette containing the selective antibiotic marker Nourseothricin (NTC ...
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bioRxiv - Genomics 2019Quote: ... were free of mycoplasma and routinely tested using the MycoAlert Detection Kit (Lonza).
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bioRxiv - Genomics 2019Quote: ... were free of mycoplasma and routinely tested using the MycoAlert Detection Kit (Lonza).
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bioRxiv - Genomics 2019Quote: ... These hiPSC stocks were confirmed to be Mycoplasma negative (MycoAlert Detection Kit, Lonza), and proved euploid by conventional G-banding karyotyping (Diagnostic Cytogenetics Inc ...
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bioRxiv - Microbiology 2019Quote: ... Cell pellets were resuspended in nucleofector buffer (Amaxa Mouse Macrophage Nucleofector Kit (Lonza)) and 2ug of siRNA was added to each tube (Dharmacon) ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transferred to a cuvette and nucleofected in the Nucleofector 2b Device (Lonza) under Y-001 program settings ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were authenticated as recommended by AACR (http://aacrjournals.org/content/cell-line-authentication-information) and tested for the absence of mycoplasma using MycoAlert® (Lonza, Basel, Switzerland) or MycoFluor (Invitrogen ...
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bioRxiv - Bioengineering 2019Quote: K562 cells were transfected with two gRNAs targeting exon 1 and 3 of NGLY1 and Cas9 plasmid (lentiCas9-Blast) by Nucleofection according to the manufacturer’s protocol (Nucleofector, Lonza). LentiGuide-Puro (Addgene plasmid #52963 ...
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bioRxiv - Cell Biology 2019Quote: ... U2OS and RPE1 cells were transfected using Cell Line Nucleofector Kit V and a Nucleofector electroporator (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Stably transfected U2OS cells were generated using using Cell Line Nucleofector Kit V and a Nucleofector electroporator (Lonza). After 24 hours ...
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bioRxiv - Immunology 2019Quote: ... Primary cells were grown in complete bronchial epithelial cell growth medium (BEGM)(Lonza) with growth factor supplements in submerged monolayer culture and then seeded at 2 x 105 cells/mL in transwells until confluent ...