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Citations for Lonza :
751 - 800 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... as a control two days prior to adoptive T cell therapy using the P3 Primary Cell 4D-Nucleofector X™ Kit (Lonza Bioscience) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and the GFP-K17ΔNLS (Hobbs et al., 2015) were nucleofected into HeLa cells using SE Cell Line 4D X nucleofector Kit S (Lonza #V4XC-1032) with setting DS-138 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... plasmids pSpCas9n(BB)-ZEB2-Guide-A &-B (0.5 µg of each plasmid) were electroporated into 1×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation ...
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bioRxiv - Immunology 2021Quote: ... gRNA_pX335 plasmids were transfected into EL4 cells by nucleofection using an Amaxa nucleofector and the Cell Line Nucleofector Kit L (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UCB-MNC were thawed and cultured at 2 million cells/mL in X-VIVO 15 serum-free cell-culture medium (Lonza, Basel, Switzerland) supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) under the atmosphere containing 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were seeded onto 96-well plates at a density of 3×104 cells per well in 100 μL cell culture media that consisted of DMEM (Lonza, Basel, Switzerland), 1% penicillin–streptomycin (Lonza ...
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bioRxiv - Synthetic Biology 2022Quote: ... human aortic endothelial cells (CD31+) from were maintained in EGM™-2 Endothelial Cell Growth Medium-2 Bullet Kit™ (Cat # CC-3162; Lonza) and EGM™ −2 MV Microvascular Endothelial Cell Growth Medium-2 Bullet KitTM (Cat #CC-3202 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then passaged as previously described and 8×105 cells were resuspended in 100 µl of nucleofector solution (Lonza, VPH-5012). Depending on the experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... CRISPR-CAS9 knockout in SU-DIPGXIII cells was performed using the Amaxa P3 Primary Cell 4D-Nucleofector X Kit L (Lonza, V4XP-3012). First ...
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bioRxiv - Cancer Biology 2024Quote: Fresh single cell suspension remaining from PDOTS processing (S3) was grown in SmGM-2 Smooth Muscle Cell Growth Medium (Lonza, CC-3181) supplemented with SmGM-2 SingleQuots Supplement Pack (Lonza ...
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bioRxiv - Microbiology 2024Quote: ... Non-adherent cells were centrifuged again and resuspended in 2 mL of bronchial epithelial cell growth medium (BEGM) (Lonza cat# CC-3170) supplemented with Primocin (InvivoGen ...
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bioRxiv - Genomics 2024Quote: ... were complexed for 20 minutes at room temperature and were nucleofected into 5E5 PEmax parental cells using the SE Cell Line 4D-Nucleofector X Kit (Lonza V4XC-1032) and program FF-120 ...
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bioRxiv - Developmental Biology 2024Quote: ... cell pellets of 200x103 cells were resuspended with 20 µL of supplemented nucleofector solution P3 Primary Cell 4D-Nucleofector® S (Lonza, V4XP3032) with indicated amounts of multi-guide sgRNAs (Synthego ...
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bioRxiv - Cell Biology 2024Quote: ... and sgRNAs (see Key Resources Table) were delivered by nucleofection to a single cell suspension of 105 iBCs in Primary Cell P3 solution (Lonza, #V4XP-3032). Cells were re-cultured and the resulting clonally-derived spheroids were isolated ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow was harvested from 8–12-week-old female B6 or BALB/c mice and processed into a single cell suspension and red blood cells were lysed with ACK lysis buffer (Lonza, Walkersville, MD). NK cells were isolated by magnetic cell separation with a NK cell isolation kit and an AutoMACS Pro (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... puromycin containing medium were changed and every 4 days cells were fed EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™ (LONZA) supplemented with GlutaMax (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... and BEBM™ Bronchial Epithelial Cell Growth Basal Medium supplemented with the BEGM™ Bronchial Epithelial Cell Growth Medium Single Quots™ Supplements and Growth Factors (Lonza). Fresh media were added to the cells every 2-3 days until the confluent monolayer of mesothelial cells was obtained (typically between 5-10 days post-isolation).
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bioRxiv - Neuroscience 2023Quote: ... 8 x 105 hiPSCs in single-cell suspension were nucleofected with 5 μg SpCas9-sgRNA plasmid using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, #V4XP-3024) in combination with the 4D Nucleofector Unit X (Lonza ...
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bioRxiv - Developmental Biology 2023Quote: ... ePB master vector and helper (Plasmid AW-27) were nucleofected into the established SOX2::mCitrine cell line using the P3 Primary Cell 4D-Nucleofector X Kit (Lonza; V4Xp-3012). G-418 (40ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... MV411 cells were electroporated with Cas9/sgRNA complexes targeting the HDR insertion using a Lonza SF Cell Line 4D Nucleofector (Lonza V4XC-2032). RNP complexes were formed by mixing 8.5 μg of TrueCut Cas9 Protein v2 (Invitrogen A36499 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were electroporated at 5 million cells/100 mL cells per cuvette using a Lonza 4-D nucleofector with pulsecode DP-148 (Lonza, VVPA-1002). Cells were co-cultured for 5 additional days with human astrocyte before transferring cells to homeostatic culture conditions (MGdM media ...
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bioRxiv - Biophysics 2023Quote: ... The cells were maintained in a 100 mm cell culture dish using SmGM2 Smooth Muscle Cell Growth Medium BulletKit (SmGM2, CC-3182, Lonza, Basel, Switzerland) at 37 °C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... cells then were electroporated using electroporation code EH-100 and using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, V4SP-3096). Knockout pools were maintained for an additional nine days prior to coculturing with H80 feeder cell line with IL-2 (Final conc 20 U/mL ...
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bioRxiv - Immunology 2023Quote: ... Purified templates together with 2 µg of LentiGuide-Gbp-Chr3-sg3+sg4 were electroporated into RAW-Cas9 cells using Lonza SF cell line X kit (Lonza, V4XC-2012) on a Lonza 4D-Nucleofector unit (Lonza) ...
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bioRxiv - Cell Biology 2023Quote: PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent.
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bioRxiv - Pharmacology and Toxicology 2023Quote: HUVEC cells were grown at 37o C in the Endothelial cell basal medium-2 (LONZA #Cat No-CC-3156 and CC-4176) and were used with or without addition of drugs and LPS (0.5 ug/ml ...
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bioRxiv - Genomics 2023Quote: ... Each plasmid library was transfected into K562 or A549 cells by electroporation using Lonza SF Cell Line 4D-Nucleofector X Kit (Lonza V4XC-2012) with Lonza 4D-Nucleofector ...
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bioRxiv - Bioengineering 2023Quote: ... MCF-10A cells were cultured using an MEGM™ Mammary Epithelial Cell Growth Medium BulletKit™ without gentamycin-amphotericin B mix (Lonza) following ATCC’s instructions ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... and immediately transfected by Nucleofection into HT-1080 cells using the Amaxa SF Cell Line 4D-Nucleofector X kit S (Lonza, PBC2-00675) and either program FF-113 (HT1080 ...
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bioRxiv - Bioengineering 2023Quote: ... 100,000 cells were resuspended in 20μl P3 reagent of the P3 Primary Cell 4D-Nucleofector® X Kit S (Lonza V4XP-3032). 1 μg total plasmid was used for a single nucleofection event and nucleofected by program EH-100 ...
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bioRxiv - Immunology 2023Quote: ... Constructs were transiently transfected into KRT17 null A431 cells using the SF Cell Line 4D-Nucleofector™ X Kit (Lonza #V4XC-2032) and Lonza 4D-nucleofector X unit “A431 cell” program ...
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bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected into 106 U2OS cells using the Amaxa Nucleofector System with the Cell Line Nucleofector Kit V (catalog no: VCA-1003, Lonza, Cologne, Germany) utilizing program X-001 according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...
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bioRxiv - Cell Biology 2024Quote: The Human Umbilical Vein Endothelial Cells wild type, Pooled (HUVECs) cell line was obtained from Zhao Lab (MTU, MI) and was originally purchased from Lonza (cat#: C2519A). The cells were used from passages 5-9 for the experiments and were cultured in endothelial cell growth Medium-2 (EGM™-2 ...
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bioRxiv - Cell Biology 2024Quote: ... An amount of 106 cells were resuspended in 100 µl of SE Cell Line Solution (Cat. No. V4XC-2024, Lonza, Basel, Switzerland) containing 3.5 µg of the respective plasmid and electroprated with pulse DG113 ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Neuroscience 2021Quote: ... H9 cells were nucleofected using the Amaxa nucleofector (Lonza) with the P3 primary cell 4D nucleofector kit L (Lonza LONV4XP-3024) ...
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bioRxiv - Biophysics 2020Quote: Primary human mammary epithelial cells (HMECs; Lonza, Basel, Switzerland) were cultured in T-75 flasks in mammary epithelial basal medium (MEGM basal medium CC-3151 ...
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bioRxiv - Cancer Biology 2021Quote: ... Melanoma cell lines were cultured in RPMI-1640 (Lonza) containing 5% FBS (Sigma) ...
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PD-1 checkpoint blockade disrupts CD4 T cell regulated adaptive B cell tolerance to foreign antigensbioRxiv - Immunology 2021Quote: ... After lysing with ACK red cell lysis buffer (Lonza) and passed through a 70 µm cell strainer (Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... The construct was nucleofected into hTERT-RPE1 cells (Lonza) and sorted by flow cytometry into single cells in a 24 well plate ...
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bioRxiv - Cell Biology 2020Quote: ... Single-cell suspensions were resuspended in SAGM medium (Lonza) supplemented with 1% FBS (see Table S5 for details) ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were maintained in DMEM (Lonza, #12-604F) supplemented with 10% FCS (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with surface antibodies in PBS (Lonza) + 2% FCS ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in P3 Primary Cell nucleofector solution (Lonza) to a density of 107 cells / 100 uL ...
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bioRxiv - Molecular Biology 2021Quote: ... Min6 cells were transfected using an Amaxa Nucleofector (Lonza) protocol G-016 according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... All Sf9 cells were grown in insect-EXPRESS (Lonza) media ...
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bioRxiv - Molecular Biology 2020Quote: ... HeLa cells were grown in MEM (Lonza, Basel, Switzerland) and all other cell lines in DMEM (Corning Inc ...