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Citations for Active Motif :
1 - 50 of 168 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Global DNA methylation was studied using the global DNA methylation assay kit - LINE-1 (Active Motif) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... A Global DNA Methylation Assay Kit (Cat#55017, Active Motif, USA) was used for DNA methylation analysis ...
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bioRxiv - Physiology 2022Quote: ... The activity of NF-κB in binding DNA was assessed in nuclear fractions by a DNA Binding TransAM NF-κBp65 Assay (Active Motif).
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bioRxiv - Cell Biology 2022Quote: NF-κB DNA-binding activity was measured using the TransAM NF-κB activity assay (Active Motif). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif). The samples were run in duplicates and absorbance was measured using a spectrophotometer at 450 nm.
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bioRxiv - Cancer Biology 2021Quote: ... The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: CUT&Tag assays were performed using CUT&Tag-IT assay kit (Active Motif) on 50,000 CD34+ cells according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was eluted using DNA purification elution buffer (Active motif). The eluted DNA was used in qPCR using primers targeting putative cohesin-bound positions in the integration site (coordinates ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: NRF2-ARE binding assay was performed with an ELISA-based TransAM NRF2 Assay Kit (Active Motif, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Chromatin immunoprecipitation (ChIP) assays were conducted using the ChIP-IT EXPRESS assay kit protocol (Active Motif, Carlsbad, CA). After exposure to Aβo ...
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bioRxiv - Cell Biology 2022Quote: CUT&Tag-IT assay kit (Active Motif) was used on 3,000 HSC according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: ... and the CUT&Tag-IT Assay Kit (Active Motif) following the manufacture’s manual ...
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bioRxiv - Genetics 2019Quote: ... proteinase K and DNA purified using the ChIP DNA Purification kit (Active Motif #58002) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... myelin basic protein (Active Motif, 31314) was employed as a substrate for recombinant CDKL5 ...
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bioRxiv - Cell Biology 2020Quote: RelA and IRF-3 transcription factor activation assays (Active Motif) were carried out using the same protocol with the only difference being the 96-well plates and the antibodies used ...
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bioRxiv - Genomics 2022Quote: ... at 0.125 μg/assay or anti-H3K27me3 (39155, Active Motif) at 0.5 μg/assay ...
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bioRxiv - Cancer Biology 2023Quote: Oligonucleotide-binding assay (TransAM kit, Active Motif, Carlsbad, CA, USA) was used to detect nuclear Sp1 in the tumor tissue ...
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bioRxiv - Cancer Biology 2024Quote: ... and ChIP-Seq assays were then performed by Active Motif Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8μM of purified DNMT1 protein (Active Motif) was incubated with 30000 cpm of 32P-labelled aptamers and single or double-stranded ...
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bioRxiv - Cell Biology 2023Quote: The recombinant proteins p53 (#81091, Active Motif), PITPα (#ab101666 ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Cancer Biology 2020Quote: The DNA-binding activity of NF-κB was quantified by enzyme-linked immunosorbent assay using the TransAM® NF-κB family activation assay kit (Active Motif North America, Carlsbad, CA) to specifically detect and quantify the DNA-binding capacity of the NF-κB subunits p65 ...
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bioRxiv - Cell Biology 2021Quote: ChIP assays were performed using the ChIP-IT Kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase activity was measured using the LightSwitch Luciferase Assay Reagent (Active Motif) on a SpectraMax M5 plate reader (Molecular Devices ...
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bioRxiv - Genomics 2019Quote: ... ChIP-seq assays were conducted with antibodies against H2A.Z (39647, Active Motif) and MYC-tag Mouse (2276 ...
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bioRxiv - Cancer Biology 2020Quote: SDHD 3’UTR LightSwitch Luciferase Reporter Assay was purchased from Active Motif. Cells were co-transfected with 30 nM miRNA and 100 ng reporter using Lipofectamine 3000 in a 96-well plate ...
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bioRxiv - Genomics 2020Quote: ... and assay was performed with Nuclear Complex Co-IP Kit (Active Motif) as instructed using antibodies against HA-tag (Thermo Fisher cat#26183) ...
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bioRxiv - Plant Biology 2019Quote: ... ChIP-seq assays were conducted with antibodies against H2A.Z (39647, Active Motif), H3K4me3 (04-745 ...
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bioRxiv - Neuroscience 2019Quote: ... ChIP assays were then conducted using antisera to CTCF (61311, Active Motif), histone H3K27ac (39133 ...
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bioRxiv - Neuroscience 2020Quote: ChIP assays were performed using the ChIP-IT Express Kit (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant CDKL5 and myelin basic protein (Active Motif, 31314) as a substrate were incubated in a kinase buffer (Cell Signaling ...
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bioRxiv - Neuroscience 2021Quote: The Next Gen DNA Library Kit (Active Motif) includes molecular identifiers (MIDs) ...
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bioRxiv - Cell Biology 2023Quote: ... The Methylated DNA Immunoprecipitation (MeDIP) Kit (Active Motif) was used according to the instructions by the manufacturer with some modifications ...
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bioRxiv - Genetics 2019Quote: ... Urinary albumin was measured using the Albumin Blue Fluorescent Assay Kit (Active Motif), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ChIP-qPCR assays were performed using the ChIP-IT Express Kit (Active Motif) as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ChIP assays were performed with a commercial kit (Chip-IT kit, Active motif). The cortices from P2 wildtype mice were minced and crosslinked in 1% formaldehyde (F8775 ...
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bioRxiv - Molecular Biology 2019Quote: ... antibody was used for H3 ChIP assays and active motif 39140 (Active Motif) was used for pan-acetyl H3 ChIP assays.
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bioRxiv - Cancer Biology 2020Quote: The capture antibody for the Nu.Q™ H3.1 assay (Active Motif, Carlsbad, CA) was validated for use in canines using Mass Spectrometery by Spectrus Corp (Beverly ...
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bioRxiv - Neuroscience 2022Quote: ChIP assays were performed with ChIP-IT High Sensitivity Kit (Active Motif, USA). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ChIP assays were performed by using a Chromatin Immunoprecipitation (ChIP) kit (Active Motif). The MYCN-amplified cell line NGP and MYCN-non-amplified cell line SK-N-AS were cross-linked with 1% formaldehyde ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...