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Citations for Active Motif :
1 - 50 of 118 citations for Protein A G Magnetic Beads for IP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2020Quote: Co-IP was done using magnetic beads Co-IP kit (Universal Magnetic CO-IP Kit, Active Motif, 54001-am) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Immune complexes were collected using 20 μL of protein G magnetic beads (Active Motif). After elution from the beads ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: Co-IP was performed using a Universal Magnetic Co-IP kit (Active Motif). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads (Active Motif) were then washed once with 1x PBS-Tween and combined with the antibody-lysate mixture ...
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bioRxiv - Genomics 2023Quote: ... Magnetic beads (Active Motif) were then washed once with 1× PBS-Tween and combined with the antibody-lysate mixture ...
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bioRxiv - Microbiology 2020Quote: ... including Protein A magnetic beads as per manufacturer’s instructions (Active Motif, USA). The input and immunoprecipitated DNA were quantified by real-time PCR using a Stratagene MX3500P PCR System ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... using magnetic beads (Active Motif) diluted in commercial ChIP-buffer (Active Motif ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Physiology 2024Quote: ... The universal magnetic Co-IP kit (Active motif, 54002, Carlsbad, CA, USA) was used and the appropriate antibodies (specified in table 2 ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was pre-cleared using 30 μl protein G agarose beads (Active Motif) pre-blocked with BSA per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p, Active Motif for Pol II Ser2p and Ab817 for total Pol II ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitated DNA fragments were collected by magnetic beads (Active Motif), purified ...
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bioRxiv - Immunology 2019Quote: ... Then beads were incubated with H3K27ac antibody (1 µg per IP, rabbit, Active Motif, 39133) or normal rabbit IgG (1 µg per IP ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear protein fractions were prepared by using Nuclear Complex co-IP kit (Active Motif #5400), according to the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear proteins were extracted using the Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... the immunoprecipitated samples (now bound to the beads) were washed four times with Co-IP wash buffer (Active Motif). The washed immunoprecipitated samples bound to beads were then resuspended in 20 μl of 2X Reducing Loading Buffer (130 mM Tris pH 6.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear co-IP was performed using the Nuclear Co-IP kit (Active Motif) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Co-Immunoprecipitation (Co-IP) was performed using the Co-IP kit (Active Motif) according to the manufacturer’s instructions and as described previously (33) ...
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bioRxiv - Cancer Biology 2024Quote: Co-immunoprecipitation (co-IP) was performed using the Nuclear Complex Co-IP Kit (Active Motif). 6T1 Smarcd1 OE cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells per dish ...
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bioRxiv - Pathology 2022Quote: Co-immunoprecipitation (Co-IP) was conducted using the Nuclear Complex Co-IP Kit (Active Motif, 54001) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: Co-immunoprecipitation (Co-IP) was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) and Dynabeads™ Protein G for Immunoprecipitation according to manufacturer’s protocol (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Nuclear complex Co-IP kit (Active Motif) was used for the Co-Immunoprecipitation according to manufacturer’s instructions for most protein interactions ...
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bioRxiv - Genomics 2023Quote: ... H3K27me3 (Active motif Cat#: 39055, 3μl/IP), H3K36me3 (Abcam Cat#ab9050 ...
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bioRxiv - Cancer Biology 2021Quote: ... or Nuclear Complex Co-IP Kit (Active Motif). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... the Nuclear Complex Co-IP Kit (Active Motif) was used according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: The Nuclear Complex Co-IP Kit (Active Motif) was utilized for co-immunoprecipitation on nuclear lysates ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... in moderate stringency buffer (IP High buffer [Active Motif] and Protease inhibitor cocktail [Sigma] ...
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bioRxiv - Molecular Biology 2021Quote: ... or the Chromatin IP DNA Purification Kit (Active Motif). The following primary antibodies were used for ChIP experiments ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... antibodies following the manufacturer’s protocol (Active motif Co-IP kit). Immunoprecipitated products were examined by SDS-PAGE-Western blot with the appropriate antibodies (7074 ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were transferred to a new tube with 50 μl Protein-G Dynabeads (Active Motif) and further incubated for 1 hr before following the manufacturer’s recommendations for washing of the beads and library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... A chromatin IP DNA Purification Kit (Cat No 58002 Active Motif) was used to purify the samples after CHIP before PCR was performed using Myh11 primers [5’-CCC TCC CTT TGC TAA ACA CA - 3’ and 5’ - CCA GAT CCT GGG TCC TTA CA – 3] ...
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bioRxiv - Cell Biology 2021Quote: ... A chromatin IP DNA Purification Kit (Cat No 58002 Active Motif) was used to purify the samples after CHIP before PCR was performed using Myh11 promoter primers [5’ -CCC TCC CTT TGC TAA ACA CA – 3′ and 5’ – CCA GAT CCT GGG TCC TTA CA – 3] as previously published [79] ...
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bioRxiv - Genomics 2020Quote: ... and assay was performed with Nuclear Complex Co-IP Kit (Active Motif) as instructed using antibodies against HA-tag (Thermo Fisher cat#26183) ...
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bioRxiv - Molecular Biology 2019Quote: ... IP was performed using ChIP-IT high sensitivity kit (53040, Active Motif) with some modifications ...
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bioRxiv - Developmental Biology 2019Quote: ... IPs were performed using the ChIP-IT high sensitivity kit (Active Motif). IP and input samples were treated with RNase A followed by proteinase K ...
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bioRxiv - Molecular Biology 2021Quote: ... Coupled antibody-Dynabeads were prepared by adding Dynabeads protein A/G and 2 μg antibody [H3K9ac (Active Motif) or H3K27ac (Abcam)] to 100 μl ice-cold dilution buffer and rotating the tubes end-over-end at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Cancer Biology 2021Quote: Co-immunoprecipitiation was performed using the Nuclear Complex Co-IP Kit (Active Motif). 4T1 cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells/dish ...
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bioRxiv - Cell Biology 2020Quote: Co-IP was performed using following antibodies: EZH2 (Active Motif, 39933, 1:100), Pol2-S2p (Active Motif ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif); 2 μg of each extract was loaded for each experiment and histone H3 was examined as loading control ...