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Citations for Active Motif :
1 - 50 of 65 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... in moderate stringency buffer (IP High buffer [Active Motif] and Protease inhibitor cocktail [Sigma] ...
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bioRxiv - Developmental Biology 2019Quote: ... lysed in RIPA buffer or Prep Buffer (ChIP-IT, Active Motif) and sonicated in Diagenode Bioruptor Pico ...
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bioRxiv - Cell Biology 2023Quote: ... A neutralization buffer (Active Motif) containing 0.1M DTT (Millipore Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... diluted in commercial ChIP-buffer (Active Motif) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and Histone Extraction Buffer (Active Motif #37513) containing Halt’s protease/phosphatase inhibitor cocktail (Thermo-Fisher #78440 ...
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bioRxiv - Cancer Biology 2020Quote: ... The following day the beads were washed once with ChIP Buffer I and twice with ChIP Buffer II (Active Motif) before elution with 50mM Glycine (pH 2.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP Buffer I (Active Motif, La Hulpe, Belgium), 1X PIC and water ...
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bioRxiv - Cell Biology 2021Quote: ... and buffer C1 (Active Motif, Cat. No. 37484). After incubating on ice for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Nuclei were resuspended in shearing buffer (Active Motif) pulse sonicated using a Sonics Vibra Cell CV18 sonicator fitted with a micro-probe at 25% amplitude for 15 min on ice using 30 sec on/off cycles ...
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bioRxiv - Molecular Biology 2019Quote: ... Isolated cells were picked in 1 μl of 1× PBS and transferred into 4.4 μl of lysing buffer containing 4 μl mTRAP™ Lysis Buffer (Active Motif) and 0.4 μl (10 ng ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in ChIP lysis buffer (Active Motif) supplemented with protease inhibitors and incubated on ice for 30 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were extracted in Complete Lysis Buffer (Active Motif AM1 Lysis buffer containing 1 mM DTT and protease inhibitors) ...
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bioRxiv - Developmental Biology 2023Quote: ... Fixation was stopped using Stop buffer by Active Motif and incubating for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was eluted using DNA purification elution buffer (Active motif). The eluted DNA was used in qPCR using primers targeting putative cohesin-bound positions in the integration site (coordinates ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were resuspended in 100ul chromatin sheering buffer (Active Motif) and placed on 96 well plate sealed with PCR film (MiniAMP Optical Adhesive Film ...
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bioRxiv - Immunology 2019Quote: ... To each sonicated sample 300 µL ChIP Buffer (Active Motif) supplemented with PMSF (Carl Roth) ...
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bioRxiv - Neuroscience 2023Quote: ... nuclei pellet was washed once with 1 mL Nuclei Wash Buffer and then once with 1 mL of 1X CUT&Tag Wash Buffer (from Active Motif CUT&Tag IT Assay kit). Remove CUT&Tag Wash Buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed five times with AM1 Wash Buffer (Active Motif) then DNA eluted as per ChIP-IT kit instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in hypotonic lysis buffer (Nuclear Extract Kit, Active Motif). Cytosolic and nuclear fraction were separated by centrifugation (14,000 × g for 10 min at 4 °C) ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with Wash Buffer AM1 (Active Motif). ChIP DNA was eluted in 100 μl Elution Buffer AM4 (Active buffer) ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 µl of EasyPep lysis buffer containing 1X deacetylase inhibitors (Active Motif) and 40 ng/µl trypsin/LysC and 50 µl each of reducing and alkylation solutions (provided with the EaspPep kit ...
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bioRxiv - Immunology 2019Quote: Cytoplasmic extracts were generated by disrupting cells using hypotonic buffer (Active Motif, Carlsbad, CA) and pelleting the cell debris by centrifugation at 14000 × g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... precleared lysates were adjusted to 1X high stringency IP buffer (Active Motif, cat# 37510) with protease/phosphatase inhibitors ...
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bioRxiv - Bioengineering 2021Quote: ... Immunostaining was performed by diluting primary antibody in MAXbind Staining buffer (Active Motif, #15253), and incubating tissue samples in the antibody solution at 37°C for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... then resuspended in 50 μl of complete lysis buffer (Active Motif, Carlsbad, CA, USA). Pellets were shaken at 4°C for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed using 100 µl of ice-cold ATAC lysis buffer (Active Motif), followed immediately by addition of 1 ml pre-chilled wash buffer (10 mM Tris-HCl pH 7.4 ...
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bioRxiv - Bioengineering 2021Quote: ... we prepared antibody solutions by diluting primary antibody in MAXbind Staining buffer (Active Motif, #15253) and performed post-decrowding immunostaining (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... and tagmented using the enzyme and buffer provided in the ATAC-Seq Kit (Active Motif). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2021Quote: ... PCR duplicated alignments were removed from the BAM files using a perl script by Active Motif. Finally ...
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bioRxiv - Genetics 2023Quote: ... and nuclei were resuspended in 50 μl transposition mix (25 μl 2× transposition buffer (Active Motif), 10 μl transposase (Active Motif) ...
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bioRxiv - Cancer Biology 2024Quote: ... Pellets were then resuspended in the appropriate volume of ice-cold histone extraction buffer (Active Motif) and incubated overnight at 4°C on a rotator ...
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bioRxiv - Neuroscience 2021Quote: ... 5 microliters of the insoluble fraction were then diluted in Complete Lysis Buffer (Active Motif; Carlsbad, CA) and nuclear protein concentration was determined using a Bradford assay (Bio-rad ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in 100 µl cold Antibody Buffer and 1 µl antibody (TET1, Active Motif #61943) was added for incubating on nutator overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix ...
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bioRxiv - Genetics 2020Quote: ... were pre-blocked in fresh blocking buffer (0.5% BSA in PBS) and incubated with 2.5 µL of polyclonal H3K9me3 antibody (AB_2532132, Active Motif) or Rabbit IgG (negative control ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were Polytron minced and homogenized by douncing in Prep Buffer (ChIP-IT High Sensitivity Kit, Active Motif). They were then sonicated in 100 µl of sonication buffer (0.1%SDS ...
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bioRxiv - Biochemistry 2019Quote: ... at 4°C with Active Motif enzymatic shearing cocktail in 100 µl Complete Digestion Buffer (both from Active Motif), and centrifuged for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 times with cold PBS and resuspended in ChIP buffer supplemented with PIC (Protease Inhibitor Cocktail; Active Motif) and PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Immunology 2023Quote: ... The chromatin in sonication buffer was sheared to 200-400bp by sonication using an EpiShear Probe Sonicator (Active Motif) at 30% amplitude ...
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bioRxiv - Cancer Biology 2024Quote: ... the immunoprecipitated samples (now bound to the beads) were washed four times with Co-IP wash buffer (Active Motif). The washed immunoprecipitated samples bound to beads were then resuspended in 20 μl of 2X Reducing Loading Buffer (130 mM Tris pH 6.8 ...
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bioRxiv - Cell Biology 2020Quote: Controls and bleomycin-injured MLE12 cells were lysed in ice-cold buffer (PBS, 0.05% Tween 20, pH 7.4) containing protease inhibitors (Active motif, 37491) and phosphatase inhibitor (Active motif ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were washed once with ice cold PBS and lysed in 130μL Sonication Buffer (10mM Tris-HCl pH 8, 0.25% SDS, 2mM EDTA, 1x protease inhibitor cocktail (Active Motif # 37490), 1mM PMSF) ...
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bioRxiv - Genomics 2019Quote: ... and 1x protease inhibitor) before being resuspended in 50 μL of Buffer 2 containing 0.5 μL antibody (H3K27ac from Active Motif, 39122 ...
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bioRxiv - Cancer Biology 2022Quote: ... were washed twice in 1X IP Low buffer supplemented with protease inhibitor and PMSF following manufacturer’s guidelines (Active Motif, 37511). 200µl of nuclear extracts were incubated with the GFP-Trap beads for 1hour at 4°C ...
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bioRxiv - Cell Biology 2020Quote: Fibroblast cells were lysed in ice cold buffer (PBS, 0.05% Tween 20, pH 7.4) containing protease inhibitors (Active motif, 37491) and phosphatase inhibitor (Active motif ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041; H3K27Ac, Active Motif #39133 ...
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bioRxiv - Molecular Biology 2019Quote: ... the supernatant (nuclear extract) was transferred to a new tube and the pellet (chromatin) was resuspended in 350 μl digestion buffer (Active Motif, ChIP-IT Enzymatic Kit ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were blocked using LI-COR blocking buffer for 1 hour followed by overnight incubation with 1:50 mouse H4K20me3 (39672, Active Motif) at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... A portion of the frozen tumor excised from mice was thawed on ice and homogenized in Complete Lysis Buffer (Active Motif) for whole lysate extraction Protein concentrations were determined using Bradford reagent ...