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Citations for Active Motif :
1 - 50 of 108 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Microbiology 2023Quote: ... Nuclear proteins were isolated according to the manufacturer instruction (Active Motif nuclear extraction). Nrf2 TransAM ELISA-kit (Active Motif ...
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bioRxiv - Physiology 2019Quote: ... Ishikawa cell nuclear and cytoplasmic protein fractions were extracted using Nuclear Extraction Kit (Active Motif, Belgium) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The nuclear extraction was performed using the Nuclear Extract kit (Active Motif) according with manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... GSCs (10 million cells / Co-IP) were collected and prepared for nuclear extraction and purification using a nuclear extraction kit (Active Motif). Nuclear extracts were treated with an enzymatic shearing Cocktail for DNA digestion to release undissociated protein complexes ...
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bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear and cytoplasmic protein lysates were prepared using the Nuclear Extract Kit (Active Motif) or Nuclear Complex Co-IP Kit (Active Motif) ...
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bioRxiv - Molecular Biology 2022Quote: ... Nuclear protein fractions were prepared by using Nuclear Complex co-IP kit (Active Motif #5400), according to the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear proteins were extracted using the Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: Nuclear proteins were prepared using fibroblasts at 80-90% confluency and the Nuclear Extract Kit (Active Motif), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... cells were harvested and nuclear protein complex lysates were prepared using the Nuclear Complex Co-IP Kit (Active Motif). Co-immunoprecipitation with two biological replicates was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial proteins were extracted using Nuclear Extract Kit (Active Motif, Germany) according to manufacturer instructions ...
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bioRxiv - Physiology 2022Quote: ... protein from a subset of muscles was fractionated into nuclear and cytoplasmic components using a Nuclear Extract Kit (Active Motif; 40010) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear extracts were prepared using a nuclear extract preparation kit (Active Motif) and were added to the wells of a 96-well microtiter plate ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear lysates were prepared using the Nuclear Extracts Kit (Active Motif, # 40010). 20-50 ug Proteins were separated by SDS-PAGE and transferred into nitrocellulose membrane using the iBlot Transfer Stacks and the iBlot2 transfer system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear and cytoplasmic fractionation was performed with Nuclear Extract Kit (40010, Active Motif) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif); 2 μg of each extract was loaded for each experiment and histone H3 was examined as loading control ...
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bioRxiv - Cancer Biology 2022Quote: Nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif) and 10 μg of each extract was loaded to examine protein levels of SMYD3 ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear co-IP was performed using the Nuclear Co-IP kit (Active Motif) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear or cytosolic fractions were prepared using a Nuclear Extract Kit (Active Motif, USA).
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bioRxiv - Cancer Biology 2020Quote: Nuclear and cytoplasmic fractionation was performed with the Nuclear Extract Kit (Active Motif, 40010). Cells collected from 15 cm cell culture plates were centrifuged and resuspended in 500 µL 1X Hypotonic buffer and incubated for 5 min on ice ...
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bioRxiv - Immunology 2023Quote: ... and nuclear fraction was acquired using Nuclear Complex co-IP kit (Active Motif, 54001), which allows a gentle release of DNA-bound protein complexes in the nucleus ...
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bioRxiv - Cancer Biology 2024Quote: 4T1 nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... and nuclear lysates were prepared using the Nuclear Complex Co-IP kit (Active Motif).
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bioRxiv - Cancer Biology 2020Quote: Nuclear extracts from Jurkat cells were prepared using a Nuclear Extract Kit (40010, Active Motif), according to manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: ... Harvest and isolation of nuclear and cytoplasmic fractions were performed using Nuclear Extract Kit (Active Motif). Samples were quantified and immunoblotted as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear fraction digestion and collection was performed using Nuclear Complex Co-IP Kit (Active Motif, 54001). 25µl per IP of GFP-Trap Magnetic Agarose beads (Chromotek ...
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bioRxiv - Neuroscience 2019Quote: ... and nuclear histone (H3K27Ac, 39133, Active Motif) which were used to visualize cell populations in the immunocytochemical analysis ...
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bioRxiv - Cancer Biology 2023Quote: Nuclear complex Co-IP kit (Active Motif) was used for the Co-Immunoprecipitation according to manufacturer’s instructions for most protein interactions ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Cancer Biology 2021Quote: ... or Nuclear Complex Co-IP Kit (Active Motif). Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... the Nuclear Complex Co-IP Kit (Active Motif) was used according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: The Nuclear Complex Co-IP Kit (Active Motif) was utilized for co-immunoprecipitation on nuclear lysates ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in hypotonic lysis buffer (Nuclear Extract Kit, Active Motif). Cytosolic and nuclear fraction were separated by centrifugation (14,000 × g for 10 min at 4 °C) ...
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bioRxiv - Genomics 2020Quote: ... and assay was performed with Nuclear Complex Co-IP Kit (Active Motif) as instructed using antibodies against HA-tag (Thermo Fisher cat#26183) ...
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bioRxiv - Cancer Biology 2021Quote: Co-immunoprecipitiation was performed using the Nuclear Complex Co-IP Kit (Active Motif). 4T1 cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells/dish ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblast cell nuclear fraction was extracted using a commercially available kit (Active motif, 40010) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: ... and nuclear co-IP was performed according to the manufacturer’s protocol (Active Motif, 54001). 25 x 106 cells were used per IP reaction ...
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bioRxiv - Molecular Biology 2023Quote: Subcellular fractionation was performed using the Nuclear Complex Co-IP kit (Active Motif, USA) according to manufacturer’s protocol until isolation of the nucleus ...
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bioRxiv - Cell Biology 2024Quote: ... and Histone Extraction Buffer (Active Motif #37513) containing Halt’s protease/phosphatase inhibitor cocktail (Thermo-Fisher #78440 ...
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bioRxiv - Genetics 2019Quote: ... containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta (CEBPB ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear and cytoplasmic RNA separation assay was conducted using RNA Subcellular Isolation Kit (Active motif), according to previously detailed method(61) ...
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bioRxiv - Cancer Biology 2024Quote: Co-immunoprecipitation (co-IP) was performed using the Nuclear Complex Co-IP Kit (Active Motif). 6T1 Smarcd1 OE cells were seeded onto 15 cm tissue culture dishes at a seeding density of 5×106 cells per dish ...
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bioRxiv - Biochemistry 2019Quote: Nuclei were purified using the Active Motif Nuclear Complex Co-IP Kit (Active Motif, Carlsbad, CA) as described in the manufacturer’s manual (www.activemotif.com/catalog/25/nuclear-complex-co-ip-kit) ...
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bioRxiv - Cancer Biology 2019Quote: Cytoplasmic and nuclear RNA were isolated and purified using the RNA Subcellular Isolation Kit (Active Motif) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: Co-immunoprecipitation (Co-IP) was conducted using the Nuclear Complex Co-IP Kit (Active Motif, 54001) according to the manufacturer’s manual ...