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Citations for Active Motif :
1 - 33 of 33 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear extracts were prepared using a nuclear extract preparation kit (Active Motif) and were added to the wells of a 96-well microtiter plate ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear extracts from Jurkat cells were prepared using a Nuclear Extract Kit (40010, Active Motif), according to manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... and cytosolic extracts from NES cells were prepared by using the Nuclear Extract Kit (Active Motif) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and nuclear extracts were isolated using the reagents and protocol provided in the Active Motif Nuclear Extract Kit (Active Motif). IL-13 effects on NFκB p52 DNA binding was evaluated using the TransAM NFκB Transcription Factor Assay Kit (Active Motif) ...
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bioRxiv - Immunology 2020Quote: ... Nuclear extract from HuT78 cells (endogenous SP140) was prepared following manufacturer’s protocol (Active Motif nuclear extract and co-IP kit). Protein concentration was determined by Bradford Assay (Pierce) ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial proteins were extracted using Nuclear Extract Kit (Active Motif, Germany) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and resuspended in hypotonic lysis buffer (Nuclear Extract Kit, Active Motif). Cytosolic and nuclear fraction were separated by centrifugation (14,000 × g for 10 min at 4 °C) ...
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bioRxiv - Genetics 2020Quote: ... Chr13.1-cl and Chr13.2-cl KO ES cells was determined by native ChIP-seq and anti-H3K9me3 serum (Active Motif, #39161) as described previously (45) ...
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bioRxiv - Immunology 2020Quote: ... The nuclear extraction was performed using the Nuclear Extract kit (Active Motif) according with manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear lysates were prepared using the Nuclear Extracts Kit (Active Motif, # 40010). 20-50 ug Proteins were separated by SDS-PAGE and transferred into nitrocellulose membrane using the iBlot Transfer Stacks and the iBlot2 transfer system (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear and cytoplasmic fractionation was performed with Nuclear Extract Kit (40010, Active Motif) according to the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif); 2 μg of each extract was loaded for each experiment and histone H3 was examined as loading control ...
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bioRxiv - Cancer Biology 2022Quote: Nuclear extracts were prepared using the Nuclear Complex Co-IP kit (Active Motif) and 10 μg of each extract was loaded to examine protein levels of SMYD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear or cytosolic fractions were prepared using a Nuclear Extract Kit (Active Motif, USA).
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bioRxiv - Immunology 2019Quote: Cytoplasmic extracts were generated by disrupting cells using hypotonic buffer (Active Motif, Carlsbad, CA) and pelleting the cell debris by centrifugation at 14000 × g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Nuclear and cytoplasmic protein lysates were prepared using the Nuclear Extract Kit (Active Motif) or Nuclear Complex Co-IP Kit (Active Motif) ...
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bioRxiv - Cancer Biology 2020Quote: Nuclear and cytoplasmic fractionation was performed with the Nuclear Extract Kit (Active Motif, 40010). Cells collected from 15 cm cell culture plates were centrifuged and resuspended in 500 µL 1X Hypotonic buffer and incubated for 5 min on ice ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta (CEBPB ...
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bioRxiv - Molecular Biology 2020Quote: ... Harvest and isolation of nuclear and cytoplasmic fractions were performed using Nuclear Extract Kit (Active Motif). Samples were quantified and immunoblotted as described above ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Nuclear proteins were isolated from RAW264 cells using Nuclear Extract Kit (Active Motif, Carlsbad, CA, USA) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: Nuclear proteins were prepared using fibroblasts at 80-90% confluency and the Nuclear Extract Kit (Active Motif), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: ... fractionation of MDA-MB-231 and 4T1 cells was first performed using the Nuclear Extract kit (Active Motif). The level of protein binding to DNA consensus sequence was then assessed by ELISA using the TransAM NF-kB Family kit (Active Motif ...
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bioRxiv - Cell Biology 2021Quote: Cytoplasm and nucleus of keratinocytes were separated by using Nuclear Extract Kit (Active Motif, Inc., Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Nrf2 activity was quantified using nuclear extracts and a TransAm NRF2 activity kit from Active Motif (cat# 50296) following the manufacturers included instructions.
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... 1 mg of total HEK293T cellular extract was incubated with 5 μg of anti-SET8 antibody (Active Motif, 61009), anti-LSF antibody (Millipore,17-10252) ...
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bioRxiv - Synthetic Biology 2022Quote: The custom rabbit polyclonal antiserum against PARP-1 was generated in-house by using purified recombinant amino-terminal half of PARP-1 as an antigen (now available Active Motif; cat. no. 39559). The custom recombinant antibody-like anti-poly-ADP-ribose binding reagent (anti-PAR ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected after 24 hours of stimulation and performed with cell fractionation using Nuclear Extract Kit (Active Motif 40410). For flow cytometry analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... the supernatant (nuclear extract) was transferred to a new tube and the pellet (chromatin) was resuspended in 350 μl digestion buffer (Active Motif, ChIP-IT Enzymatic Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Physiology 2022Quote: ... protein from a subset of muscles was fractionated into nuclear and cytoplasmic components using a Nuclear Extract Kit (Active Motif; 40010) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Cell Biology 2019Quote: Isolation of nuclear protein extracts from ATI-like cells after 6 days of culture was performed with a Nuclear Extract Kit according to the manufacturer’s instructions (Active Motif, 40010, La Hulpe, Belgium) as described (Hofmann et al. ...