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Citations for Active Motif :
1 - 50 of 387 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 1 (Active Motif, #71001), Human Negative Control Primer Set 3 (Active Motif ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-lamin-A/C (1:5000, Active Motif, #39287), rabbit anti-Emerin (1:5000 ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human histone H3 (Active Motif, Cat. 61799), 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins produced in vitro were subjected to interaction assays for 2 hours at 4°C under mild rotation with Drosophila nuclear extracts or recombinant human CTD (Active Motif). Bound proteins were washed 4 times and resuspended in Laemmli buffer for western-blot analysis ...
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bioRxiv - Microbiology 2022Quote: ... 1:1000 dilution rabbit anti-human acetyl histone H3 (Active Motif, Cat. 39040), and 1:500 dilution mouse anti-human β-actin (Cell Signaling ...
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bioRxiv - Biophysics 2022Quote: ... and P1 virus was generated from cells transfected with FuGENE transfection reagent (Active Motif) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions with 3 × 105 cpm of labeled probe was performed for 20 min at 4°-8°C using Binding Buffer C-1 (Active Motif 37484) together with Stabilizing Solution D (Active Motif 37488) ...
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bioRxiv - Biophysics 2020Quote: ... and incubated with anti-Lamin A/C antibody 1:1000 (Active Motif, 39287) in block overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were blocked in 5 % milk/TBST for 1 h followed by primary antibody blotting at 4 °C overnight incubation – 1: 2000 rabbit anti-hmC (cat# 39069, Active Motif). The next day the blots were washed with TBST followed by incubation with secondary 1:2000 anti-rabbit-HRP for 1 h ...
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bioRxiv - Cell Biology 2021Quote: ... Equal intensity of different RNA probes were mixed with equal molar of different C/EBPα proteins individually in EMSA buffer B1 (Active Motif, Cat. No. 37480), stabilizing solution D (Active Motif ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Microbiology 2022Quote: ... Lamin A/C (Active Motif) and H3K9me2 (abcam) ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). Immunoprecipitated material and input chromatin were submitted to the Genomics Core Facility (GCF ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed using human positive and negative control qPCR primer sets from Active Motif (Additional file 1: Table S5). PCR primers were designed in the TSS region for selected genes (Additional file 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... After an overnight incubation at 4°C with the primary antibodies (anti-5mC, Eurogentec, ref BI-MECY-0100, 1/500; anti-5hmC, Active Motif, ref 39569, 1/500), and a 1-hour incubation at room temperature with secondary antibodies (Dylight 488 anti-mouse ...
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bioRxiv - Molecular Biology 2021Quote: Recombinant Flag-tagged human G9a (Active Motif, 31410), human calpain 1 (Novus ...
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bioRxiv - Molecular Biology 2019Quote: ... Chromatin samples were incubated for 5 min at 37°C followed by a second incubation for 10 min at 37°C with shaking at 1,000 rpm after the addition of 1:100 enzymatic working solution (Active Motif, ChIP-IT Enzymatic Kit ...
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bioRxiv - Genomics 2023Quote: ... Human Positive Control Primer Set MYT1 (Active Motif, #71007), Human Negative Control Primer Set 1 (Active Motif ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 3 (Active Motif, #71023). For analysis ...
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bioRxiv - Immunology 2019Quote: ... Blots were incubated over night at 4°C with the following primary antibodies diluted in 2 % skim milk powder/PBS-T: H3K27ac (1:1,000, rabbit, Active Motif, 39133) and H3 (1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant AGO2 protein (Active Motif) was purchased and serially diluted in Protein LoBind tubes (Eppendorf ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in PBS-T for 1 h at room temperature and incubated overnight at 4°C with primary antibodies against Lamin AC (Active Motif 39287), Lamin B1 (Abcam ab16048) ...
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bioRxiv - Genetics 2019Quote: ... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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bioRxiv - Molecular Biology 2023Quote: ... All incubations with primary antibodies were performed for either 1h at room temperature or overnight at 4°C with the following antibodies for H3K9me3 (Active Motif, 1:1000), DAXX (Santa Cruz ...
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bioRxiv - Microbiology 2021Quote: Nuclear proteins were isolated from challenged A549 cells using a nuclear protein extraction kit (Active Motif) according to manufacturer’s specifications and protein concentration was determined using a bicinchoninic acid (BCA ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant human PHD2(EGLN1) was purchased from Active Motif (USA, #81065) and HIF1α-ODD peptide (DLDLEALAPYIPADDDFQL ...
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bioRxiv - Neuroscience 2023Quote: ... myelin basic protein (Active Motif, 31314) was employed as a substrate for recombinant CDKL5 ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were diluted in blocking buffer and applied to cells overnight at 4°C to label key histone modifications associated with epigenetic regulation: rabbit H3K9me3 (1:500; Active Motif, 39765; Carlsbad, CA, USA); mouse H3K4me1 (1:100 ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.8μM of purified DNMT1 protein (Active Motif) was incubated with 30000 cpm of 32P-labelled aptamers and single or double-stranded ...
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bioRxiv - Cell Biology 2023Quote: The recombinant proteins p53 (#81091, Active Motif), PITPα (#ab101666 ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cell Biology 2020Quote: The following commercially available proteins were used in this study: recombinant SETD2 (a.a. 1418-1714) protein (Active Motif, 31358), rabbit skeletal muscle actin (Cytoskeleton ...
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bioRxiv - Immunology 2020Quote: ... Chromatin precipitation was performed using rabbit anti-human H3K4me3 IgG Ab (Active Motif) as described previously [48] ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4 °C or H3K27ac (Active motif; Catalog no: 39133). The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant Human DNMT1 with an N-terminal GST tag was purchased from Active Motif and Pierce Glutathione Magnetic Beads (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... KURAMOCHI and OVCAR4 Hi-C were conducted and processed by Active Motif using the Arima-Hi-C kit and library prep performed with technical duplicates (Arima Genomics) ...
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bioRxiv - Cell Biology 2023Quote: ... recombinant CDKL5 and myelin basic protein (Active Motif, 31314) as a substrate were incubated in a kinase buffer (Cell Signaling ...
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bioRxiv - Genetics 2019Quote: ... containing either 5 μg human liver or embryonic kidney nuclear extracts (Active Motif 36042; 36033), 2 μg of either anti c/EBPbeta (CEBPB ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and Lamin A/C (Active Motif #39288 Clone 3A6-4C11, 5 uL per IP). The IP was incubated overnight at 4C with nutation ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no ...
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bioRxiv - Neuroscience 2019Quote: ... In vitro cleavage assays were performed by adding 50nM human recombinant Ago2 (expressed in baculovirus, Active Motif) in a reaction mixture containing 25mM Hepes-KOH pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... or 100-750ng YY1 full-length recombinant protein (31332, Active Motif) in binding buffer containing 10 mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: Recombinant AGO (AGO1, AGO2, AGO3) protein was purchased from Active Motif (AGO1 Catalog No ...
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bioRxiv - Neuroscience 2023Quote: ... Mitochondrial proteins were extracted using Nuclear Extract Kit (Active Motif, Germany) according to manufacturer instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...