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Citations for Active Motif :
1 - 50 of 65 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and phospho S2 polymerase II (Active Motif, 61083, 1:200), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was eluted using DNA purification elution buffer (Active motif). The eluted DNA was used in qPCR using primers targeting putative cohesin-bound positions in the integration site (coordinates ...
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bioRxiv - Genetics 2019Quote: ... proteinase K and DNA purified using the ChIP DNA Purification kit (Active Motif #58002) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Global DNA methylation was studied using the global DNA methylation assay kit - LINE-1 (Active Motif) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Neuroscience 2021Quote: The Next Gen DNA Library Kit (Active Motif) includes molecular identifiers (MIDs) ...
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bioRxiv - Cell Biology 2023Quote: ... The Methylated DNA Immunoprecipitation (MeDIP) Kit (Active Motif) was used according to the instructions by the manufacturer with some modifications ...
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bioRxiv - Physiology 2022Quote: ... The activity of NF-κB in binding DNA was assessed in nuclear fractions by a DNA Binding TransAM NF-κBp65 Assay (Active Motif).
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bioRxiv - Cancer Biology 2020Quote: ... Immunoprecipitated DNA and input DNA were then purified with phenol/chloroform extraction and amplified using GenoMatrix Whole Genome Amplification kit (Active Motif). Quantitative PCR was performed using PowerUP SYBR Green master mix on an ABI StepOnePlus real-time PCR instrument (Applied Biosystems) ...
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bioRxiv - Genomics 2020Quote: ... The DNA samples were processed for library preparation for next-generation sequencing by using a Next Gen DNA Library Kit (Active Motif) following the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2021Quote: DNA from whole brain and neuronal nuclei ChIP samples was purified with the Chromatin IP DNA purification kit (Active Motif, #58002) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell fractionations were verified by western blot as described in 57 with antibodies against RNA polymerase II CTD phospho Ser2 (Active Motif, 61984) and GAPDH (GeneTex GT239 or ThermoFisher MA5-15738) ...
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bioRxiv - Molecular Biology 2021Quote: ... or the Chromatin IP DNA Purification Kit (Active Motif). The following primary antibodies were used for ChIP experiments ...
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bioRxiv - Genomics 2021Quote: ... DNA was sonicated to 200-300bp fragments (Epishear, Active Motif, 100% amplitude ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitated DNA fragments were collected by magnetic beads (Active Motif), purified ...
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bioRxiv - Genomics 2019Quote: ... Ligated DNA was sonicated to 200-300bp (Epishear, Active Motif, 100% amplitude ...
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bioRxiv - Neuroscience 2021Quote: ... the Next Gen DNA Library Kit (Active Motif, Cat# 53216) and Next Gen Indexing Kit (Active Motif ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified with the ChIP DNA Purification Kit (Active Motif). The qPCR assays were performed on a CFX96 qPCR system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA from in vitro methylated Jurkat cells were (Active Motif) served as positive control and genomic DNA from H1993 cells served as negative control ...
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bioRxiv - Cell Biology 2021Quote: ... A chromatin IP DNA Purification Kit (Cat No 58002 Active Motif) was used to purify the samples after CHIP before PCR was performed using Myh11 primers [5’-CCC TCC CTT TGC TAA ACA CA - 3’ and 5’ - CCA GAT CCT GGG TCC TTA CA – 3] ...
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bioRxiv - Cell Biology 2021Quote: ... A chromatin IP DNA Purification Kit (Cat No 58002 Active Motif) was used to purify the samples after CHIP before PCR was performed using Myh11 promoter primers [5’ -CCC TCC CTT TGC TAA ACA CA – 3′ and 5’ – CCA GAT CCT GGG TCC TTA CA – 3] as previously published [79] ...
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bioRxiv - Genetics 2022Quote: ... A Global DNA Methylation Assay Kit (Cat#55017, Active Motif, USA) was used for DNA methylation analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were generated by the Next Gen DNA Library Kit (Active Motif) with the Next Gen Indexing Kit (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... Fragmented DNA was immunoprecipitated with antibodies against mouse H4K20me3 (39672, Active Motif), or negative control mouse IgG (5415 ...
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bioRxiv - Molecular Biology 2023Quote: ... γH2AX (Active Motif # 39117; 1 mL per 1 mg DNA for CHIP); Senataxin (Novus biological # NB100-57542 ...
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bioRxiv - Developmental Biology 2021Quote: Libraries were prepared using the Next Gen DNA Library kit (Active Motif 53216) and Next Gen Indexing kit (Active Motif 53264 ...
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bioRxiv - Molecular Biology 2020Quote: ... The fragmented DNA was used in combination with the hydroxymethyl collector (Active Motif) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and mechanical DNA fragmentation using a ChIP-IT Express kit (Active Motif, #53008) and Bioruptor (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... The histone and DNA solution was incubated with 1μg H3K27ac (Active motif, 39133) or H3K4me3 (homemade77 ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA were immunoprecipitated using antibodies including anti-H3K27ac (Active Motif, #39133, Carlsbad, CA), anti-H3K27me3 (CST ...
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bioRxiv - Microbiology 2023Quote: ... DNA Methylation Profiling (RRBS Service) was performed by Active motif (Carlsbad, CA, USA); DNA digest ...
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bioRxiv - Microbiology 2023Quote: ... and total DNA tagmented and purified using the ATAC-seq kit (Active Motif) in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was then immunoprecipitated overnight using 2.5 μg of H3K27Ac (Active Motif 91193) or CTCF antibody (Active Motif 91285) ...
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bioRxiv - Cancer Biology 2020Quote: ... fragmented DNA was heat-denatured and immunoprecipitated with anti-5mC antibody (39649, Active Motif). An additional quantity of fragmented DNA equivalent to 10% of DNA being used in the immunoprecipitation reaction was also denatured and saved as input DNA ...
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bioRxiv - Cell Biology 2023Quote: Accessible genomic DNA was obtained for sequencing using an ATAC-seq kit (Active Motif) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Global DNA methylation LINE-1 were measured by Active Motif (55017, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-CTCF (Active Motif; Cat#: 61311) antibody overnight at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Quality of ChIP-enriched DNA was assessed using the ChIP-IT qPCR Analysis Kit (Active Motif).
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bioRxiv - Cell Biology 2022Quote: NF-κB DNA-binding activity was measured using the TransAM NF-κB activity assay (Active Motif). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... and genomic DNA regions of interest isolated using 4 μg of H3K27Ac antibody (Active Motif, cat# 39133). Complexes were washed ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA regions of interest were isolated using antibodies against RelA (Active Motif, catalog number 39369; RRID:AB_2793231) in articular chondrocytes ...
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: ... Genomic DNA regions of interest were immunoprecipitated using the following antibodies: EZH2 (Active Motif, Cat. No. 39901), H3K27me3 (ActiveMotif ...
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bioRxiv - Neuroscience 2022Quote: For DNA Chromatin Immunoprecipitation (ChIP) we used the ChIP-IT High Sensitivity® kit from Active motif. Lysates were obtained as described in the RIP protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-seq libraries were constructed using the Next Gen DNA Library Kit (Active Motif, 53216 and 53264). Library quality was assessed using Agilent 2100 Bioanalyzer and Agilent High Sensitivity DNA assay ...
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bioRxiv - Cell Biology 2023Quote: ... Genomic DNA regions of interest were isolated using 4ul of H3K36me3 (Active Motif cat# 61101, lot# 22264149) and 0.4ug of antibody against H2Av (Active Motif cat# 61686) ...
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bioRxiv - Cell Biology 2020Quote: Purified DNA fragments for ChIP-seq were prepared by using the ChIP-IT High Sensitivity Kit (Active Motif) and the related antibodies ...
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bioRxiv - Genomics 2019Quote: ... The libraries were generated with the Illumina Nextera DNA Library Prep kit and were sequenced by Active Motif and NIEHS Sequencing core by Illumina HiSeq 2000 and NextSeq 500 systems with 42bp paired-end reads on each sample ...
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bioRxiv - Genomics 2023Quote: ... Glucosylation reactions were performed in a 50µl solution containing sonicated DNA with UDP-Azide-Glucose (Active Motif 55020), T4 beta-glucosyltransferase and 10X EPi Buffer (Fisher Scientific FEREO0831 ...
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bioRxiv - Molecular Biology 2020Quote: ... Global % 5mC levels were assayed using a Global DNA Methylation LINE-1 Kit (55017; Active Motif, Carlsbad, CA, USA) according to the manufacturer’s instructions ...