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Citations for Active Motif :
1 - 50 of 439 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Magnetic beads (Active Motif) were then washed once with 1x PBS-Tween and combined with the antibody-lysate mixture ...
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bioRxiv - Genomics 2023Quote: ... Magnetic beads (Active Motif) were then washed once with 1× PBS-Tween and combined with the antibody-lysate mixture ...
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bioRxiv - Molecular Biology 2019Quote: ... using magnetic beads (Active Motif) diluted in commercial ChIP-buffer (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... 30μl of Protein G Magnetic Beads (Active Motif) were washed twice with 500μl CoIP buffer and incubated with Antibody-containing lysat for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitated DNA fragments were collected by magnetic beads (Active Motif), purified ...
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bioRxiv - Molecular Biology 2020Quote: Co-IP was done using magnetic beads Co-IP kit (Universal Magnetic CO-IP Kit, Active Motif, 54001-am) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... including Protein A magnetic beads as per manufacturer’s instructions (Active Motif, USA). The input and immunoprecipitated DNA were quantified by real-time PCR using a Stratagene MX3500P PCR System ...
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bioRxiv - Cell Biology 2023Quote: ... Immune complexes were collected using 20 μL of protein G magnetic beads (Active Motif). After elution from the beads ...
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bioRxiv - Developmental Biology 2023Quote: ... ChIP assay was done using SimpleChIP Plus Enzymatic Chromatin IP kit (magnetic beads) #9005 from CellSignalling and H3K27me3 antibody (Active Motif RRID:AB_2561020). ChIP samples were prepared with Kapa Hyper prep kit ...
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bioRxiv - Genomics 2022Quote: ... FLAG-MYC/MAX (Active Motif, Cat. nr. 81087), FLAG-SP1 (Active Motif ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-RING1B antibody was bound to IgG beads (#39663, Active Motif, 1:500) for 1h at RT before applying 500 μg of the total cell lysate ...
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bioRxiv - Molecular Biology 2021Quote: ... Immunoprecipitations for H2A.Z and HA-tag were performed similarly on benzonase-treated yeast cell extract using 36µl Protein-A sepharose beads coupled with 6µl of appropriate antibody (Anti-H2A.Z, Active Motif 39647; Anti-HA tag, Abcam ab9110). Control immunoprecipitations (mock ...
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bioRxiv - Physiology 2024Quote: ... The universal magnetic Co-IP kit (Active motif, 54002, Carlsbad, CA, USA) was used and the appropriate antibodies (specified in table 2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were immunoprecipitated using Universal Magnetic Co-IP Kit (Active Motif 54002), then analyzed by western blot ...
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bioRxiv - Cancer Biology 2024Quote: Co-IP was performed using a Universal Magnetic Co-IP kit (Active Motif). Briefly ...
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bioRxiv - Pathology 2023Quote: ... Cells were lysed to ChIP-IT Express Magnetic Chromatin Immunoprecipitation kit (Active Motif 53008). Optimal sonication conditions were determined previously in order to obtain DNA fragments of about 500 bp ...
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bioRxiv - Neuroscience 2020Quote: ... then placed on rotator and incubated overnight at 4°C with 25 uL of Protein G beads and 4 uL (1 mg/mL) anti-RNA polymerase II (102660; Active Motif). Immune complexes were sequentially washed according to kit instructions ...
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bioRxiv - Immunology 2022Quote: ... 1 × 105 BM pDCs sorted from Hdac3fl/fl control or Hdac3fl/fl CreERT2 littermates were bound to beads and were subjected for immunoprecipitation with primary anti-H3K27ac antibody (Active Motif) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... and Protein G beads (Active motif, #53014) were added for an additional 2 hours ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoprecipitations were performed using 30 μg of cell line chromatin and 6 μg of N-Myc antibody (Active Motif # 61185) or 4 μg of H3K27Ac antibody (Active Motif #39133) ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p ...
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bioRxiv - Genomics 2019Quote: Extraction of nuclear proteins and coimmunopreciptation were performed using the Universal Magnetic Co-IP Kit (Active Motif). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... At the same time protein G agarose beads (Active Motif) were washed and blocked with 5% BSA in ChIP buffer and left to rotate O/N in 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear fractions were extracted following the manufacturer’s instruction supplied with the Universal Magnetic co-IP kit (54002, Active Motif). 1000μg of proteins were immunoprecipitated using 5.0μg of antibody followed by protein G magnetic beads ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed five times with AM1 Wash Buffer (Active Motif) then DNA eluted as per ChIP-IT kit instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclear extraction of MDA-MB-231 cells was done according to manufactureŕs instructions (Universal Magnetic Co-IP Kit, Active Motif, 54002). Imaging flow cytometry analysis was conducted on an Amnis Imagestream Mk (>100,000 events ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed five times with Wash Buffer AM1 (Active Motif). ChIP DNA was eluted in 100 μl Elution Buffer AM4 (Active buffer) ...
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bioRxiv - Immunology 2021Quote: ... Chromatin was pre-cleared using 30 μl protein G agarose beads (Active Motif) pre-blocked with BSA per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 μL beads with 10 μg of the following antibodies – H3K4Me3 (Active Motif 28431), H3K27Me3 (Merck Millipore 07-449) ...
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bioRxiv - Immunology 2019Quote: ... Then beads were incubated with H3K27ac antibody (1 µg per IP, rabbit, Active Motif, 39133) or normal rabbit IgG (1 µg per IP ...
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bioRxiv - Plant Biology 2019Quote: ... Added antibody-conjugated protein G beads (Active Motif 61085 for Pol II Ser5p, Active Motif for Pol II Ser2p and Ab817 for total Pol II ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was removed and the beads washed with 665µL of ChIP Buffer (from Active Motif kit) by inverting the tube several times ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif, Anti H3 #9715 Cell Signaling), detection protocols have been summarized in Supplemental Table 4 ...
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bioRxiv - Cancer Biology 2023Quote: ... the bead/nuclei mixture was incubated overnight with a 1:100 dilution of primary antibody (H3K27ac (39133, Active Motif)) which was followed by secondary antibody (13-1047 ...
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bioRxiv - Cancer Biology 2024Quote: ... the immunoprecipitated samples (now bound to the beads) were washed four times with Co-IP wash buffer (Active Motif). The washed immunoprecipitated samples bound to beads were then resuspended in 20 μl of 2X Reducing Loading Buffer (130 mM Tris pH 6.8 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-WT1 and anti-EZH2 (1:1000 dilution, Active Motif) and anti-B-actin (1:10,000 dilution ...
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bioRxiv - Genetics 2019Quote: ... mouse anti-Polo [88] and rabbit anti-CID (Active Motif). These primary antibodies were combined with either a Cy3 ...
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bioRxiv - Cell Biology 2022Quote: ... with anti-His and anti-T120 phosphoantibody (Active motif, 39391).
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bioRxiv - Cell Biology 2023Quote: ... with anti-His and anti-T120 phosphoantibody (Active motif, 39391).
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bioRxiv - Cancer Biology 2020Quote: ... The following day the beads were washed once with ChIP Buffer I and twice with ChIP Buffer II (Active Motif) before elution with 50mM Glycine (pH 2.8 ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-H3 and rabbit anti-H3K4 trimethyl (Active Motif).
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bioRxiv - Genetics 2020Quote: ... containing primary antibody (rabbit anti-H3K9me3, Abcam ab8898; rabbit anti-H3K27me3, Cell Signaling Technology C36B11; rabbit anti-H3K4me3 Active Motif 39159 ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... anti-SET8 (Active Motif) or anti-α-tubulin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... and phosphatase inhibitors cocktails were added to no more than 15 µl of the beads slurry along with 20 µg MBP (Active Motif 31314). The kinase assay mixtures were incubated for 30-120 minutes at 30°C ...
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bioRxiv - Neuroscience 2021Quote: The rest of the ChIP experiment (beginning from “Protein G Agarose Bead Preparation”) was carried out using the reagents and protocols from the Low Cell ChIP-Seq Kit (Active Motif, 53084). In brief ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were Rb anti-EZH2 (CST) and Ms anti-H3K27me3 (Active Motif) were used ...
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bioRxiv - Microbiology 2021Quote: Anti-CTCF (61311) and anti-H4Ac (39925) antibodies was purchased from Active Motif, HPV18 L1 (ab69 ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore, Anti H3K56Ac #61061 Active Motif, Anti H3K9Ac #39038 Active Motif ...
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bioRxiv - Genomics 2020Quote: ... and incubated with the appropriate primary antibody for 1 h at room temperature (anti-RNAPII: 1:500, 61086, Active Motif; anti-H3K27ac: 1:500, 39133, Active Motif; anti-H3K27me3: 1:500, 39155, Active Motif; anti-RAD21 ...
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bioRxiv - Molecular Biology 2023Quote: ... and immunoprecipitation performed over night at 4 °C on a spinning wheel by adding 10 µl of the respective antibody (Anti H3K27Me3 #9733 Cell Signaling, Anti H3 #2650 Cell Signaling, Anti H3K56Ac #61061 Active Motif). Afterwards ...