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Citations for Vector Labs :
1 - 50 of 1588 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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bioRxiv - Molecular Biology 2022Quote: ... Capped RNAs of RNA-DNA hybrids were Biotinylated (Vector lab, cat. SP1100) and purified by MPG streptavidin beads (Takara ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2020Quote: ... RGCs were purified using two-step immune-panning on two Lectin coated negative panning plates (Bandeiraea simplicifolia, BSL-1; Vector Laboratories Ltd.) and a Thy1.2 antibody coated positive plate (Serotec MCA02R ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were blocked for 20 minutes with two drops of Vector Biotin Block (Avidin/Biotin Blocking kit. SP-2001. Vector Laboratories, Inc. Burlingame, CA). Samples were washed twice with PBS with Protein Block Serum-Free Ready-To-Use for 30 minutes at R.T ...
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bioRxiv - Neuroscience 2021Quote: ... and an immunoperoxidase system (Vectastain Elite ABC-Kit, Vector Laboratories Ltd., Peterborough, UK). Slices were rinsed ...
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bioRxiv - Cancer Biology 2019Quote: ... washed two times and mounted using VECTASHIELD antifade mounting media (Vector labs). Stack images were taken with an Olympus FV100 confocal ...
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bioRxiv - Systems Biology 2021Quote: ... A two-step technique (polymer, HRP-conjugated; Vector Laboratories, Burlingame, CA, USA) was used for visualization ...
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bioRxiv - Neuroscience 2022Quote: ... An avidin-biotin complex (ABC) system (Vectastain ABC Elite kit; Vector Laboratories, Burlingame, CA) was used to enhance detection of the immunocomplexes ...
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bioRxiv - Cancer Biology 2024Quote: ... The IHC signal was amplified with a biotin-avidin-peroxidase system (ABC Elite Kit Vector) and visualized using diaminobenzidine (DAB Kit, Vector Laboratories). For immunofluorescence ...
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bioRxiv - Neuroscience 2021Quote: ... brains were washed with PBS two times and mounted in Vectashield (Vector Lab), ready for confocal imaging.
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bioRxiv - Genetics 2023Quote: ... slides were divided into two squares using an ImmEdge hydrophobic pen (Vector Labs) and placed horizontally in a humid chamber followed by the addition of 10 μl of cell suspension per square ...
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bioRxiv - Microbiology 2023Quote: ... Slides probed with two antibodies were counterstained with ImmPACT Vector Red (Vector Labs). Slides were rinsed in water and counterstained with hematoxylin ...
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bioRxiv - Neuroscience 2022Quote: ... then the signal was amplified using the ABC system (VECTASTAIN Elite ABC kit, Vector Laboratories), as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Two 15-cm petri dish were incubated with BSL-1 (5μg/mL, Vector Labs) in D-PBS for at least 2 hours at room temperature (negative panning dishes) ...
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bioRxiv - Neuroscience 2022Quote: ... the cell suspension was sequentially panned on two BSL1 panning plates (Vector Laboratories, #L1100). To purify OPCs ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated with the ABC system (Vectastain Elite ABC HRP Kit, Vector Laboratories, #PK6100). Horseradish peroxidase conjugates and 3,3′-diaminobenzidine were used for visualization of immune complexes according to the manufacturer’s manual (SeraCare ...
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bioRxiv - Molecular Biology 2019Quote: ... Superfrost glass slides were divided into two squares using an ImmEdge hydrophobic pen (Vector Labs), then 110 μl of 1% paraformaldehyde (PFA ...
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bioRxiv - Systems Biology 2020Quote: ... washed two times and mounted using VECTASHIELD antifade mounting media (Vector labs Cat. H-1000). Confocal images were taken with an Olympus FV100 or a Nikon A1+ confocal ...
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bioRxiv - Microbiology 2020Quote: ... sections were incubated with an avidin/biotin-based peroxidase system (VECTASTAIN Elite ABC HRP Kit, Vector Laboratories) followed by substrate deposition with VECTOR NovaRED Peroxidase (HRP ...
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bioRxiv - Pathology 2020Quote: ... The sections were then detected by alkaline phosphatase detection system (Vectastain ABC-AP kit, Vector laboratories, Burlingame, CA) and a blue reaction product was produced by incubating sections with alkaline substrate (Vector blue AP substrate kit III ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoreactivity was revealed using the ABC-peroxidase system and the DAB substrate kit (Vector Laboratories; Burlingame, CA, USA), and the sections were counterstained with hematoxylin ...
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bioRxiv - Molecular Biology 2024Quote: ... Two μL of embryo mixture was mixed with 2 μL of VECTASHIELD (Vector Labs H-1000-10) on an 8-mm round cover glass #1.5 ...
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bioRxiv - Developmental Biology 2019Quote: ... followed by avidin-biotin-peroxidase complex (1:50; ABC kit, VECTASTAIN Elite ABC system, Vector Labs, Burlingame, CA, USA). Peroxidase was reacted in 0.03% 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2022Quote: ... A conventional immunohistochemical procedure was followed using a commercially available detection system (Vectorstain Elite Kit, Vector Laboratories, Burlingame, USA). Peroxidase activity was visualized using 3’-3’diaminobenzidine ...
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bioRxiv - Physiology 2021Quote: Two neuronal tracers were used: neurobiotin (1% in 0.25 M KCl, Neurobiotin Tracer®, Vector Laboratories, Burlingame, US) and rhodamine (1% in distilled water ...
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bioRxiv - Molecular Biology 2022Quote: ... S trimers were purified using a two- step purification protocol by Lentil Lectin from Galanthus Nivalis (Vector labs, catalog AL- 1243. ...
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bioRxiv - Cancer Biology 2022Quote: ... we added biotinylated secondary antibody and used ABC (Avidin/Biotin) system provided in Vectorstain ABC-AP kit (Vector Laboratories; AK5200) and Vector Red substrate kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... pH 8) slices were treated with a peroxidase-based enzymatic detection system (Vectastain Elite ABC kit (PK-6100, Vector Laboratories)) ...
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bioRxiv - Neuroscience 2021Quote: ... Two final washes in PBS were performed before the sections were mounted on glass slides using Vectashield (Vector Laboratories) and imaged on a Zeiss confocal microscope (Zeiss 710 NLO) ...
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bioRxiv - Molecular Biology 2024Quote: ... and washed two times for 5 min in MQ before mounting with Vectashield mounting media (Vector Laboratories, #H-1000). Lipid content was quantified using Qupath version 0.2.2 ...
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bioRxiv - Biochemistry 2019Quote: ... was carried out using a biotinylated secondary antibody together with the avidin-biotin system and the VECTOR peroxidase kit (PK-6100, Vector Laboratories) using diaminobenzidine tablets as a substrate.
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bioRxiv - Neuroscience 2021Quote: ... visualization of antigen-bound primary antibodies was carried out using a biotinylated secondary antibody together with the avidin-biotin system and the VECTOR peroxidase kit (PK-6100, Vector Laboratories) using both diaminobenzidine tablets (D4293 ...
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bioRxiv - Molecular Biology 2023Quote: ... free-floating sections were rinsed and bound antibodies were visualized by the Avidin-Biotin complex (ABC) amplification system (Vectastain ABC kit, Vector Laboratories) using 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit ...
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslip pieces before covering with VectaShield mounting medium (Vector Laboratories) and a coverslip.
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bioRxiv - Developmental Biology 2019Quote: ... Then the embryo was split in two parts: the head was used for genotyping and the hindgut was dissected and mounted in Vectashield (Vector Laboratories) for observation with confocal microscopy.
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bioRxiv - Neuroscience 2021Quote: ... To isolate OPC the single cell suspension was sequentially panned on two panning plates coated with anti-BSL 1 Griffonia simplificonia lectin (#L-1100, Vector Labs) and then incubated on a plate coated with anti-CD140a (#135902 ...
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bioRxiv - Pathology 2020Quote: ... 3% hydrogen peroxide in methanol was used to block the endogenous peroxidase for 20 min and washed with 1X PBS two times followed by blocking with horse serum (Vector Lab) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated for two hours in goat-anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) at a 1:200 concentration ...
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bioRxiv - Genetics 2022Quote: ... samples were washed two times in wash buffer for 30 minutes each at 37°C and mounted in VECTASHIELD with DAPI (Vector Labs).
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bioRxiv - Cell Biology 2019Quote: ... followed by avidin-biotin complex detection system (VECTASTAIN Elite ABC system, Vector Labs PK-6100) for 30 minutes and DAB-horseradish peroxidase substrate detection system ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBST before being incubated for two hours in 1:250 dilution of horse-anti-mouse (Vector Laboratories, UK), or ...
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bioRxiv - Neuroscience 2019Quote: ... All stainings were visualized using respective secondary antibodies conjugated with HRP or ABC-system (Vectastain Elite ABC HRP Kit, Vector Laboratories Cat# PK-6100) and 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were transferred and incubated onto two subsequent negative panning plates coated with unconjugated Griffonia (Bandeiraea) Simplicifolia Lectin 1 (BSL-1) (#L-1100; Vector Laboratories, Burlingame, CA) as previously described [37] ...
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bioRxiv - Neuroscience 2020Quote: ... Cone and rod photoreceptors were eliminated with two subsequent negative selection plates coated with peanut agglutinin (PNA) (#L-1070; Vector Laboratories, Burlingame, CA) and monoclonal rat anti-mouse CD73 (clone TY/23 ...
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bioRxiv - Neuroscience 2022Quote: ... at 1:200 for two hours at room temperature and mounted with Vectashield Hardset antifade mounting medium with DAPI (Vector Laboratories, H-1500). Imaging was performed on a Leica SP8 Confocal microscope using a 40x oil objective ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.
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bioRxiv - Neuroscience 2024Quote: ... Brains were mounted on slides between two number 1 glass coverslips (to prevent squishing of brains) and covered with Vectashield mounting medium (Vector Laboratories, Newark, CA) and a coverslip.