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Citations for Vector Labs :
1 - 50 of 1257 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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bioRxiv - Microbiology 2021Quote: ... Infected cells were visualized by labeling with anti-flavivirus E-glycoprotein antibody using a mouse VECTASTAIN ABC-horseradish peroxidase (HRP) kit (PK-4002; Vector Laboratories) and a DAB peroxidase substrate kit (SK-4100 ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Immunology 2022Quote: ... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and 2% normal goat serum (S-1000, Vector Laboratories), and incubated with primary antibodies and species-specific Alexa Fluor secondary antibodies (A32732A11029 ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were probed with 1.7 μg/ml biotin-conjugated ricin (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 10 mg/ml D-galactose and 10 mg/ml lactose to confirm specific ricin binding ...
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bioRxiv - Microbiology 2023Quote: ... SOSIP glycoproteins were eluted with 1M methyl-α-D-mannopyranoside (from Vector Laboratories or MilliporeSigma), filtered through 0.2 μm filter and concentrated using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Molecular Biology 2024Quote: ... glycoproteins were probed for 1 hour using biotinylated-TL (1:5000 dilution, Vector Laboratories Inc.) in 3% BSA blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were probed with 1.7 μg/mL biotin-conjugated RCA (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 30 mg/mL D-galactose and 30 mg/mL lactose to confirm specific RCA binding ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Microbiology 2023Quote: ... diluted in PBS containing 2% goat serum (Vector Laboratories, S-1000), 2% FBS (Atlanta ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... H&E (Vector Labs, Inc.) and Shorr (Millipore Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were blocked for 1-2 h in 2% NGS solution (S-1000, Vector Laboratories Inc; in PBS) on ice ...
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bioRxiv - Pathology 2020Quote: ... and PHA-E (Vector Laboratories, B-1125) was conjugated to Alexa-488 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... ovaries were washed again 2 times fast and 2 times during 20min under agitation before mounting on slides with Vectashield (H-1000, Vector Laboratories).
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bioRxiv - Cancer Biology 2021Quote: ... Fucosylated proteins were eluted twice with 50 uL AAL elution buffer B1 or 40 uL glycoprotein eluting solution for fucose-binding lectins (Vector Laboratories) at 4°C for 1 h per round ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... construct with a C-terminal eGFP tag and N-terminal FLAG tag was generated and subcloned into a shuttle vector provided by Vector Laboratories, Burlingame ...
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bioRxiv - Genetics 2024Quote: ... or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts ...
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bioRxiv - Neuroscience 2023Quote: H&E staining was performed on adjacent serial sections to the imaged sections following a standard H&E procedure (Vector Laboratories) to correlate ion images with specific histological brain regions ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then rinsed 2 x 5 min in PBST and 2 x 5 min in PBS before mounting with VectaShield mounting media (Vector Labs, H-1000).
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bioRxiv - Cancer Biology 2021Quote: ... Replicate slides were also stained H&E (Vector Labs) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 h at room temperature with biotinyl rabbit antibody (Vector Laboratories, 1:1000) in 0.5 % BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Two μL of embryo mixture was mixed with 2 μL of VECTASHIELD (Vector Labs H-1000-10) on an 8-mm round cover glass #1.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips coated with immuno-stained S2 cells were mounted on to glass slides using vector shield (Vector Laboratory) for confocal microscopy.
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bioRxiv - Microbiology 2022Quote: ... stained with hematoxylin and eosin (H&E) staining kit (Vector Laboratory), and mounted using permount (Fisher scientific) ...
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bioRxiv - Pathology 2021Quote: ... cecum sections were stained with hematoxylin & eosin (H&E; Vector Labs), and conditions were masked for a board-certified gastrointestinal pathologist to score edema ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation in ABC Media 1:800 (Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in VECTASTAIN Elite ABC Kit (1:800; Vector Laboratories; cat # PK-6100; RRID: AB_ 2336817) for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Sections later were processed using routine H&E staining (Vector Laboratories H3502) and cover slipped for imaging ...
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bioRxiv - Developmental Biology 2019Quote: ... blocked for 2 hours with a blocking buffer composed of 1xPBS, 0.1% Triton-X100, 2% BSA (A9418, SigmaAldrich) and 5% goat serum (S-1000, Vector Laboratories) at room temperature ...
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bioRxiv - Physiology 2020Quote: ... incubated in 4’6’-diamidino-2-phenylindole (DAPI) (1:1000) in TBS for 5 minutes then mounted in Vectashield (Vector Laboratories). Fluorescent images were captured using a Nikon A1R-s confocal laser scanning microscope at the BCM Integrated Microscopy Core and processed with the Nikon Perfect Focus System (Nikon Corporation ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Brain slices were stained with hematoxylin-eosin (H&E) (Vector Laboratories, Burlingame, California) to characterise pathological lesions and Ziehl-Neelsen staining (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... or goat anti-rabbit biotinylated (Vector Labs, BA-1000 1:1000) diluted in TBS-T+ 10% goat serum and followed by incubation with VECTASTAIN® Elite® ABC-HRP Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated secondary antibodies were from Vector Laboratories (BA-1000, 1:1000). Sections were developed following standard methods ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody labeling was amplified with biotinylated goat anti-rabbit IgG for 2 hr (1:500 in PBS, BA-1000, RRID: AB_2313606; Vector Laboratories; Burlingame, CA) followed by Vectastain ABC Solution for 1 hr (1:500 in PBS ...
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bioRxiv - Pathology 2021Quote: ... PRR4 and mucus were immunohistochemically stained by PRR4 antibody (1:1000) and biotinylated WGA (BK-1000, Vector Laboratories, 1:1000), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... biotinylated anti-rabbit immunoglobulin G (Vector Laboratories, BA-1000, 1:1000 dilution). All clinically approved drugs ...
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bioRxiv - Immunology 2023Quote: ... and anti-mouse βIII Tubulin (Table S2) was revealed using a mouse-on-mouse kit according to manufacturer’s instructions (Vector laboratories, Burlingame, CA).
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bioRxiv - Molecular Biology 2023Quote: H&E stains were performed according to the kit’s instructions (Vector Laboratories, H-3502) for pathological assessment and informed Region of Interest (ROI ...
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bioRxiv - Biochemistry 2024Quote: ... and RBD (lacking an LPETG tag) were biotinylated using the ChromaLINK biotinylation kit (Vector labs). Manufacturer’s instructions were used ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies were diluted 1:1000 (PI-1000 or PI-2000, Vector Laboratories). Bands were visualized using chemiluminescence (SuperSignal™ West Pico Chemiluminescent Substrate ...
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bioRxiv - Neuroscience 2019Quote: ... in 0.1M PBS and then reacted with avidin-biotin horseradish peroxidase complex (1:800 in 0.1M PBS; Vector Laboratories) for 1 h at RT ...