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Citations for Vector Labs :
1 - 50 of 424 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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bioRxiv - Biochemistry 2024Quote: ... and RBD (lacking an LPETG tag) were biotinylated using the ChromaLINK biotinylation kit (Vector labs). Manufacturer’s instructions were used ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Immunology 2022Quote: ... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were probed with 1.7 μg/ml biotin-conjugated ricin (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 10 mg/ml D-galactose and 10 mg/ml lactose to confirm specific ricin binding ...
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bioRxiv - Microbiology 2023Quote: ... SOSIP glycoproteins were eluted with 1M methyl-α-D-mannopyranoside (from Vector Laboratories or MilliporeSigma), filtered through 0.2 μm filter and concentrated using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Molecular Biology 2024Quote: ... glycoproteins were probed for 1 hour using biotinylated-TL (1:5000 dilution, Vector Laboratories Inc.) in 3% BSA blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were probed with 1.7 μg/mL biotin-conjugated RCA (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 30 mg/mL D-galactose and 30 mg/mL lactose to confirm specific RCA binding ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... Fucosylated proteins were eluted twice with 50 uL AAL elution buffer B1 or 40 uL glycoprotein eluting solution for fucose-binding lectins (Vector Laboratories) at 4°C for 1 h per round ...
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bioRxiv - Microbiology 2021Quote: ... Infected cells were visualized by labeling with anti-flavivirus E-glycoprotein antibody using a mouse VECTASTAIN ABC-horseradish peroxidase (HRP) kit (PK-4002; Vector Laboratories) and a DAB peroxidase substrate kit (SK-4100 ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... construct with a C-terminal eGFP tag and N-terminal FLAG tag was generated and subcloned into a shuttle vector provided by Vector Laboratories, Burlingame ...
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bioRxiv - Physiology 2023Quote: ... of paraffin-embedded tissues using specific commercially available primary antibodies (Supplementary Table S1) and AlexaFluor 488 and 568-conjugated F(ab’) secondary antibody (Molecular Probe) or VECTASTAIN ABC kit (Vector lab). Cell-specific CD68 positive and total cell numbers were counted in the area of 0.07244 mm2 ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... antigens were retrieved on the deparaffinized sections for detection with primary antibodies (Table S1) and further detected with a streptavidin-peroxidase reaction kit and DAB as a chromogen (ABC kit; Vector Labs, Burlingame, CA). To ensure the specificity of the primary antibody ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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bioRxiv - Immunology 2021Quote: ... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
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bioRxiv - Neuroscience 2021Quote: ... Neurobiotin (Vector Laboratories; 2%) was included in the internal recording solution to determine the recording location during post-hoc morphological reconstruction ...
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bioRxiv - Neuroscience 2019Quote: ... with 2% Neurobiotin (Vector Laboratories). The cortex was penetrated with a normal angle ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were blocked for 2 to 3 hrs with 2 % heat inactivated goat serum (Vector Laboratories) and 4 mg/ml BSA (AppliChem ...
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bioRxiv - Neuroscience 2023Quote: ... (1:2, Rat adsorbed, Vector laboratories) diluted in TBS ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Chromosomes were counterstained with DAPI (4’,6-diamidino-2-phenylindole; 2 μg/ml) in Vectashield (Vector Laboratories). Fluorescent signals were analyzed and photographed using a Zeiss Axioimager epifluorescence microscope and a CoolCube camera (MetaSystems) ...
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bioRxiv - Genetics 2019Quote: ... Chromosomes were counterstained with DAPI (4’,6-diamidino-2-phenylindole; 2 μg/ml) in Vectashield (Vector Laboratories). Fluorescence signals were analyzed with an Olympus BX-61 epifluorescence microscope and CoolCube CCD camera (MetaSystems) ...
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bioRxiv - Plant Biology 2020Quote: ... 15 μl of 2 μg/ml 4’,6 diamidino-2-phenylindol (DAPI) diluted in Vectashield (Vector Laboratories) was added to the slide and sealed with a glass cover slip ...
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bioRxiv - Plant Biology 2022Quote: ... Chromosomes were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, 2 µg/mL) in Vectashield antifade (Vector Laboratories). Fluorescence signals were analyzed and photographed using a Zeiss Axio Imager epifluorescence microscope with a CoolCube camera (MetaSystems ...
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bioRxiv - Microbiology 2019Quote: ... 6-diamidino-2-phenylindole (Vector Laboratories Inc.) and observed under Zeiss Apotome microscope at the specified magnification ...
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bioRxiv - Neuroscience 2021Quote: ... 6’-diamidino-2-phenylindole (DAPI; Vector Laboratories). Each in-situ hybridization included 1 slide for each group ...
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bioRxiv - Cancer Biology 2022Quote: ... and (2) Bloxall Blocking Solution (Vector Labs, SP-6000 ...
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bioRxiv - Biochemistry 2022Quote: ... 6-diamidino-2-phenylindole (DAPI)(Vector Laboratory) on poly-L-lysine coated microscope slides ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated-SNL (B-1305-2, Vector Laboratories); SNL-Cy5 (CL-1305-1 ...
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bioRxiv - Developmental Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Vector Lab).
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bioRxiv - Immunology 2023Quote: ... UEA1-FITC (Vector Laboratories, #FL-1061-2), Ly-51-AF647 (clone 6C3 ...
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bioRxiv - Neuroscience 2024Quote: ... biotinylated WFA (Vector laboratories #B-1355-2), rabbit-anti-HA (Cell Signaling #3724) ...
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bioRxiv - Neuroscience 2022Quote: ... the brains were blocked for 1-2 h in 2% NGS solution (S-1000, Vector Laboratories Inc; in PBS) on ice ...
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bioRxiv - Evolutionary Biology 2023Quote: ... After immunodetection, chromosomes were counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 2 µg/ml) in Vectashield (Vector Laboratories). The preparations were photographed using a Zeiss Axioimager Z2 epifluorescence microscope with a CoolCube camera (MetaSystems) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The preparations were stained with 4ʹ,6-diamidino-2-phenylindole (DAPI; 2 μg/ml) in Vectashield (Vector Laboratories, Peterborough, UK). Fluorescence signals were analysed using an Axioimager Z2 epifluorescence microscope (Zeiss ...
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bioRxiv - Microbiology 2023Quote: ... and stained with 2 µg mL-1 4′,6-diamidino-2-phenylindole (DAPI) in Vectashield (Vector Laboratories, Newark, CA, USA). The cells were enumerated using a Zeiss Axio Imager M2 epifluorescence microscope (Carl Zeiss AG ...
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bioRxiv - Neuroscience 2020Quote: ... and filled with 2-3% Neurobiotin (Vector Laboratories) dissolved in 0.5 M potassium chloride (9-19 MΩ) ...
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bioRxiv - Immunology 2023Quote: ... 1μl biotinylated UEA1 (Vector Laboratories, B-1065-2) was incubated with 25μl Biotin Binder Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 4′6-diamidino-2-phenylindole hydrochloride (Vector Laboratories). More than 20 randomly selected fields of view per sample were photographed using a WHN10×/22 eyepiece and a 60× objective (field of view is 1.1 mm ...
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bioRxiv - Cancer Biology 2023Quote: ... + 2% Animal Free Blocker (Vector Laboratories, SP-5030) in 0.1% PBS – Tween20 ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were counterstained with nuclear stain DAPI (2 μg/ml) for 2 min and covered with antifade medium Vectashield (Vector Laboratories). Slides were imaged with the Zeiss Axioplan 2 microscope and the ISIS software (version 5.7.4 ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with the nuclear stain DAPI (2 μg/ml) for 2 min and covered with antifade medium Vectashield (Vector Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... ovaries were washed again 2 times fast and 2 times during 20min under agitation before mounting on slides with Vectashield (H-1000, Vector Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... the slides were washed twice in 2xSCC and 15 μL of 2 μg/ml 4’,6 diamidino-2-phenylindol (DAPI) diluted in Vectashield (Vector Laboratories) were applied.
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bioRxiv - Developmental Biology 2023Quote: ... at RT for 2 hrs and then mounted in VECTASHIELD medium with 4′-6-Diamidino-2-phenylindole (DAPI) (Vector Lab Inc.).
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bioRxiv - Systems Biology 2020Quote: ... 1:200 LTL-FITC (Vector Laboratories, FL-1321-2), 1:500 PNA-Cy5 (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... UEA1-Rhodamine (Vector Laboratories #RL-1062-2, 2μg/ml), and Hoechst 33342 overnight at 4◦C ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) (Vector Labs, Peterborough, UK) to counterstain cell nuclei ...