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Citations for Vector Labs :
1 - 50 of 534 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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bioRxiv - Cancer Biology 2019Quote: ... counterstained with methyl green (Vector Laboratories). Brightfield images were taken using Zeiss Axioskop2 microscope with an AxioCam digital camera (Zeiss) ...
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bioRxiv - Immunology 2022Quote: ... To block non-specific protein binding either 2.5% goat or 2.5% horse serum (Vector Laboratories, Burlingame ...
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bioRxiv - Cancer Biology 2021Quote: ... or methyl green (H-3402, Vector Laboratories). For immunofluorescent staining ...
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bioRxiv - Neuroscience 2022Quote: ... counterstained with methyl green (H-3402; Vector Laboratories) and cover-slipped with Eukitt (FLUKA).
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bioRxiv - Developmental Biology 2020Quote: ... Tissue sections were counterstained with methyl green (Vector Laboratories), dehydrated in an ethanol series (95% ...
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bioRxiv - Developmental Biology 2023Quote: ... ABC binding (PK-6101, Vector laboratories) and followed by application of the DAB (K3468 ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were counterstained with methyl green (Vector Labs, H-3402).
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were visualized by incubation with Methyl green (#H-3402, Vector Laboratories) for 10 min at 60 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was visualized with 3,3′- diaminobenzidine (DAB; Vector Laboratories). Sections were then counterstained with hematoxylin (DAKO) ...
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bioRxiv - Neuroscience 2023Quote: ... Avidin–biotin–peroxidase complex binding (Vector Labs, 1:500, 1h) was performed next ...
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bioRxiv - Microbiology 2023Quote: ... SOSIP glycoproteins were eluted with 1M methyl-α-D-mannopyranoside (from Vector Laboratories or MilliporeSigma), filtered through 0.2 μm filter and concentrated using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Neuroscience 2020Quote: ... antibody binding was visualized using an ABC Vectastain Kit (Vector Laboratories), followed by detection with diaminobenzidine (Merck ...
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bioRxiv - Bioengineering 2020Quote: ... while nonspecific binding was blocked with 1% horse serum (Vector Labs) in PBS at room temperature for 45 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen-antibody binding was detected by Elite kit (PK-6100, Vector Laboratories) and DAB (DAKO ...
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bioRxiv - Biophysics 2023Quote: ... Unspecific binding was blocked with blocking buffer (5% horse serum (Vector Laboratories) supplemented with 0.05% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Pathology 2021Quote: ... Endogenous peroxidases and non-specific binding sites were blocked with Bloxall (Vector Labs) and horse serum (2.5% or 10% ...
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bioRxiv - Molecular Biology 2023Quote: ... The antigen-antibody binding was detected by Elite kit (PK-6100, Vector Laboratories) and DAB (DAKO ...
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bioRxiv - Cell Biology 2023Quote: ... nonspecific avidin binding was blocked by an avidin/biotin blocking kit (Vector laboratories) before incubating kidney sections with 1% BSA for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The antigen-antibody binding was detected by Elite kit (PK-6100, Vector Laboratories) and DAB (DAKO ...
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bioRxiv - Cancer Biology 2023Quote: ... and avidin binding sites was performed using Avidin/Biotin Blocking Kit (Vector Laboratories) per manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Nonspecific binding was blocked with animal-free blocker (SP-5035; Vector Laboratories, Burlingame, California) for 30 min at room temperature) ...
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bioRxiv - Pathology 2021Quote: ... and for non-specific binding sites (COL2: blocking solution from MoM Kit, Vector Labs, BMK-2202 ...
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bioRxiv - Developmental Biology 2020Quote: ... Antibody binding was detected with the ABC complex (Vectastain ABC kit, Vector Laboratories, Inc.). In the control sections ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was visualised using a Vector ImmPress kit (Vector Labs, Peterborough, UK) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed with 0.1%PBS-T for 15 min then counterstained with methyl green (H3042, Vector Laboratories, UK) for 10 min or haematoxylin QS (H3404-100 ...
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bioRxiv - Neuroscience 2021Quote: ... was used to visualise antibody binding along with 3,3-diaminobenzidine tetrahydrochloride (DAB) (Vector Laboratories, UK). All sections were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2019Quote: ... was used to visualise antibody binding along with 3,3-diaminobenzidine tetrahydrochloride (DAB) (Vector Laboratories, UK). All sections were counterstained with haematoxylin ...
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bioRxiv - Bioengineering 2021Quote: ... Visualization of the secondary biotinylated antibody binding was performed using NovaRED substrate kit (Vector Laboratories). Images of sections were obtained at 10x magnification with Elipse E200 upright microscope (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The antibody binding was visualized using a Vectastain ABC Kit (Vector Laboratories, cat# PK-6100) and diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2023Quote: ... and avidin binding sites were blocked using Avidin/ Biotin blocking kit (Vector laboratories, California, CA) followed by normal serum blocking and stained with anti-Green Florescence Protein (GFP ...
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bioRxiv - Pathology 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit secondary antibody (Vector Laboratories MP-7451) and visualized using ImmPACT DAB Peroxidase Substrate kit (Vector Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... Both E-Cadherin and CD31 staining have nuclear counter staining with VECTOR Methyl Green (Vector Laboratories, Inc., cat. H-3402-500). Microscopic imaging of histology samples were taken at 20X by using an Olympus microscope (BX63l ...
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bioRxiv - Cancer Biology 2023Quote: ... Specific antibody binding was visualized using the Vectastain Elite ABC Peroxidase kit (PK-6100; Vector Laboratories) according to manufacturer’s protocol with diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2019Quote: ... Endogenous biotin receptors and streptavidin binding sites were saturated using the Streptavidin/Biotin Blocking kit (Vector Laboratories). All antibodies were diluted in blocking solution ...
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bioRxiv - Biochemistry 2021Quote: ... Concentrated samples were incubated with GlcNAc-binding wheat germ agglutinin (WGA)-coated beads (Vector Laboratories, Burlingame, CA) at 4 °C overnight ...
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bioRxiv - Physiology 2021Quote: ... Antibody binding was then amplified by incubating with goat anti-rabbit secondary antibody (1:200, Vector Laboratories), followed by incubation with the ABC reagent (Vector Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... Positive antibody binding was detected using anti-rabbit IgG ImmPRESS-AP polymer reagent (Vector Laboratories, MP-5401) and visualized via a 10 min incubation in Vector Blue AP substrate (Vector Laboratories ...
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bioRxiv - Neuroscience 2022Quote: ... fixed brains were immunostained by first blocking non-specific binding with normal goat serum (Vector Laboratories, USA). Blocked samples were incubated overnight at 2–8ºC with rabbit anti-SLC2A1 polyclonal antibody (1:500 dilution ...
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Meniscal and ligament modifications in spontaneous and post-traumatic mouse models of osteoarthritisbioRxiv - Pathology 2019Quote: ... slides were blocked for endogenous Avidin/Biotin binding with an Aviding/Biotin Blocking Kit (Vector Labs, SP2001). Non-specific binding sites were blocked for 1 hour (Col2 ...
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bioRxiv - Biochemistry 2021Quote: ... Non-specific binding was blocked by using ‘Mouse in Mouse’ reagent (1:100 in PBS, Vector Laboratories). Brain slices were next incubated with the primary monoclonal antibody AT8 (1:500 ...
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bioRxiv - Pathology 2021Quote: ... for endogenous Avidin/Biotin binding with an Aviding/Biotin Blocking Kit (Vector Labs, SP2001; 15 minutes each), and for non-specific binding sites (COL2 ...
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bioRxiv - Genetics 2023Quote: ... Endogenous binding sites of biotin and avidin were blocked using an Avidin/Biotin blocking kit (Vector Laboratories). Sections were incubated in primary antibody cocktails overnight and secondary/tertiary antibodies for two hours at 4°C in a humid chamber with 1x TBST washes in between ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Biotinylated lectin binding was visualized by fluorescence and confocal laser microscopy of streptavidin-conjugated Daylight 488 (Vector Laboratories). Nuclei were stained with 4′,6-diamidino-2-phenylindole and were embedded with ProLong Gold mounting medium (Life Technologies) ...
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bioRxiv - Immunology 2020Quote: ... endogenous biotin and streptavidin binding sites were blocked according to manufacturer’s instructions (SP-2002, Vector Laboratories, Burlingame CA). For germinal center detection ...
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bioRxiv - Genetics 2020Quote: ... Non-specific antibody binding was blocked by preincubating embryos in 10% normal goat serum (S-1000, Vector Labs) and 0.5% Bovine Serum Albumin (BP1600 ...
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bioRxiv - Physiology 2021Quote: ... We prevented nonspecific binding of biotinylated secondary antibodies with an Avidin/Biotin blocking kit (Vector Laboratories, Burlingame, CA). We incubated goat anti-rabbi HRP-conjugated secondary antibodies in a humidified chamber for 45 minute at 1:200 dilution and quenched endogenous peroxidase activity by a 20 minute incubation of 3% H2O2 ...
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bioRxiv - Pathology 2023Quote: ... Non-specific binding of primary antibodies was blocked by goat serum (2.5% vol/vol; MP-7451, Vector laboratories) for 20 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: Antibody binding was detected with the Vectastain Elite ABC Kit Peroxidase (HRP) (Vector Laboratories, Burlingame, CA, United States) with a secondary antibody incubation of 30 min for all slides (Table 2) ...