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Citations for Vector Labs :
1 - 34 of 34 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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bioRxiv - Microbiology 2021Quote: ... Infected cells were visualized by labeling with anti-flavivirus E-glycoprotein antibody using a mouse VECTASTAIN ABC-horseradish peroxidase (HRP) kit (PK-4002; Vector Laboratories) and a DAB peroxidase substrate kit (SK-4100 ...
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bioRxiv - Immunology 2022Quote: ... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... Glycoproteins were probed with 1.7 μg/ml biotin-conjugated ricin (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 10 mg/ml D-galactose and 10 mg/ml lactose to confirm specific ricin binding ...
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bioRxiv - Microbiology 2023Quote: ... SOSIP glycoproteins were eluted with 1M methyl-α-D-mannopyranoside (from Vector Laboratories or MilliporeSigma), filtered through 0.2 μm filter and concentrated using Vivaspin 6 centrifugal concentrators (30kDa ...
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bioRxiv - Molecular Biology 2024Quote: ... glycoproteins were probed for 1 hour using biotinylated-TL (1:5000 dilution, Vector Laboratories Inc.) in 3% BSA blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Glycoproteins were probed with 1.7 μg/mL biotin-conjugated RCA (RCA-120, Vector Laboratories, UK) in blocking buffer before or after pre-incubation with 30 mg/mL D-galactose and 30 mg/mL lactose to confirm specific RCA binding ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... H&E (Vector Labs, Inc.) and Shorr (Millipore Sigma ...
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bioRxiv - Pathology 2020Quote: ... and PHA-E (Vector Laboratories, B-1125) was conjugated to Alexa-488 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Elution was done in 4 bed volumes with corresponding elution buffers (Glycoprotein Eluting Solution, Cat. No. ES-3100 Vector labs for AAL column and 0.1M Melibiose monohydrate for GSI Cat# AK-2401-2) ...
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bioRxiv - Cancer Biology 2021Quote: ... Fucosylated proteins were eluted twice with 50 uL AAL elution buffer B1 or 40 uL glycoprotein eluting solution for fucose-binding lectins (Vector Laboratories) at 4°C for 1 h per round ...
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... construct with a C-terminal eGFP tag and N-terminal FLAG tag was generated and subcloned into a shuttle vector provided by Vector Laboratories, Burlingame ...
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bioRxiv - Neuroscience 2023Quote: H&E staining was performed on adjacent serial sections to the imaged sections following a standard H&E procedure (Vector Laboratories) to correlate ion images with specific histological brain regions ...
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bioRxiv - Cancer Biology 2021Quote: ... Replicate slides were also stained H&E (Vector Labs) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... stained with hematoxylin and eosin (H&E) staining kit (Vector Laboratory), and mounted using permount (Fisher scientific) ...
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bioRxiv - Pathology 2021Quote: ... cecum sections were stained with hematoxylin & eosin (H&E; Vector Labs), and conditions were masked for a board-certified gastrointestinal pathologist to score edema ...
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bioRxiv - Microbiology 2023Quote: ... Sections later were processed using routine H&E staining (Vector Laboratories H3502) and cover slipped for imaging ...
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bioRxiv - Microbiology 2022Quote: ... Brain slices were stained with hematoxylin-eosin (H&E) (Vector Laboratories, Burlingame, California) to characterise pathological lesions and Ziehl-Neelsen staining (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... of paraffin-embedded tissues using specific commercially available primary antibodies (Supplementary Table S1) and AlexaFluor 488 and 568-conjugated F(ab’) secondary antibody (Molecular Probe) or VECTASTAIN ABC kit (Vector lab). Cell-specific CD68 positive and total cell numbers were counted in the area of 0.07244 mm2 ...
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bioRxiv - Molecular Biology 2023Quote: H&E stains were performed according to the kit’s instructions (Vector Laboratories, H-3502) for pathological assessment and informed Region of Interest (ROI ...
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bioRxiv - Biochemistry 2024Quote: ... and RBD (lacking an LPETG tag) were biotinylated using the ChromaLINK biotinylation kit (Vector labs). Manufacturer’s instructions were used ...
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bioRxiv - Physiology 2019Quote: ... Sections were also stained with hematoxylin and eosin (H&E, Vector Labs, Burlingame, CA, USA). Images of stained sections were taken on a microscope (BX51 ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... antigens were retrieved on the deparaffinized sections for detection with primary antibodies (Table S1) and further detected with a streptavidin-peroxidase reaction kit and DAB as a chromogen (ABC kit; Vector Labs, Burlingame, CA). To ensure the specificity of the primary antibody ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... or the e-beam coated gold slides were silanized by VECTABOND Reagent (Vector Labs, SP-1800-7) per the protocol from the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... Cytiva) for His-tagged monomers and gravity flow columns filled with Agarose bound Galnthus Nivalis Lectin beads (Vector Labs) for nanoparticles ...
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bioRxiv - Genomics 2021Quote: ... mouse Anti-E-Cadherin (BD Transduction; 610182; 1:200, lotus tetragonolobus lectin (LTL) (Vector Labs; B-1325; 1:100), Anti-VCAM1 (abcam ...
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bioRxiv - Cell Biology 2019Quote: ... Both E-Cadherin and CD31 staining have nuclear counter staining with VECTOR Methyl Green (Vector Laboratories, Inc., cat. H-3402-500). Microscopic imaging of histology samples were taken at 20X by using an Olympus microscope (BX63l ...
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bioRxiv - Microbiology 2021Quote: ... 7-10 μm thick cryostat sections were immobilized on Superfrost plus microscopy slides (Thermo scientifics) and prepared either for haematoxylin and eosin (H&E) stain or immunostaining using the Mouse on Mouse kit (Vector laboratories) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 5 μm thick sections were cut and stained with hematoxylin and eosin (H&E) or subjected to immunostaining using the Vectastain ABC kit (Vector Laboratories) including antigen retrieval in boiling citrate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... sections were mounted onto a Superfrost slide and stained with an H & E stain kit (#3502 Vector Laboratories, Newark, CA, USA) by following procedures suggested by the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... permeabilized using 0.1% Triton-X and processed for CDH1 (E-Cadherin) staining using the VIP peroxidase substrate kit (Vector labs SK4600) and anti-mouse E-Cadherin primary antibody (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... stained with anti-E antibody and visualized with a VECTASTAIN Elite ABC anti-mouse IgG kit with a VIP substrate (Vector Laboratories, Burlingame, CA USA). The entire wells of 48-well plates were photographed by Nikon DSLR camera D810 ...