Labshake search
Citations for Mabtech :
1 - 50 of 73 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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bioRxiv - Immunology 2020Quote: The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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bioRxiv - Immunology 2020Quote: ... and a commercial SARS-CoV-2 peptide pool (SARS-CoV-2 S N M O defined peptide pool (3622-1; Mabtech, Sweden) were included as assay controls ...
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bioRxiv - Immunology 2021Quote: S1 and S N M O specific IFN-γ and IL-2-secreting T cells were detected using the Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kit (Mabtech AB)6 ...
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bioRxiv - Immunology 2021Quote: ... The number of B cells secreting SARS-CoV-2 RBD-specific IgG and total IgG were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS kit (Mabtech AB)6.
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Immunology 2020Quote: ... a commercial COVID peptide pool (SARS-CoV-2 S N M O defined peptide pool [3622-1] – Mabtech), and CEF were included as assay controls at a concentration of 1µg/ml ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 S antigen-specific T lymphocyte response in mice was assessed by IFN-γ ELISpotPLUS kits (Mabtech) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB) 20 ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2021Quote: ... and visualization by Streptavidin horseradish peroxidase (HRP) (MABTECH Cat# 3310-9-1000).
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bioRxiv - Immunology 2021Quote: ... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
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bioRxiv - Immunology 2021Quote: ... and envelope glycoprotein B was purchased from Mabtech.
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 (Mabtech, 3441-4HPW-2), IL-4 (Dakewe Biotech ...
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bioRxiv - Immunology 2021Quote: Mouse IL-2 (MABTECH, 3441-4APW-2), IL-4 (MABTECH ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2021Quote: ... IL-4 (MABTECH, 3311-4APW-2), and IL-10 (MABTECH ...
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bioRxiv - Immunology 2023Quote: ... and IL-2 (Mabtech #MT2A91/2C95) and blocked with R-10 medium ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2021Quote: ... and IL-10 (MABTECH, 3432-4APW-2) ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH ...
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bioRxiv - Immunology 2021Quote: ... IFNγ-BAM and IL-2-biotin (MABTECH). This was followed by incubation with fluorophore labelled secondary antibodies ...
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bioRxiv - Microbiology 2024Quote: ... and IL-5 (Mabtech, 3391-4HPW-2) precoated ELISpot kits according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IL-2 or IL-4 ELISpotPLUS kits (MabTech) were used to determine SARS-CoV-2 S antigen-specific T lymphocyte response ...
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bioRxiv - Immunology 2021Quote: Monkey IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI10+ media ...
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bioRxiv - Immunology 2022Quote: Cellular immune responses in mice were performed using mouse IFN-γ/IL-4 ELISPOT PLUS plates (3321-4AST-2/3311-4APW-2, MABTECH, Sweden). 96-well ELISPOT plates were pre-treated as the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and with biotinylated mAb for IL-2 (Mabtech #MT8G10), then incubated with streptavidin-HRP and developed with Vector Blue for ALP and Vector NovaRed for HRP (Vector Laboratories) ...
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bioRxiv - Immunology 2020Quote: ... at 37 °C and 5% CO2 with overlapping peptide pools representative of either S1 or S2 SARS-CoV-2 proteins (JPT, #PM-WCPV-S) at 1 μg/ml each or DMSO in 96-well ELISpot IFN-γ plates (Mabtech #3321-4APW-2) in triplicates ...
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bioRxiv - Cancer Biology 2021Quote: ... Mouse IFN-γ ELISpot PLUS kit (MABTECH 3321-4APT-2) manufacturer protocol was followed for development of the spots ...
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bioRxiv - Immunology 2023Quote: ... and IFN-γ/IL-2/TNF-α (X-41A42B45W; Mabtech) FluoroSPOT were respectively performed ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse IL-17A ELISpotPLUS kit ALP (MABTECH, Cat# 3521-4APW-2) was performed following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Each peptide pool (2 µg/ml) and αCD28 (0.1 µg/ml, Mabtech) were added to the cells and plates were incubated for 48h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... were coated with monoclonal antibodies to bovine IFNγ and IL-2 (MABTECH). Duplicate wells were seeded with 1×105 cells per well of cryopreserved bovine PBMCs (rested overnight before use ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Serum samples were diluted 1:2 in ready-to-use ELISA diluent (Mabtech) prior to performing the IL-6 ELISA assay.
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Immunology 2020Quote: ... were coated with human IFN-γ antibody (Clone 1-D1K, 2 μg/ml, Mabtech, Sweden) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2024Quote: ... Frequencies of IFN-γ or IL-2-secreting T cells were assessed using commercial kits (Mabtech) according to the manuals ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...