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Citations for Mabtech :
1 - 50 of 256 citations for Creatinine Serum Low Sample Volume Kit 384 well Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... aliquots of PBMC (105/well) were seeded in duplicate wells of 96-well plates (MABTECH) pre-coated with the primary IFN-γ antibody and stimulated with mitogens PHA ...
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bioRxiv - Immunology 2021Quote: ... Immobilon-FL PVDF 96-well plates (Mabtech) were coated overnight at 4°C with mouse anti-human IFN-γ (clone 1-D1K) ...
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs (2-2.5 × 105/well) were stimulated for 16-18 hours in a 96-well pre-coated plate (ELISpotPLUS kit, MABTECH Inc., Mariemont, OH) at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Microbiology 2021Quote: ... Splenocytes were counted and 100,000 cells/well added to the plates of the Mouse IFN-g ELISpotPLUS (HRP) kit (MABTECH). Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... The 96-well ELISpot plates (MabTech, Cat# 3654-TP-10) were pre-wetted with 35% ethanol and washed five times with sterile water plus 1 time with PBS ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were plated in each well of the pre-coated Immobilon-FL PVDF 96 well plates (Mabtech), stimulated with the respective antigen at the respective concentration of 5 μg/ml and incubated at 37°C in a humidified CO2 incubator for 20-24 hrs ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2021Quote: IFNγ ELISpot assays were performed using pre-coated 96-well plates (Mabtech, Monkey IFNγ ELISPOT PLUS ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Serum samples were diluted 1:2 in ready-to-use ELISA diluent (Mabtech) prior to performing the IL-6 ELISA assay.
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bioRxiv - Immunology 2020Quote: ... Serum samples were diluted 1:250 in ELISA diluent (Mabtech, Sweden, Cat: 3652-D2). Diluted serum samples were added 100μl/well in duplicates and incubated for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Granzyme-B (Gz-B) FLUORISpot kit plates (Mabtech, Inc.) and developed after 48h according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... Ninety six-well mouse IFN-γ ELISPOT kit (Mabtech, USA) plates pre-coated with anti-mouse IFN-γ capture antibody were rinsed 5 times with PBS to hydrate the membrane and then blocked with supplemented CTL media for at least 30 minutes ...
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bioRxiv - Immunology 2020Quote: IL-6 levels in serum from patients and healthy controls were measured in freshly thawed serum using human IL-6 ELISA development kit (Mabtech), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 96-well nitrocellulose-bottom plates pre-coated with anti-mouse IFNγ monoclonal antibody were purchased from Mabtech. The plates were blocked with RPMI-10% FBS for at least 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2019Quote: ... and 1-2×105 cells were plated per well of a 96-well Millipore MAIPSWU plate coated with anti-IFNγ antibody according to the manufacturer’s instructions (3420-2HW-Plus, Mabtech Inc.). Peptide pools were added to the cells at a final concentration of 1μg/mL/peptide prior to incubation at 37°C overnight ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2022Quote: ... This cell and peptide mixture was loaded onto a 96-well PVDF plate coated with anti-IFN-γ (3420-2HW-Plus, Mabtech, Nacka, Sweden) and cultured overnight ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2024Quote: ... Plates (Mabtech) were coated overnight at 4°C with an antibody mixture of mouse anti-human IFNγ (clone 1-D1K) ...
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bioRxiv - Immunology 2020Quote: ... at 37 °C and 5% CO2 with overlapping peptide pools representative of either S1 or S2 SARS-CoV-2 proteins (JPT, #PM-WCPV-S) at 1 μg/ml each or DMSO in 96-well ELISpot IFN-γ plates (Mabtech #3321-4APW-2) in triplicates ...
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bioRxiv - Immunology 2021Quote: ELISpot plates (Mabtech) were pre-coated with recombinant SARS-CoV-2 trimeric spike (1μg/ml) ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... IFN-γ+ ELISPOTs assays were performed using 105 cells/well with the Hamster IFN-γ ELISPOTBASIC kit (Mabtech AB) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Plates (Mabtech, Nacka Strand, Sweden) were coated overnight at 4°C with an antibody mixture of mouse anti-human IFNγ clone (clone 1-D1K) ...
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bioRxiv - Immunology 2021Quote: Antigen-specific circulating PBMCs were evaluated using pre-coated FluoroSpot plates and kits purchased from Mabtech (Mabtech AB, Nacka Strand, Sweden) and used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were cultured on anti-IgG ELISpot plates and developed using the standardized ELISpot kit (Mabtech Inc., Cincinnati, OH, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech). Antigen-specific T cell responses were quantified by subtracting the number of spots in unstimulated cells from the peptide stimulated cells ...
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bioRxiv - Immunology 2022Quote: ... Monkey IFN-γ ELISpot plates (Mabtech) were used ...
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bioRxiv - Immunology 2023Quote: ... ELISpot plates (Mabtech, #3654-TP-10) were pre-wet with 15 μl/well of freshly prepared 35% ethanol for < 1 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and ELISPOT plates were developed following the manufacturer’s protocol for the ELISpot Plus: Mouse IFN-γ (ALP) kit (Mabtech Cat. No. 3321-4APW). After the plates were developed ...
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Immunology 2021Quote: ... Fluorospot plate (Mabtech, Cat no. 3654-FL) was coated with 100μL RBD (50μg/mL ...
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bioRxiv - Immunology 2022Quote: ... 50 µL/well of Fluorescent enhancer II (Mabtech) were added followed by incubation for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2021Quote: ... Fluorescent spots indicating a single mASC and relative volume of antibody produced were detected with an IRIS FluoroSpot Reader and counted with Apex software (Mabtech). Positivity thresholds were set for both S1 and N mASCs based on prepandemic control samples’ average S1 or N plus three standard deviations.
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bioRxiv - Immunology 2021Quote: ... ELISPOT assay plates (MabTech Inc., Cincinnati, OH, USA) specific for the detection of primate IFNγ were used according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: Pre-coated Ferret IFNγ-ELISpot (ALP) plates (Mabtech) were used according to the manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: Precoated monoclonal antibody IL-4 ELISPOT plates (Mabtech) were washed and blocked ...
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bioRxiv - Immunology 2020Quote: ... plates were treated with a Fluorescence enhancer (Mabtech) to optimize detection and then air-dried ...
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bioRxiv - Immunology 2021Quote: ... plates were incubated with a fluorescence enhancer (MABTECH). Cytokine producing cells were counted using an automated fluorescence plate reader (AID-iSpot reader ...
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bioRxiv - Immunology 2021Quote: ... plates were treated with a Fluorescence enhancer (Mabtech) to optimize detection and then air-dried ...
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bioRxiv - Immunology 2021Quote: Monkey IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI10+ media ...
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bioRxiv - Immunology 2021Quote: ... Fluorospot plates were developed according to manufacturer’s instructions (Mabtech). Briefly ...