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Citations for PerkinElmer :
1 - 50 of 95 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
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bioRxiv - Bioengineering 2023Quote: ... of approximately 5.7 ± 3.0 pmol/mg protein measured by the manufacturer using [3H]-DAMGO (ES-542-C OP3 ValiScreen, PerkinElmer, Waltham, MA) were obtained and cultured for a series of binding assays ...
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bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...
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bioRxiv - Immunology 2020Quote: ... Agencourt AMPure XP beads and PCR amplified using KAPA hot start High-Fidelity 2X PCR Master Mix and NextFlex index primers (Bioo Scientific, PerkinElmer) for 12 cycle by following thermocycler cycles ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Semi-quantitative RT-PCR was carried out in the presence of [α-32P] dCTP (Perkin Elmer) with primer sequences provided in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2020Quote: We incubated 5′-labeled 32P-miR-1236 (0.5 nM; PerkinElmer, Boston, MA, USA) with increasing concentrations of in vitro-transcribed WT or 402C>G FOXL2 mRNAs (0 ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Bioengineering 2020Quote: ... Azide functionalization was confirmed with Fourier-transform infrared spectroscopy (FTIR, Supplemental Figure 1, F Perkin Elmer Frontier MIR/NIR spectrometer) and quantified with electrospray ionization-mass spectrometry (ESI-MS ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
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bioRxiv - Bioengineering 2020Quote: ... Each set of images was quantified via Living Imaging Software (Perkin Elmer) and normalized to a non-fluorescent control organ set.
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
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bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
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bioRxiv - Cell Biology 2023Quote: ... Each primer was 5ʹ-labeled with ψ-32P-ATP (PerkinElmer) by T4 PNK (polynucleotide kinase ...
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bioRxiv - Microbiology 2020Quote: ... 100 µM of 5’-phosphorylated 4-nt primer ACCA (Perkin Elmer) and 5% DMSO containing ERDRP-0519 at the specified concentration ...
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bioRxiv - Biochemistry 2023Quote: ... the microplate was immediately set on fluorescence intensity reader (Perkin Elmer Multimode Plate Reader, EnVision 2105), monitored for 30min ...
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bioRxiv - Microbiology 2022Quote: ... coated (10%, 1.5h, RT) Cell Carrier-96 well plate (Perkin Elmer). After centrifugation (5 min ...
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bioRxiv - Microbiology 2023Quote: ... coated (10%, 1.5h, RT) Cell Carrier-96 well plate (Perkin Elmer). After centrifugation (5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and qRNA-Seq Barcoded Primer (sequence: AACGCCAT; all from the PerkinElmer kit). The following settings were applied for the PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... in PBS for 1h at RT and with TNB blocking buffer (PerkinElmer) for 30 min for EF5 staining ...
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bioRxiv - Molecular Biology 2021Quote: ... The primer extension assay was performed in a 384-well plate (PerkinElmer, 6008269). The reaction buffer contained 20mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 5 minutes at room temperature (RT) in accordance with manufacturer’s instructions (PerkinElmer). After 3 times wash by TNT ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in TNB (Blocking Reagent [Perkin Elmer; 0.05% in TN]; 30 min, RT), incubated with anti-DIG-POD antibody (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... Primers were labeled with 32P gamma-ATP (6000 Ci/ mmol, 25 mM, Perkin Elmer) with PNK (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... the downstream primer Damino was 5‘-phosphorylated and radiolabeled using [γ-32P]ATP (PerkinElmer) and T4 polynucleotide kinase (NEB ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... washed and finally incubated for 8 min at RT with Tyramide-Cy5 (Perkin Elmer) dil ...
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bioRxiv - Cancer Biology 2024Quote: ... by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer). The same protocol was adapted to measure the effect of paracrine signaling on the proliferation rates of parental PNT1A (1.0 × 103 cells/well) ...
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bioRxiv - Neuroscience 2021Quote: ... circulating levels of minocycline were measured from plasma collected in a sub-set of subjects at the end of the experiment using a commercially available tetracycline enzyme immunoassay (Perkin Elmer, Akron ...
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bioRxiv - Cancer Biology 2019Quote: ... Green fluorescent nucleic acid stain signal was measured with FITC filter set on the Envision 2103 multilabel microplate reader (Perkin Elmer). The background fluorescence of medium-only with CyQuant reagent was subtracted for data normalization.
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bioRxiv - Cell Biology 2021Quote: The data was collected in a set of 2 experiments for 9 compounds as follows: 384 well plates (PE cell carrier ultra, PerkinElmer, 6057308) containing 12,000 cells per well in RPMI1640 medium (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The primer was labeled on its 5’end with (γ 32P)-ATP (Perkin Elmer, 3000 Ci.mmol−1) and T4 polynucleotide kinase (Thermofisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... The donor and acceptor fluorescence were recorded using a set of single-molecule avalanche photodiodes (SPADs) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 513/17 and 615/45 band pass emission filter (Omega ...
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bioRxiv - Neuroscience 2021Quote: ... for 1h at RT with following blocking with TSA blocking reagent (Perkin Elmer, Waltham, MA, USA, FP1020) as 0.5% solution in PBS pH 7.4 for another 1h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were incubated at RT for 30 min and measured with EnVision multi-plate reader (PerkinElmer).
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bioRxiv - Biochemistry 2021Quote: ... an optimized set of 4 small interfering RNAs (siRNAs; SMARTPool) targeted against human Furin were purchased from Horizon Discoveries (Perkin Elmer, Lafayette, LA, USA) and transfections of HeLa cells were carried out using INTERFERin (PolyPlus ...
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bioRxiv - Immunology 2019Quote: ... and scanned at RT using 20X objective on the Vectra® 3 automated quantitative pathology imaging system (PerkinElmer) (Fig ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 30 min at RT with streptavidin-HRP (Perkin Elmer) dil ...
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bioRxiv - Microbiology 2020Quote: ... for 5 min at RT and measured at 450 nm by the microplate reader Victor Nivo (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: Culture supernatants were assayed for RT activity by surveying for 3H-thymidine incorporation (3H-TTP; Perkin Elmer #NET221A001MC). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Generated AFC signal is detected after 15 min at RT using Ex/Em = 360/460 nM (Envision, PerkinElmer). Final assay concentrations ...
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bioRxiv - Microbiology 2019Quote: ... primer (5’-AGUUGUUGAUC) was 32P-labeled at the 5′ position upon incubation with [γ-32P] ATP (PerkinElmer, Waltham, MA) and T4 polynucleotide kinase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Santa Rosa CA) and 1μL each of ITS1F and ITS4 primers (10μM, Dikarya) and run on a GeneAmp 2400 (Perkin Elmer) thermal cycler with the following conditions ...
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bioRxiv - Bioengineering 2023Quote: ... with primers from IDT (Leuven, Belgium) and analyzed on a LabChip® GX Touch Nucleic Acid Analyzer (Perkin Elmer). Plasmids with correct fragment size were amplified by PCR using Q5® High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Immunology 2022Quote: ... PCR products are visualized on Caliper LabChip (PerkinElmer).
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bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
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bioRxiv - Biophysics 2019Quote: ... The bead slurry was incubated at RT for 30 minutes before reading on an EnVision plate reader (Perkin Elmer).
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bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
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bioRxiv - Genomics 2022Quote: ... The slides were blocked in TNB buffer at RT for 30 minutes and incubated with antifluorescein-HRP Conjugate (PerkinElmer) in TNB (diluted 1:1000 ...
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bioRxiv - Microbiology 2020Quote: Plasmid DNA was sequenced on both strands by primer walking using the ABI PRISMDye Terminator Cycle Sequencing Ready Reaction kit (PerkinElmer) and an ABI PRISM 377 sequencer (Perkin-Elmer ...