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Citations for PerkinElmer :
1 - 50 of 1082 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 cells were seeded into white 96-well plates (PerkinElmer) at 20,000 cells/well and cultured overnight at 37 °C with 5 % CO2 in 100 µl DMEM supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Immunology 2021Quote: ... Vero E6 cells were seeded at 20,000 cells/well in culture medium into white 96-well plates (PerkinElmer, 6005688) and cultured overnight at 37°C 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... 50 μL of virus-antibody mixture was inoculated onto each well containing a Vero-E6 cell monolayer culture in 96-well plates (Cell Carrier Ultra plates, Perkin Elmer) that had been seeded the day before at 15,000 cells per well ...
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Immunology 2023Quote: ... 2x104 Vero E6-TMPRSS2 cells per well were seeded in 96-well luminometer-compatible tissue culture plates (Perkin Elmer) and incubated overnight ...
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bioRxiv - Immunology 2023Quote: Vero E6 cells were grown in DMEM supplemented with 10% FBS and seeded into white-walled 96 well plates (PerkinElmer, 6005688) at a density of 20,000 cells per well ...
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysates were combined with 8mL Ultima Gold scintillation cocktail (PerkinElmer 6013329) and CPM was measured using Beckman LS6500 Scintillation counter ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were transferred to a 384 OptiPlate (Perkin Elmer, Waltham, MA, USA) and mixed 1:1 with antibody mixture from the IP-ONE-Gq kit ...
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bioRxiv - Biochemistry 2019Quote: ... cell lysate (5 μl/well) was transferred to a 384-well ProxiPlate (PerkinElmer) and added to the donor and acceptor reaction mix (2.5 μl/ well ...
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bioRxiv - Immunology 2022Quote: ... 110 μl of the cell lysate was transferred to a black/white plate (PerkinElmer). Luminescence was measured using a PerkinElmer Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Four μL of cell lysate was transferred to a 384 well ProxiPlate (Perkin Elmer). Immediately prior to use ...
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bioRxiv - Microbiology 2020Quote: ... All virus supernatant was quantified via p24 ELISA (Perkin Elmer).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cell lysate (5 μL/well) was transferred to a 384-well ProxiPlate (PerkinElmer, Melbourne, Australia) and added to the donor and acceptor reaction mix (2.5 μL/ well ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were incubated with 1 mM GTP/[α-32P]GTP (0.1 μCi/μl) (PerkinElmer) and incubated at 30°C for various time intervals for comparison pYfiN and pYfiN(Ips) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were imaged and percent of virus infected cells calculated using the Operetta High Content Imaging System and Harmony® High Content Imaging and Analysis Software (PerkinElmer).
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bioRxiv - Genomics 2022Quote: ... Total RNA was extracted from the female Epstein-Barr virus transformed B-lymphocyte cell line GM24143 using the Chemagic™ RNA TissuelO Kit H96 (PerkinElmer) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Serum-virus mixtures were then transferred to 96-well plates (PerkinElmer), and 1.0×104 MDCK-SIAT1-PB1 cells51,92 were added into each well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of the cell lysates were transferred into a white 384 well plate (ProxiPlate, PerkinElmer). 5 μL of the Europium-cAMP tracer (1 to 50 dilution of Eu-tracer stock in cAMP detection buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... pseudotyped virus treated hESC-derived cardiomyocytes in CellCarrier-96 Ultra Plates (PerkinElmer) were washed 3x with 100 μL PBS before complete submersion in 2-4% formaldehyde solution for 30 mins ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL cell lysate was added to 3 mL of Ultima Gold scintillation liquid (Perkin Elmer #6013329). Samples were kept in the dark overnight and 3H-2DG uptake was measured the following day on a Hidex 300 SL scintillation counter ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mL of the cell lysate was counted in a liquid scintillation counter (TRI-CARB 4910TR, PerkinElmer) and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit ...
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was quantified by measuring luciferase activity in cell lysates using Britelite Plus (Perkin Elmer, Richmond, California) and following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant and the cell lysate were added into separate scintillation tubes containing Ultima Gold LSC cocktail (Perkin Elmer) and the radioactivity was measured by a TriCarb 3100TR liquid scintillation counter (Perkin Elmer) ...
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) and by real time PCR ...
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bioRxiv - Microbiology 2020Quote: ... Virus normalizations were performed by p24 ELISA according to the manufacturer’s instructions (Perkin Elmer) or by qPCR ...
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bioRxiv - Immunology 2021Quote: Vero-E6 were grown in DMEM supplemented with 10% FBS and seeded into clear bottom white 96 well plates (PerkinElmer, 6005688) at a density of 20’000 cells per well ...
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bioRxiv - Genomics 2019Quote: ... cell lysates were prepared with Passive Lysis Buffer and Luciferase activity was measured on a VICTOR X3 plate reader (PerkinElmer). Luciferase assay values are represented in fold change of Fluc (pGL4.23 plasmid ...
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bioRxiv - Immunology 2021Quote: ... cell lysate was loaded onto a 96-well plate (Grenier-BioOne) and mixed with MICROSCINTTM-20 scintillation fluid (Perkin Elmer). Samples were then incubated at room temperature for 2 hours and incorporated radioactivity analyzed on a 1450 MicroBeta TriLux Microplate Scintillation and Luminescence counter (Perkin Elmer).
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). Viral infectivity was considered positive if the RLU value was at least 5-fold higher than the background RLU value in the NP-2 parental cell line ...
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bioRxiv - Microbiology 2023Quote: ... The infectivity in each well was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer). The percentage of inhibition was determined by comparing the infectivity with positive control wells without drug inhibition.
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bioRxiv - Microbiology 2024Quote: ... and the infectivity was determined by measuring the RLUs in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infection was considered positive if the RLU value was more than 5-fold higher than the background RLU value in parental NP-2 cell line ...
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bioRxiv - Microbiology 2024Quote: ... The infectivity in each well was determined by measuring the RLU in the cell lysate using the Britelite plus system (PerkinElmer). The percentage of Maraviroc inhibition was determined by comparing the RLU values with the positive control wells without Maraviroc inhibition ...
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bioRxiv - Microbiology 2019Quote: ... WSN-GFP virus expression was detected by the Operetta-High Content Imaging System (PerkinElmer Inc.), and the percentage of GFP-positive cells were analyzed by Harmony4.1 software (PerkinElmer Inc.) ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was mixed with Optiphase Hisafe 3 (PerkinElmer), and radioisotope activity was monitored by LSC-8000 β-scintillation (Hitachi) ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was mixed with Ultima Gold (Perkin Elmer) and the relative expression of Na+-dependent uptake of radiolabeled glutamate was calculated based on the measurement with a microplate scintillation counter (1450 MicroBeta Plus ...
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bioRxiv - Microbiology 2021Quote: ... and the infectivity was determined by measuring the relative luciferase units (RLU) in the cell lysates using the Britelite plus system (PerkinElmer, USA). Viral infectivity was considered positive when the RLU value was at least 5-fold higher than the background RLU value in the parental NP-2 cell line ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA® SureFire® Ultra™ assay was run in total volume 20 μL (using only 10 μL cell culture lysate sample) in 384 well plate (PerkinElmer, #6007290) following standard assay two step no-wash protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Cholesterol efflux was determined as % counts per minute (CPM) in the supernatant versus cell lysates with a liquid scintillation analyzer (Tricarb-2810TR, Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Systems Biology 2021Quote: ... The His6-MBP-PDZ concentrations of soluble cell lysate fractions were evaluated with a microfluidic capillary electrophoretic system (Caliper LabChip GXII, PerkinElmer, Waltham, Massachusetts) and were adjusted to 4 μM by dilutions ...
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bioRxiv - Biochemistry 2023Quote: ... The other procedures were the same as the Zn transport assay except that the cell lysates were mixed with the Ultima Gold cocktail (PerkinElmer, Cat# L8286) and 55Fe associated with the cells was quantified using a liquid scintillation counter (LS 6500 Multi-Purpose Scintillation Counter).
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bioRxiv - Immunology 2020Quote: ... The virus infectivity titers were determined by measuring the luciferase activity using Britelite luciferase substrate (PerkinElmer Inc.) in a luminometer (Victor X2 ...
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bioRxiv - Microbiology 2022Quote: ... the virus was identified using liquid scintillation analyzer TRI-carb 2910 TR (Perkin Elmer, Waltham, Massachusetts, US). The virus containing fractions were collected and pelleted as earlier ...
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bioRxiv - Bioengineering 2021Quote: ... Concentrations of metals in the cell lysates were determined using inductively coupled plasma optical emission spectroscopy (ICPOES; Model Optima 4300D; Perkin Elmer, Norwalk, CT) operated at wavelengths of 238.204 and 259.939 nm for iron ...
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bioRxiv - Biochemistry 2021Quote: ... coli and all constructs were adjusted to approximately 4 μM concentration in cell lysate soluble fractions using a microcapillary electrophoretic system (Caliper; PerkinElmer, Waltham, MA, USA) and frozen in 96-well plates.