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Citations for PerkinElmer :
1 - 50 of 51 citations for Trypan Blue Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Cell growth was assayed using the standard trypan blue exclusion assay or ATPlite (Perkin Elmer). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... and the Opal dyes Opal 650 (PerkinElmer #2553339), Opal 570 (AKOYA Biosciences #OP-001003 ...
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bioRxiv - Neuroscience 2019Quote: ... Probes were fluorescently labeled with Opal Dyes (Perkin Elmer, Waltham ...
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bioRxiv - Neuroscience 2022Quote: ... probes were fluorescently labeled with Opal Dyes (Perkin Elmer) diluted in the TSA buffer ...
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bioRxiv - Pathology 2019Quote: ... Opal™ Dyes (NEL741E001KT, NEL744E001KT and NEL744E001KT, PerkinElmer, Waltham, MA) were used at 1:3500 in the TSA buffer provided in the RNAscope® Multiplex Fluorescent Kit v2 for each probe ...
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bioRxiv - Neuroscience 2021Quote: ... Probes were fluorescently labeled with Opal dyes (PerkinElmer, Waltham, MA). Opal dyes were diluted at 1:500 and assigned to each probe as follows ...
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bioRxiv - Developmental Biology 2019Quote: ... Probes were detected by Opal dyes 570/650 (Perkin Elmer). For subsequent antibody-based immunofluorescence ...
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bioRxiv - Molecular Biology 2022Quote: ... tyramide signal amplification and conjugation to an Opal dye (PerkinElmer) were performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained with zombie violet dye (BioLegend/Perkin Elmer) diluted 1:500 in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... near-infrared bisphosphonate-based calcium dye (OsteoSense 680, Perkin Elmer) for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Opal 570 dyes were purchased from Perkin Elmer (Akoya Biosciences), Nuclease-free water and PBS were purchased from Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: The near infrared fluorescent dye MMPsense 680 (PerkinElmer Inc, MA, USA) was used to monitor the MMP activity ...
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bioRxiv - Neuroscience 2022Quote: ... Probe labeling was done with Opal 570 dye (PerkinElmer, Inc NEL801001KT). Tiled images were acquired using a Zeiss LSM 880 with 10X and 40X objectives.
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bioRxiv - Neuroscience 2022Quote: ... Opal570 (red) and Opal 650 (far-red) fluorochrome dyes (Perkin Elmer) compared to DAPI staining (nuclei ...
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bioRxiv - Pathology 2023Quote: OsteoSense™ dye (OsteoSense 680 EX and NEV10020EX; PerkinElmer, MA, USA) was reconstituted in DPBS in a concentration of 20 nmol/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... mock or CAR T cells were labeled with Vivotrack-680 dye (PerkinElmer) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer) confocal screening microscope ...
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bioRxiv - Neuroscience 2020Quote: ... probes were fluorescently labeled with Opal Dyes (Perkin Elmer, Waltham, MA; 1:500) and stained with DAPI (4’,6-diamidino-2-phenylindole ...
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bioRxiv - Biochemistry 2022Quote: ... Formation of orange formazan dye was measured in EnVision plate reader (Perkin Elmer) at 560 nm ...
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bioRxiv - Genetics 2020Quote: ... green (XIST RNA probe) and blue (DAPI) fluorescent channels using the Openlab program (Perkin Elmer).
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then segmented based upon the nuclear dye using the Harmony software (PerkinElmer). Propidium iodide intensity levels were calculated and cells were classified as ‘dead’ if their intensity was above the established threshold.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 µL of Alamar Blue was dispensed with a FlexDrop precision dispenser (Perkin Elmer, Boston, MA). After an additional incubation of 24 hours ...
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bioRxiv - Biophysics 2022Quote: ... The dye and linker parameters were determined from the chemical structure using Chem3D 16.0 (PerkinElmer) and are summarized in Supp ...
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bioRxiv - Neuroscience 2023Quote: ... Probe signal was developed using Opal dyes (Opal 520 and 570 Reagent Pack, Perkin Elmer) at a dilution of 1:1000 and counterstained with DAPI ...
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bioRxiv - Developmental Biology 2022Quote: ... Opal dyes were used at a dilution of 1:750 (Perkin Elmer, Cat #FP1488001KT and #FP1495001KT). To multiplex with immunostaining ...
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bioRxiv - Microbiology 2023Quote: ... Absorbance of adhesion-retained CV dye was measured at 590 nm in Victor3 plate reader (PerkinElmer) and used as a measurement of adhesion to plastic surface.
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bioRxiv - Cancer Biology 2021Quote: ... For the Alamar Blue assay and fluorescence emission was read on an EnVision 2101 multilabel plate reader (PerkinElmer; excitation = 540 nm ...
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bioRxiv - Developmental Biology 2019Quote: ... tyramide signal amplification was performed according to the manufacturer’s protocol and conjugated to an Opal dye (PerkinElmer). The sections were treated with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: ... the cells were imaged confocally in blue and orange channels on a High Content Imaging System (Operetta CLS, PerkinElmer).
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bioRxiv - Cell Biology 2020Quote: ... The fluorescent intensity of equal amount mixture of oligonucleotide and PC dye solution was measured by EnSpire (PerkinElmer).
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bioRxiv - Bioengineering 2022Quote: ... and then 1 μM BeRST dye was added using a Janus Mini liquid handling robot (PerkinElmer, Waltham, MA). The cells were then incubated for 30 minutes at 30°C with no exposure to light ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... and the gel was stained with Coomassie Brilliant Blue (shown in grayscale in the figures) and incubated with EN3HANCE (PerkinElmer) solution ...
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bioRxiv - Neuroscience 2020Quote: ... In situ hybridization using nitro-blue tetrazolium and 5-bromo-4-chloro-3′-indolyphosphate and double color in situ hybridization using TSA Plus (PerkinElmer) were performed as previously described (Yamagata et al.,1999 ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Images of single stained tissues for each reagent were used to build spectral libraries of the single dyes by using the Nuance Multispectral Imaging System (Version 3.0.2, PerkinElmer Inc.). These spectral libraries were used to unmix the original multispectral images obtained with the Vectra imaging system (Suppl ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620 ...
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bioRxiv - Neuroscience 2021Quote: ... and the signal visualized through probe-specific HRP-based detection by tyramide signal amplification with Opal dyes (Opal 520, Opal 570 or Opal 690; Perkin Elmer) diluted 1:700 ...
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bioRxiv - Bioengineering 2020Quote: ... Images are acquired by confocal microscopy to minimize auto-fluorescence of unlabeled dye/antibody, using a conventional laser scanning confocal microscope (CLSM510Meta, Zeiss) or a high-content screening system (Opera Phenix, Perkin Elmer). Instrument specifications are found in Supplemental Information ...
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bioRxiv - Neuroscience 2021Quote: ... RNA-Targeting probes purchased from ACD Bio were then annealed at 40C for 2 hours followed by washing and a series of amplification steps before finally tagging the RNA with Opal Dye fluorophores (Perkin Elmer). In situ hybridization Images were captured on a Hamamatsu Nanozoomer S60 Digital Slide scanner with NDPview 2.0 software ...
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bioRxiv - Immunology 2022Quote: ... the ISH signal was amplified using a company-provided preamplifier and amplifier conjugated to fluorescent dyes (Perkin Elmer #NEL744001KT and #NEL745001KT) as described in the figure legends ...
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bioRxiv - Neuroscience 2023Quote: ... and the signal visualized through probe-specific horseradish-peroxidase-based detection by tyramide signal amplification with Opal dyes (Opal 520 and Opal 570; Perkin Elmer) diluted 1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... probes were fluorescently labeled with Opal Dyes (Opal 520 product no. FP1487001KT, Opal 570 product no. FP1488001KT, Opal 690 product no. FP1497001KT; Perkin Elmer). Autofluorescence from lipofuscin was quenched using the reagent TrueBlack (Biotium ...
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bioRxiv - Genetics 2019Quote: ... with the exception that the blue and green probes were labeled with coumarin-5-dUTP (custom synthesis, Perkin Elmer Life and Analytical Sciences) and fluorescein-12-dUTP (Perkin Elmer) ...
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bioRxiv - Bioengineering 2021Quote: Fluorescent PLGA particles were synthesised by encapsulating a near-IR dye (Cy7) to enable detection in a live animal using an in vivo imaging system (IVIS® Spectrum Perkin Elmer). Immediately after particle delivery ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 2% PFA at RT for 30 min and stained with the Hoechst 33342 dye (1 μg/ml) at 37 °C and 5% CO2 before imaging with the Operetta™ high content imaging system (PerkinElmer Inc.). Forty fields were acquired from each well using appropriate filter sets ...
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bioRxiv - Developmental Biology 2022Quote: ... Tyramide signal amplification reaction was performed using Cy3 fluorescent dye (diluted 1:50 using amplification diluent) (Perkin Elmer Life Sciences, Inc., NEL753001kt). The embryos were co-stained with CrebA and Crb antibodies for visualizing SG nuclei and apical cell boundaries ...
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bioRxiv - Developmental Biology 2021Quote: ... the RNAscope Multiplex Fluorescent Reagent Kit (v.2) and TSA Plus reagents (Perkin Elmer; 1:500 dilution for Cy3 and Cy5 dyes) or Opal dyes (Akoya Biosciences ...
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bioRxiv - Biophysics 2024Quote: ... samples were mounted upside down and cilia were live-stained with fluorescent-dye conjugated tomato lectin (IVISense™ Tomato Lectin 680, Perkin Elmer) by incubating the sample in a 0.25 µM dilution in PBS for 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620; FP1488001KT, Perkin Elmer, or Opal 690 ...
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bioRxiv - Developmental Biology 2024Quote: ... and/ or -C4 reagent for 15 min followed by incubation with an opal dye (Opal 570; FP1487001KT; Perkin Elmer, Opal 620; FP1488001KT, Perkin Elmer, or Opal 690; FP1497001KT; Perkin Elmer). The HRP signal was blocked by adding the RNAscope Multiplex FL V2 blocker for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Vectastain Elite ABC kit) followed by the tyramide signal amplification reaction (Cy3 fluorescent dye in amplification diluent; 1:50) (Perkin Elmer Life Sciences, NEL753001kt). To mark the SG and the apical membrane ...