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Citations for PerkinElmer :
1 - 50 of 744 citations for Thymidine Kinase 1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 1 mCi/ml 3H-Thymidine (PerkinElmer, NET027W005MC) in 2% ethanol was added directly to culture medium to a final concentration of 2 μCi/ml.
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bioRxiv - Immunology 2023Quote: ... cells were pulsed with 1 μCi [3H]-thymidine (PerkinElmer, #NET027A005MC) followed by collection on glass fiber filters (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... 0.001 mCi of [3H]-thymidine (PerkinElmer) was added per well and 18h later ...
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bioRxiv - Immunology 2021Quote: ... 0.001 mCi of [3H]-thymidine (PerkinElmer) was added per well and 22h later ...
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bioRxiv - Molecular Biology 2021Quote: ... transiently transfected INS-1 cells were incubated with 3H-thymidine (Perkin Elmer) at a final concentration of 1 Ci/ml in supplemented RPMI for 4 h ...
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bioRxiv - Immunology 2023Quote: ... measuring titrated thymidine incorporation (PerkinElmer, Boston, USA). Culture supernatants were analysed for cytokine expression using the Th1/Th17 panel from Luminex technologies according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... after incubation for 16 h with 1 µCi/ml [methyl-3H]-thymidine (Perkin Elmer). Data were expressed as counts per min (Cpm ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... with the last 8 hours in the presence of [3H] thymidine (1 mCi/well, NET027001MC; PerkinElmer). Incorporated [3H] thymidine was counted by a BetaPlate scintillation counter (Wallac ...
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bioRxiv - Immunology 2022Quote: ... 10,000 cells per well were seeded and incubated with 1 µCi/mL [3H]thymidine (Perkin Elmer) for specified time periods ...
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bioRxiv - Immunology 2020Quote: ... cells were incorporated with 3H-thymidine (Perkin Elmer, Hamburg, Germany) and incubated for 16 more hours ...
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bioRxiv - Immunology 2021Quote: ... Cells were then pulsed with 1μCi 3H-thymidine (Perkin Elmer, Waltham, MA) or 40μM BrdU for four hours at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The amount of 3H-thymidine incorporation was measured using the Filtermate harvester (PerkinElmer).
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bioRxiv - Immunology 2023Quote: ... and analysis of incorporated [3H]-thymidine in a ý-counter (PerkinElmer, #1450 MicroBeta TriLux).
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were harvested and [3H]-thymidine incorporation was determined with the 1450 MicroBeta TriLux (PerkinElmer), converting degree of radioactivity to counts per minute (cpm).
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bioRxiv - Immunology 2021Quote: ... Counts from 3H-thymidine incorporation were measured with a TopCount NXT Microplate Scintillation & Luminescence Counter (Perkin Elmer) and BrdU incorporation was measured on an LSR II or Symphony PRO flow cytometer (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... 3H-thymidine incorporation was measured using a 96 well Tomtec harvester and a Microbeta scintillation counter (PerkinElmer).
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bioRxiv - Cell Biology 2021Quote: AlphaLISA® SureFire® Ultra™ cellular kinase immunoassays were used to measure endogenous kinase activity of p-mTOR (Ser2448) (PerkinElmer, #ALSU-PMTOR-C500), p-ERK 1/2 (Thr202/Tyr204 ...
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bioRxiv - Microbiology 2021Quote: Culture supernatants were assayed for RT activity by surveying for 3H-thymidine incorporation (3H-TTP; Perkin Elmer #NET221A001MC). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Biochemistry 2024Quote: ... were transferred to 1.5 mL Eppendorf tubes containing 10 μL of pre-incubated at 37°C respective radioisotope (3H methionine [0.77 μCi, Perkin Elmer], 3H uridine [0.1 μCi, Perkin Elmer] or 3H thymidine [0.32 μCi, Perkin Elmer]). Concurrently ...
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bioRxiv - Microbiology 2024Quote: ... Of each sample three replicates and two formaldehyde-fixed (final conc. 1.85 %) adsorption blanks were spiked with [methyl-3H]-thymidine and L-[14C(U)]-leucine (Perkin Elmer) at saturating concentrations of 20 nM and 150 nM ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were radiolabelled with 32P-ATP by poly nucleotide kinase (PNK) (PerkinElmer PNK) for 1 hour at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 50 μL of a 1:2 dilution of SteadyLite Plus (Perkin Elmer, 6066759) in PBS with Ca++ and Mg++ was added to cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 6-24 h at 37 °C (as specified in each figure legend) the cell medium was aspirated and substituted with serum-free cell medium containing [3H]thymidine (2 μCi/ml, Perkin Elmer, Waltham, MA). The incubation was continued for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Immunology 2021Quote: ... The radioactive thymidine incorporated into the DNA of proliferating T cells was determined by liquid scintillation counting using a micro-β counter (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2019Quote: ... and protein production (BPL) were measured in triplicates by using the dual labeling with [metil-3H]-thymidine and [14C (U)]-leucine (PerkinElmer Inc., Waltham, MA, USA) of samples processed by the centrifugation method as described in Camarena-Gómez ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Biochemistry 2024Quote: ... HRP labelled goat anti-rabbit IgG (Perkin Elmer: NEF812001EA, 1:10,000), StarBright Blue 520 goat anti-mouse IgG (Bio-Rad 64456855 ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and a 1:200 dilution of anti-rabbit IgG acceptor bead (Perkin Elmer, AL104C) dissolved in 1X Alpha-LISA assay buffer (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated in HRP-conjugated goat anti-rabbit IgG (1:200; Perkin Elmer) for 30 minutes then incubated in Cy3 diluted in TSA amplification diluent (1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...