Labshake search

1 - 36 of 36 citations for TRAP 14 amide since 2019

• Competitive binding of MatP and topoisomerase IV to the MukB dimerization hinge

  Gemma L. M. Fisher, et al., bioRxiv - Biochemistry 2021

  Quote: ... Fluorescence arrival times were recorded on a SPQR-14 detector (PerkinElmer) and processed using custom software written in LabVIEW ...
• Structural basis of interdomain communication in PPARγ

  Sarah A. Mosure, et al., bioRxiv - Biochemistry 2022

  Quote: ... Photons were detected on four avalanche photodiodes (SPCM AQR 14, PerkinElmer Optoelectronics ...
• A bright and high-performance genetically encoded Ca²⁺ indicator based on mNeonGreen fluorescent protein

  Landon Zarowny, et al., bioRxiv - Molecular Biology 2020

  Quote: ... and detected by a fiber-coupled Avalanche Photodiode (APD) (SPCM_AQRH-14, Perkin Elmer). The obtained two photon excitation spectra were normalized for 1 μM concentration and further used to obtain the action cross-section spectra (AXS ...
• Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+

  Roger S. Zou, et al., bioRxiv - Bioengineering 2022

  Quote: ... Two avalanche photodiodes detect the fluorescent photons (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
• Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission

  Abhi Aggarwal, et al., bioRxiv - Neuroscience 2022

  Quote: ... and detected by a fiber-coupled Avalanche Photodiode (APD) (SPCM_AQRH-14, Perkin Elmer). The brightness spectra were normalized for 1 μM concentration and further used to obtain action cross-section spectra (AXS ...
• Ultrafast molecular dynamics observed within a dense protein condensate

  Nicola Galvanetto, et al., bioRxiv - Biophysics 2022

  Quote: ... followed by detection with SPCM-AQRH-14-TR single-photon avalanche diodes (PerkinElmer).
• Iron-loading is a prominent feature of activated microglia in Alzheimer’s disease patients

  Boyd Kenkhuis, et al., bioRxiv - Neuroscience 2021

  Quote: ... Solutions currently available for microglia cell segmentation (Abdolhoseini et al., 2019 [14], Inform, PerkinElmer) typically fall short of capturing the whole microglia area (Supplementary Fig ...
• Membrane compression by synaptic vesicle exocytosis triggers ultrafast endocytosis

  Haoyuan Jing, et al., bioRxiv - Cell Biology 2022

  Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
• The two zinc fingers in the nucleocapsid domain of the HIV-1 Gag precursor are equivalent for the interaction with the genomic RNA in the cytoplasm, but not for the recruitment of the complexes at the plasma membrane

  Serena Bernacchi, et al., bioRxiv - Biophysics 2020

  Quote: ... The fluorescence was directed to a fiber coupled APD (SPCM-AQR-14-FC, Perkin Elmer), which was connected to a time-correlated single photon counting module (SPC830 ...
• A combination of Interferon Stimulated Genes is more effective than IFNα and IFNβ in reducing HIV-1 replication in human cervicovaginal tissues

  Christiane Rollenhagen, et al., bioRxiv - Immunology 2019

  Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
• Intersectin and Endophilin condensates prime synaptic vesicles for release site replenishment

  Tyler Ogunmowo, et al., bioRxiv - Neuroscience 2023

  Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
• Dynamin 1xA interacts with Endophilin A1 via its spliced long C-terminus for ultrafast endocytosis

  Yuuta Imoto, et al., bioRxiv - Cell Biology 2023

  Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
• Control of Ca²⁺ signals by astrocyte nanoscale morphology at tripartite synapses

  Audrey Denizot, et al., bioRxiv - Neuroscience 2022

  Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
• Dengue virus 2 capsid protein chaperones strand displacement without altering the capsid-coding region hairpin element’s structural functionality

  Xin Ee Yong, et al., bioRxiv - Molecular Biology 2021

  Quote: ... The FAM donor fluorescence was recorded by a SPAD (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 513/17 band pass emission filter (Omega ...
• Simulation of calcium signaling in fine astrocytic processes: effect of spatial properties on spontaneous activity

  Denizot Audrey, et al., bioRxiv - Neuroscience 2019

  Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
• Dengue virus strain 2 capsid protein switches the annealing pathway and reduces the intrinsic dynamics of the conserved 5’ untranslated region

  Xin Ee Yong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The 5’FAM donor fluorescence was recorded by a SPAD (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 513/17 band pass emission filter (Omega ...
• Engineering Modular and Tunable Single Molecule Sensors by Decoupling Sensing from Signal Output

  Lennart Grabenhorst, et al., bioRxiv - Biophysics 2023

  Quote: ... USA for the green channel) and focused onto the single photon detectors (SPCM, AQR 14, PerkinElmer, USA). A custom LabVIEW program was used for data acquisition.
• Cargo crowding, stationary clusters and dynamical reservoirs in axonal transport

  Vinod Kumar, et al., bioRxiv - Biophysics 2021

  Quote: ... using the Hamamatsu ImagEM C9100-13/14 EMCCD Camera integrated with an Olympus IX83 microscope by Perkin Elmer. Samples were imaged using a 100X 1.63N.A ...
• Selective G protein signaling driven by Substance P-Neurokinin Receptor structural dynamics

  Julian A. Harris, et al., bioRxiv - Biochemistry 2021

  Quote: ... pH 7.0) and 14 μl of lysis were added to wells of a 384 well white proxiplate (PerkinElmer) for analysis ...
• Clustering-mediated activation of Cdc42 GTPase antagonized by GAPs in fission yeast

  Iker Lamas, Nathalie Weber, Sophie G Martin, bioRxiv - Cell Biology 2020

  Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the CRY2-Cdc42ΔCaaX and/or CRY2 systems ...
• Fast and sensitive GCaMP calcium indicators for imaging neural populations

  Yan Zhang, et al., bioRxiv - Neuroscience 2021

  Quote: ... Semrock) and a bandpass filter (550BP200, Semrock) and detected by a fiber-coupled Avalanche Photodiode (APD) (SPCM_AQRH-14, Perkin Elmer). The obtained two-photon excitation spectra were normalized to 1 μM concentration and subsequently used to obtain the action cross-section spectra (AXS ...
• DNA sequence-dependent positioning of the linker histone in a nucleosome: a single-pair FRET study

  Madhura De, et al., bioRxiv - Biophysics 2021

  Quote: ... to donor and acceptor signals which are sent through filters (donor channel: 520df40, acceptor channel: 610ALP, Omega Optical) to two avalanche photodiodes (SPAD-AQ-14, PerkinElmer Optoelectronics ...
• Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase

  Iker Lamas, et al., bioRxiv - Cell Biology 2019

  Quote: ... solid-state laser lines and a cooled 14-bit frame transfer EMCCD C9100-50 camera (Hamamatsu) and is run by Volocity (PerkinElmer). When imaging strains expressing the OptoQ61L and/or Opto systems ...
• Glutamate helps unmask the differences in driving forces for phase separation versus clustering of FET family proteins in sub-saturated solutions

  Mrityunjoy Kar, et al., bioRxiv - Biophysics 2023

  Quote: ... the setup is equipped with a 488-nm laser line (Cobolt 06-MLD) for excitation of EGFP fluorophores and a single-photon counting avalanche photo diode (SPCM-14, PerkinElmer) for subsequent detection of emitted fluorescence photons ...
• Cargo crowding, stationary clusters and dynamical reservoirs in axonal transport

  Vinod Kumar, et al., bioRxiv - Biophysics 2021

  Quote: ... using a 100X/1.4 NA oil objective on the Olympus IX83 microscope integrated with the Yokogawa CSU-X1-A3 spinning disc and Hamamatsu ImagEM C9100-13/14 EMCCD Camera (by Perkin Elmer). The analysis for vesicle accumulation following axotomy was conducted as follows-
• Disordered Proteins Enable Histone Chaperoning on the Nucleosome

  Pétur O. Heidarsson, et al., bioRxiv - Biophysics 2020

  Quote: ... acceptor emission was filtered with a LP647RU long-pass filter (Chroma) and detected with a SPCM-AQRH-14 single-photon avalanche diode (Perkin Elmer). After surface immobilization of nucleosomes ...
• Supra-molecular organization of the pyoverdine bio-synthetic pathway in Pseudomonas aeruginosa

  Véronique Gasser, et al., bioRxiv - Biophysics 2019

  Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
• 2-photon-fabricated nano-fluidic traps for extended detection of single macromolecules and colloids in solution

  Oliver Vanderpoorten, et al., bioRxiv - Biophysics 2021

  Quote: ... The emitted photons were filtered through a band-pass filter (FF01-520/35-25, Semrock) and then focussed onto an avalanche photodiode (APD, SPCM-14, PerkinElmer Optoelectronics) connected to a TimeHarp260 time-correlated single-photon counting unit (PicoQuant) ...
• Direct digital sensing of protein biomarkers in solution

  Georg Krainer, et al., bioRxiv - Biophysics 2023

  Quote: ... The emitted photons are filtered through a band-pass filter (FF01-520/35-25, Semrock) and then focused onto a single-photon counting avalanche photodiode (APD, SPCM-14, PerkinElmer Optoelectronics), which is connected to a TimeHarp260 time-correlated single photon counting unit (PicoQuant).
• Single-molecule digital sizing of proteins in solution

  Georg Krainer, et al., bioRxiv - Biophysics 2023

  Quote: ... The emitted photons are filtered through a band-pass filter (FF01-520/35-25, Semrock) and then focused onto a single-photon counting avalanche photodiode (APD, SPCM-14, PerkinElmer Optoelectronics), which is connected to a TimeHarp260 time-correlated single photon counting unit (PicoQuant).
• PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa

  Hanna Manko, et al., bioRxiv - Microbiology 2023

  Quote: ... and a 525/50 nm band-pass filter (F37-516, AHF, Germany) and directed to a fibre-coupled avalanche photo-diode (SPCM-AQR-14-FC, Perkin Elmer) connected to a time-correlated single photon counting (TCSPC ...
• The Hsc70 Disaggregation Machinery Removes Monomer Units Directly from α-Synuclein Fibril Ends

  Matthias M. Schneider, et al., bioRxiv - Biophysics 2020

  Quote: ... the microscope is equipped with a 488-nm laser line (Cobolt 06-MLD, Hübner Photonics, Derby, UK) and a single-photon counting avalanche photo diode (SPCM-14, PerkinElmer, Seer Green, UK) for subsequent detection of emitted fluorescence photons ...
• Addressable Nanoantennas with Cleared Hotspots for Single-Molecule Detection on a Portable Smartphone Microscope

  Kateryna Trofymchuk, et al., bioRxiv - Biophysics 2020

  Quote: ... The light was later focused on a 50 µm pinhole (Linos AG, Germany) and detected using Single-Photon Avalanche Diodes (SPCM, AQR 14, PerkinElmer, Inc., USA) registered by an TCSPC system (HydraHarp 400 ...
• Dengue virus strain 2 capsid protein switches the annealing pathway and reduces the intrinsic dynamics of the conserved 5’ untranslated region

  Xin Ee Yong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The donor and acceptor fluorescence were recorded using a set of single-molecule avalanche photodiodes (SPADs) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 513/17 and 615/45 band pass emission filter (Omega ...
• Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state

  Anne-Marinette Cao, et al., bioRxiv - Biophysics 2021

  Quote: ... We use two MPD-1CTC (MPD, Bolzano, Italy) for the donor wavelength channels and two SPCM AQR-14 (Perkin Elmer, Fremont, CA, USA) for the acceptor wavelength channels ...
• Dengue virus strain 2 capsid protein switches the annealing pathway and reduces the intrinsic dynamics of the conserved 5’ untranslated region

  Xin Ee Yong, et al., bioRxiv - Molecular Biology 2020

  Quote: ... The 3’TAMRA fluorescence from 5’UAR was recorded by a single molecule avalanche photodiode (SPAD) (SPCM-AQR-14, PerkinElmer Optoelectronics, Quebec, Canada), through a 600/50 band pass emission filter ...