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Citations for PerkinElmer :
1 - 50 of 62 citations for Strong Cation Exchange Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mechanism of the ANT-mediated transport of fatty acid anions across the inner mitochondrial membranebioRxiv - Biophysics 2022Quote: ANT1-mediated exchange of ATP/ADP was measured radioactively using 3H-ATP (Perkin Elmer Prod.# NET420250UC) 67 ...
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bioRxiv - Biochemistry 2022Quote: Nucleotide exchange assays were carried out at 20 °C using a LS55 luminescence spectrometer (Perkin Elmer) with 5 nm slit widths (Ex/Em 295 nm/345 nm) ...
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bioRxiv - Physiology 2020Quote: ... media was replaced with media plus tracer (i.e. L-14C-U-Leucine, NEC279E0, Perkin Elmer) plus 10 nM insulin (91077C ...
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bioRxiv - Biochemistry 2023Quote: ... BRB80 was mixed with a 1/10 volume of 10X nucleotide exchange buffer (10 mM GTP, 30 nM GTP α-32P (PerkinElmer BLU506H250UC), 1 mM nocodazole ...
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bioRxiv - Cell Biology 2021Quote: ... Media were replaced with fresh media supplemented with Easytag Express S35 Protein Labeling Mix (PerkinElmer, 100-200 µCi/mL) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... cells resuspended in 2ml of fresh media and 40ul of media was plated per well of a black 384-well CellCarrierUltra (PerkinElmer). Cells were left for 48h to release ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 200 μM ascorbate and 100 mCi/mL of 35S-Cys/Met (Perkin Elmer) for 10 min ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... Media samples were added to 96-well white optiplates (PerkinElmer) in duplicate ...
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bioRxiv - Biophysics 2021Quote: ... media was removed and 100 μl Delfia Enhancement solution (PerkinElmer) was added to each well ...
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bioRxiv - Physiology 2023Quote: ... Glucose-free media (500 μl) containing 1μCi/ml [14C] glucose (Perkin Elmer) were added to cells in the absence or presence of phlorizin (100 μM ...
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bioRxiv - Cell Biology 2021Quote: ... to prevent media evaporation and cells were imaged on the Operetta CLS (PerkinElmer) at 37 °C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... culture media were supplemented ON with 1 mCi 14C-glucose (Perkin Elmer #NEC042V250UC). Cells were then washed 3 times in ice cold PBS and lysed in RIPA buffer (Thermo Fisher Scientific #89900) ...
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bioRxiv - Physiology 2021Quote: ... Subsequently media was supplemented with 250 μCi of [35S]-cysteine/methionine mixture (PerkinElmer, NEG772002MC) and islets were incubated under normal conditions for 30 min ...
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bioRxiv - Physiology 2021Quote: ... Subsequently media was supplemented with 250 μCi of [35S]-cysteine/methionine mixture (PerkinElmer, NEG772002MC) and cells incubated under normal conditions for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... Virus released from cells was quantified by media-associated p24 determined by ELISA (PerkinElmer) and equivalent amounts of p24.
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bioRxiv - Neuroscience 2023Quote: ... Media was assayed using an AlphaLISA assay for Aβ1-42 (Perkin Elmer catalog # AL276C), sAPPα (R&D Systems catalog # AF1168 conjugated with Perkin Elmer acceptor beads catalog # 6772001 + 2B3 antibody from IBL catalog # 11088 biotinylated) ...
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bioRxiv - Cell Biology 2019Quote: ... Activity in media and cell fractions was measured using a Wizard 2480 automated gamma counter (PerkinElmer), and the % activity retained calculated as the percentage of the total amount in the cell+media fractions in the cell fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 cells in phenol-red free media were plated per well of a 384 CellCarrierUltra plate (PerkinElmer) the day before imaging ...
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bioRxiv - Bioengineering 2021Quote: ... Media was then sampled and fluorescence at 560 nm was analysed using VICTOR Multilabel Plate Reader (Perkin Elmer). Media containing PrestoBlue incubated in empty wells was used as background control.
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bioRxiv - Cancer Biology 2023Quote: ... After the addition of recording media plates were sealed with TopSeal-A Plus plate covers (6050185, Perkin Elmer).
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bioRxiv - Cancer Biology 2023Quote: ... After the addition of recording media plates were sealed with TopSeal-A Plus plate covers (6050185, Perkin Elmer). All cells were maintained at 37°C and 5% CO2 and were confirmed to be mycoplasma-free.
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bioRxiv - Immunology 2022Quote: 50,000 cells per well were seeded and allowed to attach before gentle washing and incubation for 48 hours in media containing 0.4 µCi/mL (24.24 nM final concentration) [5-3H]glucose (Perkin Elmer) or 2 µCi/mL (66.67 nM final concentration ...
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bioRxiv - Cell Biology 2022Quote: hTert-RPE1 Ruby-PCNA or hTert-RPE1 Ruby-PCNA p21-GFP H3.1-iRFP cells were seeded at a density of 2.5×104 cells/ml in 20 μl of media/well in 384-well CellCarrier Ultra plates (PerkinElmer) or 8-well Ibidi chambers one day before adding inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... normalized by cell OD600 (final OD=0.4) and diluted 1: 100 into 150 μl of fresh TSB media in flat-bottomed 96-well plates (PerkinElmer). The cultures were overlaid with 100 μl of mineral oil (Sigma ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol-free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 30min of incubation at 37°C supernatants were collected ...
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bioRxiv - Cell Biology 2023Quote: ... pulse–chase experiments were performed in labelling media supplemented with 2.5 μCi/ml [14C]proline (Perkin Elmer, Waltham, USA). After incubation in supplemented labelling media for 18 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... arrays were explanted from the mouse host and immediately incubated with cell culture media containing D-luciferin (0.15 mg/mL; PerkinElmer) for 10 minutes and then imaged using the In Vivo Imaging System (IVIS ...
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bioRxiv - Immunology 2023Quote: ... 5x104 of cells were resuspended in 150ul of phenol free RPMI media and spiked with 20uM of ATP (PerkinElmer). After 10min of incubation at 37°C supernatants were collected and concentration of ATP/sample was measured using ATPlite Luminescence Assay System (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... 100uL of media was removed from the plate and incubated with 100 uL Britelite Plus reagent (PerkinElmer, Waltham, MA) for 2 minutes followed by mixing and transferring to a black 96 well assay plate ...
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bioRxiv - Cell Biology 2023Quote: ... Media was replaced for a fresh aliquot and cells were imaged using Opera Phenix High Content Screening System (PerkinElmer) every 6min20sec for 8h in a transmitted light channel ...
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bioRxiv - Microbiology 2023Quote: ... was added to the media at a 1:10,000 dilution and images were acquired using the Opera Phenix High-Content Screening System (PerkinElmer). Analysis was performed using Harmony 191 High-Content Imaging and Analysis Software (PerkinElmer ...
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bioRxiv - Microbiology 2024Quote: Three independent overnight cultures of each strain grown in LB media were back diluted in 200 µl DSM media to 0.1 OD600 in a 96 well flat-bottom microplate (PerkinElmer). The plate was then incubated at 37 °C with continuous shaking (600 cpm ...
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bioRxiv - Microbiology 2024Quote: ... three independent overnight cultures of the spo0A-gfp and the ΔshfA ΔshfP spo0A-gfp reporter strains in LB media were back diluted in 200 μl DSM media to 0.1 OD600 in a 96-black walls and clear bottom wells microplate (PerkinElmer). The plate was then incubated at 37 °C with continuous shaking (600 cpm ...
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bioRxiv - Molecular Biology 2019Quote: Cells were incubated with complete growth media supplementing with EasyTagTM Express Protein Labeling Mix containing both [35S]-L-methionine and [35S]-L-cysteine (PerkinElmer) at 10 μCi/ml for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular culture media were collected in order to dose secreted Aβ using Alpha-LISA assays (AlphaLISA Amyloid β1-X Kit, AL288C, PerkinElmer) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... We verified luciferase expression in selected stable cells after providing 150 ug/ml D-luciferin to the growth media and detecting bioluminescence using an IVIS In Vivo Imaging System (PerkinElmer).
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bioRxiv - Cancer Biology 2020Quote: ... 5uL of 8x concentrated compound solution (diluted in MCF10A complete growth media) was added into each well with a JANUS automated liquid handling system (PerkinElmer). At the time of drug treatment ...
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bioRxiv - Cell Biology 2021Quote: ... The AlphaLISA™ assay was in 20 μL reactions (requiring only 2 μL cell culture media sample) in 384-well plate (6007290, PerkinElmer) following standard two-step no-wash protocol without any modifications ...
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bioRxiv - Genetics 2022Quote: ... 7.5×104 cells in supplemented McCoy’s 5A media −/+ 500 µM auxin were seeded in 384-well plates (Perkin Elmer 6057300) and incubated at 37°C for 6 h ...
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bioRxiv - Synthetic Biology 2019Quote: ... 190 µl of each strain and of the media was placed in wells of a 96-well plate (Perkin Elmer). The negative feedback strain was also placed in other wells where following final concentrations of inducer aTc (in µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured in α-MEM containing media containing 2μM Glutamine and 4 mCi/mL L-[3,4-3H(N)]-Glutamine (PerkinElmer, NET551250UC). GLS activity was terminated by washing cells with ice cold HBSS for three times followed by scaping cells with 1 mL ice cold milliQ water ...
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bioRxiv - Microbiology 2023Quote: ... media was removed and replaced with 50μl DMEM without phenol red and 25μl of Britelite plus (Perkin Elmer, Richmond, California), and luciferase activity was measured following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... On the next day transfer and mix of compounds into plates only containing cell culture media was performed with the Sciclone G3 Liquid Handling Workstation (PerkinElmer). This intermediate dilution step was included to avoid DMSO gradient effects on cell monolayers ...
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bioRxiv - Biochemistry 2023Quote: cKO cells grown in the presence or absence of acetamide normalized to OD600 = 1 were incubated for 2 hours at 37 °C in 7H9 media containing [14C]-acetate (final concentration 0.5 μCi/ml; Perkin Elmer). Labeled cells were harvested by centrifugation at 5,000 x g for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Radioactivity in aliquots of media were determined by scintillation counting in a Beckman Coulter LS6500 Scintillation Counter using LSC Cocktail (PerkinElmer). Cell lysates were produced by addition of 0.1N NaOH for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µl of pre-made compound solution in media was added to each well and luciferase measurements were performed on an EnVision plate reader (PerkinElmer).
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bioRxiv - Cell Biology 2022Quote: ... An equal volume of cell culture media and esterase substrate (100 μM in esterase assay media at pH 5) was transferred to a black walled clear bottomed plate (Perkin Elmer) and fluorescence was measured after 1 h.
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bioRxiv - Immunology 2021Quote: ... For neutralization assays 104 293T-hACE2 cells were plated in 100uL media per well in 96 well white-wall white-bottom plates (Perkin Elmer) and incubated overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: MCF10A cells transfected with control or ErbB3 siRNA were incubated with growth factor deprived DMEM media lacking methionine and cysteine supplemented with 10 μCi/ml 35S-methionine/cysteine (Perkin Elmer) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 4,000 cells/well (for G2/M phase nocodazole treatment) with a complete media overnight onto poly-D-lysine-coated CellCarrier-Ultra-96 well Black (Perkin Elmer).