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Citations for PerkinElmer :
1 - 50 of 343 citations for SARS CoV 2 Spike Glycoprotein S1 RBD Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 pseudovirus productions were quantified by p24 ELISA (Perkin Elmer Life Sciences).
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bioRxiv - Microbiology 2020Quote: ... Calu-3 or Caco-2 cells were seeded and infected with SARS-CoV-2 in Optical 96-well plates (CellCarrier Ultra, PerkinElmer) and cells were fixed with 4% PFA at the indicated timepoints ...
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bioRxiv - Immunology 2021Quote: ... brain and testis tissue samples from hACE2 transgenic mice challenged intranasally with SARS-CoV-2-nLuc (1 x 105 FFU; 6 dpi) were imaged after necropsy using bioluminescence imaging (IVIS, Perkin Elmer), pruned to isolate regions with high nLuc activity and immediately pre-fixed with 3 % glutaraldehyde ...
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bioRxiv - Bioengineering 2023Quote: ... for SARS-CoV-2 S protein neutralization antibody titer in the wells was observed using the High Content Analysis System (Perkin Elmer, Waltham, MA, USA) or microscope ...
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bioRxiv - Genomics 2022Quote: SARS-CoV-2 genome libraries were constructed by using the NEXTFLEX® Variant-Seq™ SARS-CoV-2 kit with the automated high-throughput liquid handler Sciclone® G3 NGS (Perkin Elmer, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... The CHO-K1 cell line (PerkinElmer, ES-000-A24) was used for receptor activation assays ...
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bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were seeded to 96-well plates (PerkinElmer) coated with Fibronectin (Corning ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Neuroscience 2022Quote: GPCRs were heterologously expressed in CHO-K1 cells (Perkin Elmer, ES-000-A24), stably expressing mitochondrially tagged apo-aequorin and the promiscuous human Gα16 subunit ...
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bioRxiv - Neuroscience 2022Quote: ... and kappa (#ES-541-C) opioid-expressing CHO cell lines from PerkinElmer (Waltham, MA) were used ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Bioengineering 2024Quote: ... Vivo-Tag 680 XL (PerkinElmer #NEV11119) was used as a fluorescent dye to track idLFA1 and control murine serum albumin (MSA ...
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bioRxiv - Neuroscience 2020Quote: ... mammalian CHO K1 cells stably overexpressing apo-aequorin and human Gα16 (ES-000-A24, PerkinElmer) were transiently transfected with Ce-npr-4a/pcDNA3.1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing the human CB1 receptor were obtained from PerkinElmer (#ES-110-C) and utilized for western blots (CB1-CHO-C2 ...
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bioRxiv - Biochemistry 2024Quote: ... CHO-K1 cells were plated at 50,000/well in white/clear bottom 96 well Isoplates (PerkinElmer) in growth media a day before the experiments ...
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bioRxiv - Molecular Biology 2022Quote: CHO-K1 cells were seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The WT GIPR or SVs were transiently transfected using Lipofectamine 2000 transfection reagent ...
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bioRxiv - Cell Biology 2019Quote: ... Easy-Tag [35S]-Cys/Met was purchased from PerkinElmer Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Flp-In-CHO cells either stably or transiently expressing the M2/M5 mAChR chimeras were seeded in 96-well Isoplates (PerkinElmer Life Sciences) at a concentration of 20,000 – 25,000 cells per well a day before the experiment was performed in a humidified atmosphere at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibody to adiponectin (Table S1) was visualized with Western Lightning Plus (Perkin Elmer, Waltham, Massachusetts) and imaged using a LI-COR Odyssey Imager (LI-COR Biosciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... S1 were extracted from bioluminescence imaging data captured with an IVIS Lumina Series III (Perkin Elmer, Waltham, MA) and analyzed with Living Image 4.5.2 ...
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bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
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bioRxiv - Microbiology 2021Quote: ... 1.37 μg/mL of RBD and 5 μg/mL of Ecto) and incubated overnight in protein-binding 96-well plates (PerkinElmer). The next day ...
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bioRxiv - Molecular Biology 2023Quote: ... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Bioengineering 2022Quote: ... Two avalanche photodiodes detect the fluorescent photons (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Molecular Biology 2019Quote: ... Two probes were amplified from a plasmid template targeting the pBac arms (see Table S1 for primers) and labelled with CTP α-32P 3000Ci/mmol (Perkin Elmer) using Klenow Enzyme (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... (Figure S1) Its chemical structure was confirmed by 1H-NMR (Mercury 400, Varian, Palo Alto) and FTIR (Spectrum One, Perkin Elmer, Waltham) shown in Figure S2.
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
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bioRxiv - Cell Biology 2022Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Biophysics 2020Quote: ... The fluorescence was directed to a fiber coupled APD (SPCM-AQR-14-FC, Perkin Elmer), which was connected to a time-correlated single photon counting module (SPC830 ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2023Quote: ... The fluorescent photons are detected by two avalanche photodiodes (SPCM-AQR-14-FC, Perkin Elmer). The images are obtained by scanning a piezo-controlled stage (Max311D ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Neuroscience 2019Quote: ... The fluorescence signal was confocally detected by an avalanche photodiode (APD; SPCM-AQRH-14-FC; PerkinElmer). The spatial resolution of the setup was around 175 nm (in x-y ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Bioengineering 2021Quote: ... we used the TTL signal from an avalanche photo diode (APD, SPCM-AQRH-13-FC, Perkin Elmer). We illuminated the APD with natural light ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Neuroscience 2022Quote: ... The medium was removed and cells were incubated with 500 nM of SNAP-Red in Tag-Lite Buffer (Perkin Elmer Cisbio) for 1 hr at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and imaged onto the active area of two fibre-coupled avalanche photodiode detectors (APDs; Perkin Elmer, SPCM-AQRH-15-FC) using a cubic non-polarizing beam-splitter (Linos ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).