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Citations for PerkinElmer :
1 - 50 of 250 citations for Recombinant Zebrafish tnfa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: AlphaLISA assay kits (#AL204 for insulin, AL521 for Leptin and AL505 for TNFa) from PerkinElmer (Waltham, MA) and an Envision 2104 plate reader from the same supplier were used to assess plasma levels of the corresponding analyte using white 96-well microplates ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... zebrafish were anaesthesised and imaged on an Opera Phenix® High-Content Screening System (Perkin Elmer). The 96-wells were first imaged at 5x magnification to identify zebrafish via their GFP intensity ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
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bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by enhanced chemiluminescence (PerkinElmer, cat# NEL105001EA). Figures show representative images of 3-5 biological replicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... gravity-flow protein purification with Lipidex 1000 (Perkin Elmer) chromatography was conducted ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with Western Lightning Plus substrate (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: Proteins were directly radioiodinated with [125I]NaI (Perkin Elmer) using Pierce iodination reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer) and Anti-FLAG Alpha Donor Beads (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... Proteins were subsequently blotted onto PROTRAN Pure nitrocellulose membrane (PerkinElmer) using transfer buffer (25mM TRIS ...
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bioRxiv - Microbiology 2021Quote: ... proteins were detected with WesternLightning ECL Reagent (Perkin Elmer, Germany) and exposure of the membrane to CL-XPosure film (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were revealed using Lightning® Plus ECL (Perkin Elmer) and the bands were analysed by densitometry using ImageJ (NIH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein signal was detected using enhanced chemiluminescence (ECL) reagent (PerkinElmer). The antibodies used were obtained from commercial sources ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... and 1 µL Express 35S protein labelling mix (Perkin Elmer) in the presence of 0.1% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... And proteins were visualized using enhanced chemiluminescence detection (NEL103E001EA, PerkinElmer) by AI600 (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.08 μl of Protein A-coated acceptor beads (PerkinElmer) in AlphaScreen buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.08 μl of Protein A-coated acceptor beads (PerkinElmer) in AlphaScreen buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 of EasyTagTM EXPRESS 35S Protein Labeling Mix (Perkin Elmer) (5.5 μCi ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using Western Lightning ECL Plus (Perkin Elmer) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg/mL AlphaLISA protein-A acceptors beads (PerkinElmer #AL101) and 10 µg/mL donor beads were added ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... 20 µCi/mL EasyTag Express 35S Protein Labeling Mix (PerkinElmer) was added to cultures and temperature was reduced to 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with Western Lightning ECL Pro substrate (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then transferred to a PVDF membrane (Perkin Elmer) for 90 min with a semidry blotter (Peqlab ...
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bioRxiv - Neuroscience 2020Quote: ... Protein signals were developed by using ECL kit (NEL103001EA, Perkin Elmer). Quantification of interested protein bands were conducted by using NIH Image J software ...
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bioRxiv - Cell Biology 2020Quote: ... and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... protein expression levels were detected with Western Lightening Ultra (Perkin Elmer) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...