Labshake search
Citations for PerkinElmer :
1 - 50 of 412 citations for Recombinant Human Interleukin 2 Receptor Alpha His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
-
bioRxiv - Neuroscience 2023Quote: ... Alpha-LISA kits specific for human Aβ1–X (AL288C, PerkinElmer) and Aβ1–42 (AL276C ...
-
bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... CHO cells stably expressing the human CB1 receptor were obtained from PerkinElmer (#ES-110-C) and utilized for western blots (CB1-CHO-C2 ...
-
bioRxiv - Genomics 2021Quote: ... Insulin was quantified using the Insulin (human) AlphaLISA Detection Kit and the EnSpire Alpha Plate Reader (both Perkin Elmer) based on 1:10 and 1:200 dilutions for supernatant and insulin content ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Radioligand binding assays were performed with membranes prepared from cells expressing human CB2 or CB1 or mouse CB2 receptor using [3H]-CP55940 (Perkin Elmer) as a radioligand ...
-
bioRxiv - Neuroscience 2019Quote: ... and alpha-35S-UTP (PerkinElmer #NEG039H250UC). Brain sections were postfixed in 4% paraformaldehyde for 1 h and acetylated for 10 min with 0.1M triethanolamine hydrochloride/0.25% acetic anhydride (pH 8.0) ...
-
bioRxiv - Cell Biology 2022Quote: ... and alpha-32P-dCTP (Perkin Elmer). Templates were produced by PCR using the following primers (RNR4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the Alpha Screen cAMP assay kit (PerkinElmer) was used according to the manufacturer’s protocol ...
-
bioRxiv - Pathology 2019Quote: ... ALPHA-[32P] / [32P] TTP (Perkin Elmer BLU005A250MC). Samples were incubated at 37° C overnight ...
-
bioRxiv - Synthetic Biology 2024Quote: ... anti-FLAG Alpha Donor beads (Perkin Elmer) were used to immobilize the sFLAG tagged peptides and anti-Maltose-Binding (MBP ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Anti-FLAG Alpha Donor Beads (PerkinElmer) to 80 µg/mL in 0.2% BSA/PBS ...
-
bioRxiv - Neuroscience 2019Quote: ... and labeled with alpha-35S-CTP (PerkinElmer #NEG064H250UC) and alpha-35S-UTP (PerkinElmer #NEG039H250UC) ...
-
bioRxiv - Microbiology 2021Quote: ... Strep-Tactin® Alpha donor beads (PerkinElmer, AS106D) and nickel chelate (Ni-NTA ...
-
bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
-
bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
-
bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
-
bioRxiv - Cell Biology 2022Quote: ... IVISense Integrin Receptor (IntegriSenseTM) 645 (PerkinElmer, 1/300 dilution). Following primary antibody incubation ...
-
bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
bioRxiv - Cancer Biology 2019Quote: ... Luminescence was measured on an EnSpire Alpha (Perkin Elmer) using the integrated software package ...
-
bioRxiv - Molecular Biology 2022Quote: ... The Alpha signal was detected on Envision Readers (PerkinElmer). The same amount of DMSO was used as the negative control ...
-
bioRxiv - Neuroscience 2023Quote: ... Luminescence was measured using an EnVision-Alpha Reader (PerkinElmer) at 680-nm excitation and 615-nm emission wavelengths.
-
bioRxiv - Cancer Biology 2023Quote: ... After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer) and Anti-FLAG Alpha Donor Beads (PerkinElmer ...
-
bioRxiv - Cancer Biology 2023Quote: ... and the plate was incubated in the dark for 2 h and then read in an EnSpire Alpha multimode plate reader (PerkinElmer, Waltham, MA, USA).
-
bioRxiv - Microbiology 2021Quote: ... and nickel chelate (Ni-NTA) Alpha acceptor beads (PerkinElmer, 6760619C) were pre-mixed and added to the reaction at a final concentration of 10 μg/mL ...
-
bioRxiv - Cell Biology 2022Quote: ... dissolved in 1X Alpha-LISA assay buffer (Perkin Elmer, AL000F). Plates read by laser irradiation of donor beads at 680 nm generating chemiluminescent emission from the acceptor bead at 615 nm using EnVision Multimode Plate Reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2023Quote: ... and were added into a 384-well Alpha plate (PerkinElmer). Compounds were serially diluted in the same buffer containing 1% DMSO ...
-
bioRxiv - Molecular Biology 2024Quote: ... Alpha signal was detected using Enspire plate reader instrument (PerkinElmer). The raw Alpha counts were normalized to DMSO-treated controls within every plate.
-
bioRxiv - Biochemistry 2024Quote: ... The signal was measured using an EnSpire Alpha (PerkinElmer, Inc.) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
-
bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Genomics 2021Quote: Two-color FISH experiments were performed using human fosmid (n=2) or BAC (n=2) clones directly labelled by nick-translation with Cy3-dUTP (PerkinElmer) and fluorescein-dUTP (Enzo ...
-
bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
-
bioRxiv - Microbiology 2020Quote: ... and acceptor antibodies were conjugated to Alpha Acceptor Beads (PerkinElmer #6760137M).
-
bioRxiv - Developmental Biology 2020Quote: ... the plate was read with an EnSpire Alpha plate reader (PerkinElmer). The standard curve was calculated with GraphPad Prism software (San Diego ...
-
bioRxiv - Immunology 2022Quote: ... and interleukin 10 (IL-10) were quantified in the supernatant from the PBMCs using an AlphaLISA assay (PerkinElmer, Waltham, MA). AlphaPlates containing 2 µL of supernatant were thawed at 25°C for 15 minutes ...
-
bioRxiv - Microbiology 2023Quote: ... The assay was measured with an EnVison-Alpha plate reader (Perkin Elmer) and analysed via the standard curve.
-
bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
-
bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
-
bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
-
bioRxiv - Biochemistry 2023Quote: Endogenously tagged HiBiT cells were plated in 96-well plates (PerkinElmer) at a density of 0.5×106 cells/mL ...
-
bioRxiv - Molecular Biology 2021Quote: ... was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer, Cat nr. 6760002). Human ACE-2-mFc protein (Sino Biological ...
-
bioRxiv - Molecular Biology 2024Quote: ... All the components were diluted in the Alpha Screen Control Buffer (Perkin Elmer). The signal was detected using an EnSight® multimode plate reader (PerkinElmer ...
-
bioRxiv - Biochemistry 2024Quote: ... Acceptor mixtures were prepared by mixing ULight alpha-6xHIS acceptor antibody (Perkin Elmer) with 6xHIS-tagged Swc5 variants at a ratio of 1:20 and serially diluting across 13 concentrations in H66 buffer (20 mM HEPES pH 7.5 ...
-
bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
-
bioRxiv - Biochemistry 2023Quote: ... we developed a scintillation proximity assay (SPA) using purified receptor and 3H-cholesterol (PerkinElmer). To capture M1-FLAG tagged receptor ...
-
bioRxiv - Cancer Biology 2021Quote: ... the samples were measured in AlphaScreen microplate reader (EnSpire Alpha 2390 Multilabel Reader, PerkinElmer).