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Citations for PerkinElmer :
1 - 50 of 332 citations for Recombinant Human Aldehyde Dehydrogenase 3 Family MemberA1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Microbiology 2023Quote: Apical wash fractions collected pre- and post-infection were used to detect Lactate dehydrogenase activity and IL-8 concentrations using human LDH and IL-8 Alphalisa Kits (Perkin Elmer). ELISA Kits for the detection of human TNFα ...
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bioRxiv - Microbiology 2020Quote: Infected mice were anesthetized using isofluorane and 1 μl of decyl aldehyde was instilled into each nostril before placement in a BSL-2 chamber within the IVIS instrument (IVIS Spectrum, Perkin Elmer). Isofluorane anesthesia was sustained during IVIS imaging ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Biochemistry 2023Quote: Endogenously tagged HiBiT cells were plated in 96-well plates (PerkinElmer) at a density of 0.5×106 cells/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Neuroscience 2021Quote: ... RNA probes were tagged with dinitrophenol-11-UTP (DNP, Perkin Elmer, Waltham, Massachusetts), fluorescein-12-UTP (FITC ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cancer Biology 2020Quote: H2B-mRFP tagged cells were imaged using an OperaTM High Content Screening instrument (PerkinElmer), equipped with a 20x air objective (LUCPLFLN ...
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Human IL-2 concentration was measured with the AlphaLISA human IL2 Kit (PerkinElmer) and data collected on an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Developmental Biology 2019Quote: ... and cyanine 3 (PerkinElmer), respectively ...
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bioRxiv - Developmental Biology 2021Quote: ... cyanine 3 (PerkinElmer #NEL744E001KT), and cyanine 5 (PerkinElmer #NEL745E001KT) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were tagged with GFP/luciferase to facilitate tracking via In Vivo Imaging Software (IVIS, Perkin Elmer)51 ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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SPNS1 is required for the transport of lysosphingolipids and lysoglycerophospholipids from lysosomesbioRxiv - Biochemistry 2022Quote: ... and lower phase (containing [3-3H]-sphingosine, [3-3H]-ceramides, [3-3H]-SM) were quantified by a scintillation counter (Perkin Elmer Tri-Carb Liquid Scintillation Analyzer).
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL γ32P-dATP (Perkin Elmer), and 5 μL H2O for 1 hour with shaking (1250 rpm ...
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bioRxiv - Microbiology 2021Quote: ... 3 pM GTP α-32P (PerkinElmer), 10 Units of VCE and incubated 30 min at 37°C.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 0.5 ml Optiphase HISAFE 3 (PerkinElmer) was incubated with the filter overnight before liquid scintillation counting with MicroBeta JET (PerkinElmer) ...
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bioRxiv - Genomics 2022Quote: ... and TSA Plus Cyanine 3 (PerkinElmer). After the last washing step ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Systems Biology 2023Quote: ... Finally 100 μl sample was added to scint vials containing 3 mls scintillant (OptiPhase HiSafe 3, Perkin Elmer). β-radioactivity was measured with Tri-Carb 4910TR liquid scintillation counter.
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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bioRxiv - Immunology 2019Quote: ... cyanine 3 and cyanine 5 (Perkin Elmer). The ACD 3-plex negative control probe was run in parallel on separate sections in each experiment to assess the background level and set the acquisition parameter ...
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bioRxiv - Systems Biology 2020Quote: ... Cy3-TSA (3 min; Perkin Elmer NEL744001KT), Cy5-TSA (7 min ...
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bioRxiv - Microbiology 2021Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Immunology 2021Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μL 35S-labeled methionine (PerkinElmer NEG709A500UC), 2.5 μL recombinant protein (~450ng ...
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bioRxiv - Microbiology 2022Quote: ... in XIC-3 animal isolation chamber (PerkinElmer) that provided biological isolation of anesthetized mice or individual organs during the imaging procedure ...
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bioRxiv - Immunology 2023Quote: ... using anti-human IFN-γ capture antibody (Pharmingen, 551221), biotinylated anti-human IFN-γ detection antibody (Pharmingen, 554550) and DELFIA Europium-labelled streptavidin (PerkinElmer), for fluorescent detection and quantification.