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Citations for PerkinElmer :
1 - 50 of 712 citations for Recombinant HIV 1 gp120 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Virus stocks were analyzed for HIV-1 p24 antigen by the AlphaLISA HIV p24 kit (PerkinElmer). Virus stocks contained approximate 200 ng/ml of HIV p24 protein.
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Molecular Biology 2019Quote: ... The DuPont HIV-1 p24 Core Profile ELISA kit (Perkin Elmer, Waltham ...
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bioRxiv - Immunology 2023Quote: ... Pelleted particles were resuspended in D10 medium and the concentration of HIV p24 was quantitated by using an HIV-1 p24 ELISA Kit (Perkin Elmer, Boston, MA, USA) [44 ...
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bioRxiv - Immunology 2021Quote: ... Viral titers were quantified by HIV-1 p24 AlphaLISA assay (PerkinElmer; AL291F) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... Viral stocks were titrated using HIV-1 P24 ELISA assay (#NEK05001KT, Perkin Elmer) and the concentration was 200 ng/ml.
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bioRxiv - Immunology 2019Quote: ... 14 and 21 for HIV-1 p24 antigen levels by ELISA (Perkin Elmer, Boston, MA).
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bioRxiv - Microbiology 2023Quote: ... 0.45 µm-filtered and used for HIV-1 CAp24 quantification by ELISA (released CAp24) (Perkin Elmer). Cell lysates were analyzed by western blotting.
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Microbiology 2021Quote: ... Gag p24 antigen concentration was measured with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Levels of HIV-1 gag p24 in culture supernatants were measured by ELISA as instructed by manufacturer (PerkinElmer).
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bioRxiv - Microbiology 2022Quote: ... by measuring the Gag p24 antigen concentration with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2021Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer) and/or by determining their infection titers on target cells.
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bioRxiv - Microbiology 2022Quote: ... Viral particles were titrated using an HIV-1 p24Gag Alpha-Lisa kit and an Envision plate reader (Perkin Elmer). VSV-G pseudotyped HIV-1 (NL4.3 ...
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bioRxiv - Microbiology 2022Quote: ... Infection was quantified after 24 h by FACS (for HIV-1-mCherry) or by adding 50 μl/well britelite plus reagent (PerkinElmer) and measurement on a Pherastar FS (BMG ...
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bioRxiv - Microbiology 2023Quote: ... Replication-competent HIV-1 stocks were normalized by p24Gag levels determined by ELISA (PerkinElmer Life Sciences, Inc., Boston, MA, USA).
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Genetics 2022Quote: ... The physical titer of vector preparations was measured using the HIV-1 Gag p24 antigen immunocapture assay kit (PerkinElmer, Waltham, MA, USA) and expressed as p24 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and Eu3+-tagged streptavidin (1244-360, PerkinElmer) diluted 1:1,000 in TBS/Tw containing 25 μM EDTA were subsequently added to the wells and incubated at RT for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Microbiology 2021Quote: ... The level of HIV-1 replication was determined by measuring p24 antigen in the culture supernatant using a kit (Perkin Elmer Inc, Shelton, CT, USA).
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bioRxiv - Microbiology 2021Quote: Concentration of HIV in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA).
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Biochemistry 2023Quote: Endogenously tagged HiBiT cells were plated in 96-well plates (PerkinElmer) at a density of 0.5×106 cells/mL ...
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bioRxiv - Microbiology 2022Quote: ... Concentration of HIV p24 antigen in each stock was determined by a p24 antigen capture kit (PerkinElmer, Waltham, MA, USA) and the concentration of total virus proteins were determined by a BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA probes were tagged with dinitrophenol-11-UTP (DNP, Perkin Elmer, Waltham, Massachusetts), fluorescein-12-UTP (FITC ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Cancer Biology 2020Quote: H2B-mRFP tagged cells were imaged using an OperaTM High Content Screening instrument (PerkinElmer), equipped with a 20x air objective (LUCPLFLN ...
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were tagged with GFP/luciferase to facilitate tracking via In Vivo Imaging Software (IVIS, Perkin Elmer)51 ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Cancer Biology 2020Quote: ... Signals from luciferase-tagged cells were monitored at day 0 after injection and weekly by in vivo imaging using IVIS Lumina II (Perkin Elmer) for 5 weeks ...
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bioRxiv - Molecular Biology 2022Quote: ... Then for the 6xHIS-tagged HAT module a 10 µL mixture of 5 µg/mL AlphaLISA Nickel chelate acceptors beads (PerkinElmer #AL108) and 10 µg/mL AlphaScreen donor beads (PerkinElmer #6760002) ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...