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Citations for PerkinElmer :
1 - 50 of 250 citations for Recombinant Canine OSM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... supplemented with canine pancreatic rough microsomes (tRNA probes, College Station, TX) and EasyTag 35S methionine (PerkinElmer, Waltham, MA). In vitro translation reactions were then diluted 1:4 into 1X SDS PAGE sample buffer and separated using a 12% SDS PAGE gel ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with canine rough microsomes (tRNA probes, College Station, TX) and EasyTag 35 S-labeled methionine (PerkinElmer, Waltham, MA) for one hour at 30 °C ...
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bioRxiv - Biochemistry 2023Quote: ... was mixed in 20 µl of the cytoplasmic buffer with 2- to 10-fold excess of recombinant Sm proteins and each sample was supplemented with 2 µCi of 3H-labeled SAM (PerkinElmer) for overnight methylation at 32 °C ...
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bioRxiv - Bioengineering 2022Quote: Saturation assays for binding of K1K1H6 to recombinant MET-Fc protein were performed in 384-well microtiter plates (OptiPlate™-384, PerkinElmer© ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... or recombinant MBP-β-tubulin were incubated with 6 μM radioactively labeled [3H] AdoMet (Perkin Elmer #NET155V001MC) in 1X HMT buffer containing 5 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant Ism1 or Igf1 with the addition of 0.5 μCi [35S]-methionine (#NEG009L005MC, PerkinElmer, Waltham, MA, USA) for 48h ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... assays were carried out by incubating 500ng of methyltransferases Rv2067c or DOT1L with 1–2 μg of recombinant mammalian histones or MtHU and 80μCi 3H-SAM (PerkinElmer) in a buffer containing 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: [125 I]-Bolton-Hunter labeled Transforming Growth Factor- β1 (Human, Recombinant) (catalog NEX267) was obtained from Perkin Elmer (Waltham, MA) This product was however discontinued in 2019 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Pathology 2024Quote: ... Recombinant human BMP4-induced pSMAD1 signalling was measured by AlphaLISA SureFire Ultra p-SMAD1 (Ser463/465) assay kit (Perkin Elmer). To perform the assay ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: Tritiated cGAMP was synthesized via a bio-catalytic reaction in which recombinant cGAMP synthase preactivated with herring DNA was incubated with [3H]-ATP (Perkin Elmer) and [3H]-GTP (Perkin Elmer ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
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bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by enhanced chemiluminescence (PerkinElmer, cat# NEL105001EA). Figures show representative images of 3-5 biological replicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... gravity-flow protein purification with Lipidex 1000 (Perkin Elmer) chromatography was conducted ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with Western Lightning Plus substrate (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: Proteins were directly radioiodinated with [125I]NaI (Perkin Elmer) using Pierce iodination reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer) and Anti-FLAG Alpha Donor Beads (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... Proteins were subsequently blotted onto PROTRAN Pure nitrocellulose membrane (PerkinElmer) using transfer buffer (25mM TRIS ...
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bioRxiv - Microbiology 2021Quote: ... proteins were detected with WesternLightning ECL Reagent (Perkin Elmer, Germany) and exposure of the membrane to CL-XPosure film (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were revealed using Lightning® Plus ECL (Perkin Elmer) and the bands were analysed by densitometry using ImageJ (NIH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein signal was detected using enhanced chemiluminescence (ECL) reagent (PerkinElmer). The antibodies used were obtained from commercial sources ...
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... and 1 µL Express 35S protein labelling mix (Perkin Elmer) in the presence of 0.1% (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... And proteins were visualized using enhanced chemiluminescence detection (NEL103E001EA, PerkinElmer) by AI600 (GE Healthcare).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.08 μl of Protein A-coated acceptor beads (PerkinElmer) in AlphaScreen buffer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 0.08 μl of Protein A-coated acceptor beads (PerkinElmer) in AlphaScreen buffer ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 of EasyTagTM EXPRESS 35S Protein Labeling Mix (Perkin Elmer) (5.5 μCi ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using Western Lightning ECL Plus (Perkin Elmer) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µg/mL AlphaLISA protein-A acceptors beads (PerkinElmer #AL101) and 10 µg/mL donor beads were added ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... 20 µCi/mL EasyTag Express 35S Protein Labeling Mix (PerkinElmer) was added to cultures and temperature was reduced to 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with Western Lightning ECL Pro substrate (PerkinElmer).
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bioRxiv - Microbiology 2020Quote: ... Separated proteins were then transferred to a PVDF membrane (Perkin Elmer) for 90 min with a semidry blotter (Peqlab ...
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bioRxiv - Neuroscience 2020Quote: ... Protein signals were developed by using ECL kit (NEL103001EA, Perkin Elmer). Quantification of interested protein bands were conducted by using NIH Image J software ...
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bioRxiv - Cell Biology 2020Quote: ... and supplemented with EasyTag 35S Protein Labeling Mix (Perkin Elmer, NEG772007MC), glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... protein expression levels were detected with Western Lightening Ultra (Perkin Elmer) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...