Labshake search
Citations for PerkinElmer :
1 - 50 of 617 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Semi-quantitative RT-PCR was carried out in the presence of [α-32P] dCTP (Perkin Elmer) with primer sequences provided in Supplementary Table 1 ...
-
bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:2000 ...
-
bioRxiv - Neuroscience 2021Quote: ... incubated with 3% of H2O2 for 30 minutes at RT and blocked 1 hour RT with NEN blocking buffer prepared according to the manufacturer’s protocol (FP1012 PerkinElmer). AntiDIG antibody conjugated to HRP (1:1500 ...
-
bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250, NEL749A001KT PerkinElmer). After several washes ...
-
bioRxiv - Neuroscience 2021Quote: ... during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100, NEL700A001KT PerkinElmer). After several washes ...
-
bioRxiv - Cell Biology 2023Quote: ... The PCR products were sequenced with the BigDye terminator kit (Perkin Elmer Applied Biosystems) using ABI3100 capillary sequencer (Perkin Elmer Applied Biosystems) ...
-
bioRxiv - Microbiology 2022Quote: ... coated (10%, 1.5h, RT) Cell Carrier-96 well plate (Perkin Elmer). After centrifugation (5 min ...
-
bioRxiv - Microbiology 2023Quote: ... coated (10%, 1.5h, RT) Cell Carrier-96 well plate (Perkin Elmer). After centrifugation (5 min ...
-
bioRxiv - Developmental Biology 2020Quote: ... PCR products were cleaned with the Biomek FX robot using the chemagic SEQ Pure20 Kit (PerkinElmer, CMG-458). Clean PCR amplicons from the same DNA sample were pooled to generate 137 libraries that were sequenced by Illumina MiSeq v3 ...
-
bioRxiv - Cancer Biology 2020Quote: ... in PBS for 1h at RT and with TNB blocking buffer (PerkinElmer) for 30 min for EF5 staining ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 5 minutes at room temperature (RT) in accordance with manufacturer’s instructions (PerkinElmer). After 3 times wash by TNT ...
-
bioRxiv - Neuroscience 2024Quote: ... incubated in TNB (Blocking Reagent [Perkin Elmer; 0.05% in TN]; 30 min, RT), incubated with anti-DIG-POD antibody (Roche ...
-
bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
-
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... washed and finally incubated for 8 min at RT with Tyramide-Cy5 (Perkin Elmer) dil ...
-
bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
-
bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
-
bioRxiv - Neuroscience 2021Quote: ... for 1h at RT with following blocking with TSA blocking reagent (Perkin Elmer, Waltham, MA, USA, FP1020) as 0.5% solution in PBS pH 7.4 for another 1h at RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... the samples were incubated at RT for 30 min and measured with EnVision multi-plate reader (PerkinElmer).
-
bioRxiv - Immunology 2019Quote: ... and scanned at RT using 20X objective on the Vectra® 3 automated quantitative pathology imaging system (PerkinElmer) (Fig ...
-
An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 30 min at RT with streptavidin-HRP (Perkin Elmer) dil ...
-
bioRxiv - Microbiology 2020Quote: ... for 5 min at RT and measured at 450 nm by the microplate reader Victor Nivo (Perkin Elmer).
-
bioRxiv - Microbiology 2021Quote: Culture supernatants were assayed for RT activity by surveying for 3H-thymidine incorporation (3H-TTP; Perkin Elmer #NET221A001MC). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... Generated AFC signal is detected after 15 min at RT using Ex/Em = 360/460 nM (Envision, PerkinElmer). Final assay concentrations ...
-
bioRxiv - Immunology 2022Quote: ... PCR products are visualized on Caliper LabChip (PerkinElmer).
-
bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
-
bioRxiv - Biophysics 2019Quote: ... The bead slurry was incubated at RT for 30 minutes before reading on an EnVision plate reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
-
bioRxiv - Genomics 2022Quote: ... The slides were blocked in TNB buffer at RT for 30 minutes and incubated with antifluorescein-HRP Conjugate (PerkinElmer) in TNB (diluted 1:1000 ...
-
bioRxiv - Immunology 2020Quote: ... Agencourt AMPure XP beads and PCR amplified using KAPA hot start High-Fidelity 2X PCR Master Mix and NextFlex index primers (Bioo Scientific, PerkinElmer) for 12 cycle by following thermocycler cycles ...
-
bioRxiv - Biophysics 2021Quote: ... Plates were incubated at RT for 40 min and read according to the protocol using an EnVision multilabel reader (PerkinElmer) with the emission window ratio of 665 nm over 620 nm ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were incubated in the dark for 2 h at RT before measuring fluorescence using the EnVision Multimode Plate Reader (PerkinElmer) with excitation and emission wavelengths of FITC 485 / FITC 535 ...
-
bioRxiv - Microbiology 2022Quote: ... The plate was then incubated for 1 h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (PerkinElmer). Negative control with binding buffer or only with one of the complexes were used to control the assay quality ...
-
bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and incubated with TSA Plus Fluorescein (PerkinElmer) according to the manual at RT for 3-15 minutes (3-5 min in H ...
-
bioRxiv - Genomics 2022Quote: ... the slides were washed three times in 1x TNT at RT for 5 minutes each and the amplification steps were repeated using anti-DNP-HRP Conjugate (PerkinElmer) and TSA Plus Cyanine 3 (PerkinElmer) ...
-
bioRxiv - Biophysics 2023Quote: ... The absorption and emission spectra of the purified protein were recorded at RT (Lambda365, Perkin Elmer and FLS980, Edinburgh Instruments)
-
bioRxiv - Microbiology 2021Quote: ... The plate was then incubated for 1h at RT in the dark before the AlphaLISA signal was detected using an EnSpire Multimode Plate Reader (Perkin Elmer). Negative control with binding buffer was used to control the assay quality ...
-
bioRxiv - Neuroscience 2023Quote: ... The slides were thawed and fixed for two min with 0.1% paraformaldehyde in PBS at RT and then incubated in 50 pM I125-OVTA (2200 Ci/mmol; PerkinElmer; Boston, MA), a selective ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 μl of Universal PCR Master Mix (PerkinElmer Applied Biosystems) and 7.7 μl of nuclease-free water ...
-
bioRxiv - Bioengineering 2022Quote: ... The reactions were terminated with 1M H3PO4 after 20 minutes incubation at RT and optical density (OD) was measured by a spectrophotometer at 450nm (PerkinElmer EnVision 2105).
-
bioRxiv - Bioengineering 2022Quote: ... The reactions were terminated with 1M H3PO4 after 20minutes incubation at RT and optical density (OD) was measured by a spectrophotometer at 450nm (PerkinElmer EnVision 2105).
-
bioRxiv - Bioengineering 2019Quote: ... The PCR products were validated using a LabChip GX II (Perkin Elmer) and sequenced using PlateSeq PCR Kits (Eurofins ...
-
Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... OPAL kit (PerkinElmer) fluorophores were used and prepared according to the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR products were used as template to incorporate radioactive 32P-dCTP (Perkin Elmer) using the High Prime kit (Roche ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
-
bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The PCR products were then transferred to GeneScreen Plus Hybridization Transfer Membrane (Perkin Elmer) and detected by hybridization using a non-radioactive method as described in Tomé et al.30.