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Citations for PerkinElmer :
1 - 33 of 33 citations for Phospholipase A2 Activating Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: hTert-RPE1 mTurquoise-H2B mRuby-PCNA Cyclin A2-Venus cells were reverse transfected in 384w PhenoPlates (PerkinElmer) with non-targeting control (NTC ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Biochemistry 2022Quote: ... 25 nM biotinylated and hexahistidine-tagged linker peptide (Bn-His6) (PerkinElmer) was added to the compounds ...
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bioRxiv - Immunology 2021Quote: ... Peptide masses were initially predicted by ChemDraw Professional 15.0 (PerkinElmer, Massachusetts, USA) and compared to acquired masses after LC-MS measurements.
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bioRxiv - Microbiology 2021Quote: ... an equal volume of biotinylated SRC1 peptide/Europium-labeled streptavidin (Perkin Elmer) was added to biotynlated RORγ/APC-labeled strepavidin (Perkin Elmer) ...
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bioRxiv - Biochemistry 2020Quote: ... Peptide stocks in DMSO were spotted onto 384-well white OptiPlates (PerkinElmer Life Sciences) at concentration ranges of 1 nM to 100 M using a Labcyte Echo 555 Omics acoustic liquid handler (Labcyte ...
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bioRxiv - Bioengineering 2019Quote: ... Aliquots of the peptide were deposited on a “Golden Gate” diamond ATR (PerkinElmer, USA). PBS was used as a background spectrum ...
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bioRxiv - Neuroscience 2021Quote: ... Compound plates containing synthetic peptides in DMEM/BSA were placed in a MicroBeta LumiJet luminometer (PerkinElmer). After loading ...
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bioRxiv - Molecular Biology 2021Quote: Peptide or small molecules stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Reactions were performed with 50 nM ULight-Glycogen Synthase (Ser641/pSer657) peptide (Perkin Elmer #TRF0131-M), 15 μM ATP ...
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bioRxiv - Molecular Biology 2022Quote: Peptide-or small molecule-stimulated cAMP accumulation was measured by a LANCE Ultra cAMP kit (PerkinElmer). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... The amount of peptide remaining bound was measured using scintillation counting in Ultima Gold (Perkin Elmer). Experiments were performed a minimum of three times to generate an overall mean and standard deviation ...
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bioRxiv - Biochemistry 2022Quote: ... The structures of NTV and the substrate peptide were drawn in ChemDraw 20.0 (Perkin Elmer, USA) and optimized to generate 3D conformations using the LigPrep module in Maestro.
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bioRxiv - Biochemistry 2021Quote: ... Substrate peptides or nucleosomes were mixed in a solution containing 9µM tritiated S-Adenosyl Methionine (SAM, Perkin Elmer) cofactor ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: In vitro peptide-based Aur A assays were carried out using a Caliper LapChip EZ Reader platform (Perkin Elmer), which monitors real-time phosphorylation-induced changes in the mobility of a fluorescently labelled Kemptide peptide substrate (5’-FAM-LRRASLG-CONH2 ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... A LANCE Ultra TR-FRET kinase assay was used for measurements of enzyme activities that included an-Ulight labeled (Thr-1342) peptide substrate (catalog #TRF0130-M, PerkinElmer) and a Europium-labeled anti-phospho peptide antibody (catalog # TRF0218-M) ...
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bioRxiv - Biochemistry 2020Quote: ... was incubated with 5 μL of biotinylated peptides at 100 nM for 30 min at room temperature in the epigenetics assay buffer (PerkinElmer AL008 ...
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bioRxiv - Neuroscience 2022Quote: ... were challenged with synthetic peptides reconstituted in DMEM/BSA medium and luminescence was measured on a MicroBeta LumiJet luminometer (PerkinElmer). Luminescence was measured for 30 s at a wavelength of 469 nm ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Radioactive counts from the media that remain soluble after TCA precipitation (peptide fragments) and pellets from the cells (total non-degraded cell proteins) were measured in a TopCount radioactivity detector (Perkin Elmer). A “protein degradation index” was calculated as the ratio between the counts detected in the soluble media and the counts detected in the pellet cell fraction (82).
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2019Quote: ... Standard Fmoc-peptide synthesis was used to synthesize peptides on the amino-functionalized solid support by custom modified JANUS liquid handling stations (Perkin Elmer). The binding of antibody to each of the synthesized peptides was tested in a pepscan-based ELISA ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2021Quote: ... and the level of 32P-transfer to the SAMS peptide was determined using a Tri-Carb 4810TR liquid scintillation counter (Perkin Elmer).
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of 40 μM SFK peptide was added to each well and raw fluorescence units were measured immediately on an Envision fluorometer (Perkin Elmer) with an excitation wavelength of 344 nm and an emission wavelength of 405 nm in real time for the first 15 min at 15 s intervals ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... where reactions were incubated with 150 nM of the gel-filtration purified WT mouse Naa10 or Naa12 in a 30 µl reaction volume containing each 250 µM substrate peptide and radiolabeled [14C]acetyl-CoA (4 mCi/mmol; PerkinElmer Life Sciences) for 12 min (Naa12 ...
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bioRxiv - Cancer Biology 2023Quote: ... The phospho-peptide product was measured by time-resolved fluorescence resonance energy transfer (TR FRET) between the terbium of the antibody and fluorescein of the phospho-peptide using an EnVision plate reader (PerkinElmer, Waltham, MA) equipped to detect the TR-FRET signal (excitation 320 nm ...
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bioRxiv - Bioengineering 2022Quote: ... PEG-maleimide was reacted with 1 mol equivalence of calpain substrate peptide and 1 mol equivalence of L-cysteine-functionalized VivoTag S-750 (PerkinElmer, Boston, MA), followed by quenching with an excess of L-cysteine ...
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bioRxiv - Cell Biology 2020Quote: LH levels in the medium were measured by means of a time-resolved fluorometric immunoassay with rat LH assay kits (National Hormone and Peptide Program, NHPP) and the Delfia system (Perkin Elmer, Waltham, MA) as reported previously (Kawaminami et al. ...
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bioRxiv - Neuroscience 2023Quote: ... Reactions were initiated by adding 2.5 µM ATP at 27°C and were terminated after 60 min with 10 µL of 2.5X Stop Solution/Detection Mix containing 20mM EDTA and 4nM LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide antibody (Perkin Elmer, Waltham, MA). After incubation at 27°C for another 60 min ...