Labshake search
Citations for PerkinElmer :
1 - 50 of 97 citations for PD L1 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 3T3-L1 cells were seeded on CellCarrier Ultra 96-well plates (PerkinElmer). When cells reached confluency ...
-
bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
-
bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 384-well PhenoPlates (PerkinElmer #6057302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media supplemented with doxycycline (1 ug/mL) ...
-
bioRxiv - Bioengineering 2022Quote: ... HEK293-ACE2 cells were seeded in 384-well white plates (PerkinElmer, USA), allowed to attach overnight followed by addition of pseudoviral particles in the presence or absence of varying concentrations of GRFT ...
-
bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
-
bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
-
bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
-
bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
-
bioRxiv - Neuroscience 2020Quote: Larval stage 1 animals (L1) were imaged in a UltraView VoX spinning-disc confocal microscope (PerkinElmer Life and Analytical Sciences). Embryonic imaging was performed via dual-view inverted light sheet microscopy (diSPIM)64,65 ...
-
bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
-
bioRxiv - Systems Biology 2022Quote: T-REx HEK293 cells stably transfected with LRRK2 plasmids were seeded in 96-well PhenoPlates (PerkinElmer #6055302) manually coated with collagen I in T-REx base media ...
-
bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
-
bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
-
bioRxiv - Biophysics 2021Quote: ... HEK293 TauRD-P301S-CFP/YFP expressing biosensor cells were plated in poly-L-lysine coated 96-well plates (PerkinElmer) at a density of 20000 cells/well and subsequently transfected with pre-incubated mixtures of tau seeds/CAP1 ...
-
bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
-
bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
-
bioRxiv - Neuroscience 2019Quote: ... were transfected into the indicated cells (HEK293/17: 4×105/well; CHO: 2.5×104/well, BMVECs: 5×103/well) in 96-well plates (PerkinElmer, 6005680) in triplicate ...
-
bioRxiv - Biochemistry 2021Quote: ... microsomes prepared from HEK293 cells expressing RyR2 were incubated for 1 h at 25 °C with 5 nM [3H]ryanodine (PerkinElmer) in reaction media containing 0.17 M NaCl ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Binding experiments were performed on membranes prepared from wild-type and HA-MOP-transfected HEK293 cells using [3H]DAMGO (51.7 Ci/mmol) (PerkinElmer NET902250UC) as described previously52 ...
-
bioRxiv - Cell Biology 2020Quote: Two days after transfection the Hek293 cells were washed with PBS and seeded into a 96 well black-white iso plate (Perkin Elmer) in pbs containing 5 mM glucose and supplemented with 5 µM of Nanoglo (Promega #N1120 ...
-
bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
-
bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
-
bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
-
bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
-
bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
-
bioRxiv - Developmental Biology 2020Quote: ... mouse dissociated commissural neurons and mouse dorsal spinal cord explants were imaged using a spinning disk confocal system (Perkin Elmer) built on a Nikon Ti-U inverted microscope using a Nikon 40X objective (for nerve cords and neurons ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Bioengineering 2022Quote: ... OsteoSense 680EX (80 nmol/kg/mouse, PerkinElmer, NEV10020EX). After 48 hours ...
-
bioRxiv - Cancer Biology 2019Quote: ... 5 µl of anti-Mouse Acceptor beads (PerkinElmer, diluted 1:500 with 1x detection buffer ...
-
bioRxiv - Microbiology 2021Quote: ... and then living mouse and mouse organs were imaged and detected using the small animal in vivo imaging system (PerkinElmer, USA). The mouse organs were fixed with formalin ...
-
bioRxiv - Bioengineering 2022Quote: ... plates were incubated with anti-mouse IgG-Europium antibody (PerkinElmer) for 1 hour ...
-
bioRxiv - Bioengineering 2023Quote: ... and HRP-labeled goat anti-mouse (PerkinElmer Life Science, USA) staining ...
-
bioRxiv - Genetics 2023Quote: ... each mouse was gavaged with 1 µCi 59FeCl2 (Perkin Elmer) in 2.21 mM FeCl3 in 1 M ascorbic acid filter-sterilized ...
-
bioRxiv - Bioengineering 2020Quote: ... Whole-mouse luminescence data were analyzed using Living Image software (PerkinElmer). Figures were designed in Adobe Illustrator.
-
bioRxiv - Biochemistry 2024Quote: ... HRP labelled goat anti-mouse IgG (Perkin Elmer: NEF822001EA, 1:10,000), HRP labelled goat anti-rabbit IgG (Perkin Elmer ...
-
bioRxiv - Pathology 2022Quote: ... or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer, Inc). Nuclei were counterstained with DAPI.
-
bioRxiv - Cell Biology 2022Quote: ... 1:200 dilution of anti-mouse IgG donor bead (Perkin Elmer, AS104D) and a 1:200 dilution of anti-rabbit IgG acceptor bead (Perkin Elmer ...
-
bioRxiv - Neuroscience 2021Quote: ... Each mouse received 2 nmol of MMPSense 680 (150 μl; Perkin Elmer, France) or Cat K 680 FAST™ (100 μl ...
-
bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
-
bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
-
bioRxiv - Cancer Biology 2023Quote: ... The appearance of mouse metastasis was monitored by IVIS (IVIS Lumina III, Perkin Elmer) after injecting the animals with luciferine (150 mg/kg ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 min with horseradish peroxidase-conjugated secondary polymer (anti-mouse/anti-rabbit, Perkin Elmer), and 10 min with horseradish peroxidase-reactive OPAL fluorescent reagents (Perkin Elmer) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 minutes after intraperitoneal injection of Potassium D-Luciferin solution (Perkin Elmer, 3 mg/mouse). Luminescence values for all xenografts were quantified as the average radiance (photons/s/cm2/sr ...
-
bioRxiv - Cancer Biology 2022Quote: ... each mouse was given an intra-peritoneal (i.p.) injection of luciferin (PerkinElmer, Part Number #122799) at a dose of 150mg/kg body weight ...
-
bioRxiv - Molecular Biology 2021Quote: ... was captured on anti-mouse IgG (Fc specific) acceptor beads (Perkin Elmer, Cat nr. AL105C). Serial dilutions of antibodies and VHH-Fc (final concentration ranging between 100nM - 0,001 nM ...
-
Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... an anti-mouse IgG (1.5nM) labelled with fluorescence donor Eu-W1024 (Perkin Elmer product AD0076) and an anti-rabbit IgG (14.3nM labelled with acceptor (APC) ...