Labshake search
Citations for PerkinElmer :
1 - 50 of 604 citations for PCR Product Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were sequenced with the BigDye terminator kit (Perkin Elmer Applied Biosystems) using ABI3100 capillary sequencer (Perkin Elmer Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... PCR products are visualized on Caliper LabChip (PerkinElmer).
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were cleaned with the Biomek FX robot using the chemagic SEQ Pure20 Kit (PerkinElmer, CMG-458). Clean PCR amplicons from the same DNA sample were pooled to generate 137 libraries that were sequenced by Illumina MiSeq v3 ...
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bioRxiv - Bioengineering 2019Quote: ... The PCR products were validated using a LabChip GX II (Perkin Elmer) and sequenced using PlateSeq PCR Kits (Eurofins ...
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were prepared using 10 ng of purified PCR product with a NEXTFLEX® Rapid XP DNA-Seq Kit (PerkinElmer) barcoded using NEXTFLEX® UDI Barcodes (PerkinElmer) ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were used as template to incorporate radioactive 32P-dCTP (Perkin Elmer) using the High Prime kit (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR products were checked by capillary electrophoresis on a Caliper LabChip GX analyzer (PerkinElmer). The products of the second PCR were purified with a NucleoSpin 96 PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were then transferred to GeneScreen Plus Hybridization Transfer Membrane (Perkin Elmer) and detected by hybridization using a non-radioactive method as described in Tomé et al.30.
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bioRxiv - Plant Biology 2023Quote: ... The size and abundance of the resultant PCR products were then analyzed and quantified by LabChip GX bioanalyzer (PerkinElmer). The expression analyses represent two to three biological replicates ...
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bioRxiv - Neuroscience 2023Quote: ... probes were fluorescently labeled with Opal Dyes (Opal 520 product no. FP1487001KT, Opal 570 product no. FP1488001KT, Opal 690 product no. FP1497001KT; Perkin Elmer). Autofluorescence from lipofuscin was quenched using the reagent TrueBlack (Biotium ...
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bioRxiv - Neuroscience 2021Quote: ... Poly-adenylated RNA was isolated from each sample using the NEXTflex PolyA Beads purification kit (Perkin Elmer). Strand-specific libraries with unique barcodes were prepared using the NEXTFLEX Rapid Directional RNA-Seq kit 2.0 according to manufacturer’s instructions (Perkin Elmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... Probes were prepared by random priming of corresponding PCR products (5’ and GFP templates) in the presence of [αP32]CTP (PerkinElmer BLU513H100UC). Unincorporated nucleotides were removed using a Sephadex G-50 column (GE Healthcare 28-9034-08) ...
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bioRxiv - Molecular Biology 2024Quote: ... 120 ng PCR product was mixed with 7.5 mM ribonucleotide solution (ATP, GTP, UTP, CTP) and 133 nM α-32P-ATP (Perkin Elmer) as a tracer ...
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bioRxiv - Immunology 2022Quote: ... The PCR products were analyzed by heteroduplex mobility assay using microfluidic capillary electrophoresis system caliper LabChip GX (PerkinElmer, Villebon-sur-Yvette, France). The homozygous Il34-/- specimen were identified from Il34+/+ littermate as previously described (13).
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bioRxiv - Evolutionary Biology 2020Quote: ... were added to the PCR product and sequencing was conducted using the automated DNA Sequencer (ABI 3130xl Genetic Analyzer; Perkin Elmer/Applied Biosystems). Using the software Genemapper ver ...
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bioRxiv - Pathology 2023Quote: ... Supernatant was discarded and DNA was extracted using the QuicPickTM SML Plant DNA purification kit according to the supplier’s instructions (Bio-Nobile, Turku, Finland) in an automated system (Caliper Zephyr, PerkinElmer). DNA was eluted from magnetic beads using 30 µL of elution buffer ...
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bioRxiv - Genetics 2022Quote: ... Amplified products were quantified with Quant-iT(tm) PicoGreen dsDNA Assay Kit on a VICTOR Multilabel Plate Reader (PerkinElmer) and pooled in equimolar ratios ...
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bioRxiv - Microbiology 2020Quote: ... Stool samples underwent DNA extraction using chemical and mechanical lysis with magnetic bead-based purification via the Chemagic MSM I with the Chemagic DNA Blood Kit-96 from Perkin Elmer. Prior to extraction on the MSM-I ...
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bioRxiv - Evolutionary Biology 2020Quote: ... gravity-flow protein purification with Lipidex 1000 (Perkin Elmer) chromatography was conducted ...
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bioRxiv - Plant Biology 2020Quote: ... Final library products were assessed for quality on LabChip GX using the DNA High Sensitivity Reagent Kit (Perkin Elmer, MA, USA), diluted to 2 nM concentration and pooled ...
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bioRxiv - Neuroscience 2023Quote: The BACE cleavage product βCTF was detected by AlphaLISA (Perkin Elmer) Amyloid Beta kit (Cat # AL275C ...
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bioRxiv - Microbiology 2021Quote: ... and cleaned using 0.8× Ampure XP beads purification on a SciClone IQ (Perkin Elmer). Sequencing libraries were generated using two-fifths volumes of Nextera XT on ds-cDNA with 18 cycles of PCR amplification ...
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bioRxiv - Molecular Biology 2021Quote: HDL-sRNA libraries were generated using the NEXTFlex Small RNA-Seq Kit v3 with 1:4 adapter dilution and 22 PCR cycles (PerkinElmer). Following PCR amplification ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified product was sequenced on ABI 3730 DNA analyzer (PerkinElmer Biosystems, USA).
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bioRxiv - Immunology 2021Quote: ... Products of the second-step PCR reactions were purified and analysed with Caliper Labchip for DNA samples (HT DNA High Sensitivity LabChip Kit; Perkin Elmer) prior to sequencing with the Miseq or HiSeq2500 instrument for the library representation (Illumina) ...
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bioRxiv - Molecular Biology 2022Quote: ... adapter ligation and PCR were performed using the NEXTFLEX® Rapid DNA-Seq Kit 2.0 for Illumina® Platforms (PerkinElmer, UK) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The product 4-methylumbelliferon (MU) was quantified using a fluorimeter (Perkin Elmer LS55, USA). Protein concentration was determined by Bradford assay (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... Amplification products were analyzed for a size shift using a LabChip GX (Perkin Elmer). Selected samples with deletion sized products were purified using ExcelaPure 96-Well UF PCR Purification Kit (Edge Bio ...
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Development of a small molecule that corrects misfolding and increases secretion of Z α1-antitrypsinbioRxiv - Pharmacology and Toxicology 2020Quote: ... an anti-mouse IgG (1.5nM) labelled with fluorescence donor Eu-W1024 (Perkin Elmer product AD0076) and an anti-rabbit IgG (14.3nM labelled with acceptor (APC) ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using random hexamer primers as described by the manufacturer (GeneAmp RNA PCR Kit from Perkin Elmer Cetus, Norwalk, CT). PCR-mastermix containing the specific primers and Taq DNA polymerase (HotStar Taq ...
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bioRxiv - Biochemistry 2023Quote: ... The luminescence of the product was measured using an EnSpire 2300 Multilabel plate reader (Perkin Elmer). A linear regression fit of the SAH calibration curve was determined and served to interpolate SAH produced from the reactions ...
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bioRxiv - Biochemistry 2023Quote: ... The luminescence of the product was measured using an EnSpire 2300 Multilabel plate reader (Perkin Elmer). All assays were performed with three replicates.
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bioRxiv - Microbiology 2023Quote: ... with an additional purification step based on the use of silica-coated magnetic beads (GMG-252-A-100mL – PerkinElmer), as follows ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All assay plates used were OptiPlate-384 white well microplates (product number: 6007290, PerkinElmer LAS Ltd, UK) unless otherwise stated ...
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bioRxiv - Molecular Biology 2023Quote: ... The non-volatile products were measured in a Tri-Carb 2810 TR Liquid Scintillation Counter (PerkinElmer; Waltham, MA) after 48 h ...
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bioRxiv - Immunology 2020Quote: ... Agencourt AMPure XP beads and PCR amplified using KAPA hot start High-Fidelity 2X PCR Master Mix and NextFlex index primers (Bioo Scientific, PerkinElmer) for 12 cycle by following thermocycler cycles ...
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bioRxiv - Genomics 2021Quote: ... The total radioactivity of the reaction products was measured using a liquid scintillation counter (Tri-Carb 2910TR, Perkin Elmer). The kinetic constant was calculated by a nonlinear regression fit to the Michaelis-Menten equation using OriginPro® 8.6 (OriginLab Co. ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction products were transferred into Streptavidin-coated FlashPlates for scintillation counting using a TopCount instrument (PerkinElmer, Massachusetts, USA). Reaction mixtures were prepared in triplicate ...
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bioRxiv - Immunology 2021Quote: ... The 3H-labeled products were isolated using DEAE-Sephadex A-50 columns and quantitated by liquid scintillation counting (PerkinElmer). Non-isotopic methylated RNA fragments were prepared using cold SAM (Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... In vitro uptake or in vivo assays for the biodistribution of the reporter exosomes and MVs were imaged with IVIS Lumina or IVIS Spectrum systems (Xenogen product line of PerkinElmer). For in vitro assays ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 μl of Universal PCR Master Mix (PerkinElmer Applied Biosystems) and 7.7 μl of nuclease-free water ...
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bioRxiv - Cancer Biology 2024Quote: ... PrestoBlue: 590 nm) of the metabolic product generated was quantified in an Enspire multimode plate reader (PerkinElmer Sverige AB, Upplands Väsby, Sweden).
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bioRxiv - Physiology 2022Quote: ... The assay was a mobility shift assay based on charge differences before and after electrophoretic separation of product from fluorescently labeled substrate read using a Caliper EZ Reader (Perkin Elmer, Boston, MA). All reactions took place in the presence of excess nicotinamide adenine dinucleotide and the known SIRT1 inhibitor suramin was used as a control.
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Tumour Extracellular Vesicles Induce Neutrophil Extracellular Traps To Promote Lymph Node MetastasisbioRxiv - Cancer Biology 2023Quote: ... OPAL kit (PerkinElmer) fluorophores were used and prepared according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...
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bioRxiv - Genetics 2023
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bioRxiv - Cell Biology 2019Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols (Wang et al. ...
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bioRxiv - Physiology 2020Quote: ... Fluorescein Evaluation Kit System (PerkinElmer) were used according to manufactures’ protocols(Wang et al. ...
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bioRxiv - Biochemistry 2022Quote: ... (PerkinElmer AlphaScreen Histidine Detection Kit) were added under low light conditions ...