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Citations for PerkinElmer :
1 - 50 of 265 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and subsequently counted via Hoechst nuclear staining using Harmony software (PerkinElmer). A minimum of four fields were acquired per well using a 10x air objective in a confocal mode ...
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bioRxiv - Immunology 2024Quote: ... samples were stained with DAPI for nuclear counterstaining (PerkinElmer, MA, USA) and mounted in EverBrite Mounting Medium (Biotium).
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bioRxiv - Molecular Biology 2021Quote: ... nuclear run-on reactions were performed with 25μM biotin-11-UTP (PerkinElmer, NEL543001EA), 25μM biotin-11-CTP (PerkinElmer ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear run-on reactions were conducted with 25μM biotin-11-UTP (PerkinElmer, NEL543001EA), 25μM biotin-11-CTP (PerkinElmer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Hoechst-stained nuclear regions of interest were segmented using Harmony software (Perkin Elmer) and cell viability was expressed in each plate as the relative number of cellular events in sample wells relative to the average of NEG wells ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclear RELA was measured in all non-border cells using Harmony software (PerkinElmer). In R ...
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bioRxiv - Synthetic Biology 2021Quote: ... Cells were then segmented based upon the nuclear dye using the Harmony software (PerkinElmer). Propidium iodide intensity levels were calculated and cells were classified as ‘dead’ if their intensity was above the established threshold.
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bioRxiv - Cancer Biology 2020Quote: ... nuclear intensity was measured using ImageJ or the Operetta CLS High-Content Analysis System (Perkin Elmer). Number of cells or fields analysed were indicated
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bioRxiv - Cell Biology 2022Quote: ... Nuclear area and fluorescence intensity of Sytox Orange was analyzed by segmentation with Harmony software (PerkinElmer). Nuclei that were touching the image border were excluded from further analysis ...
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bioRxiv - Microbiology 2023Quote: Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Agonist-induced translocation from ER-peri-nuclear foci was imaged and quantified using EnVision Reader (PerkinElmer, Waltham, MA). Cells were fixed after exposure to compound for 1 hour and stained for with antibodies against STING ...
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bioRxiv - Genomics 2023Quote: ... on a Chemagic 360 automated nucleic extraction instrument (Perkin Elmer) following manufacturer’s protocols for DNA isolation from whole blood.
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bioRxiv - Genomics 2021Quote: ... Aliquots of 10E6 nuclei were subjected to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) and PRO-seq libraries were constructed in duplicate as described (31) ...
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bioRxiv - Genomics 2022Quote: ... PRO-seq libraries for all replicates were then prepared simultaneously by subjecting one aliquot of 1e7 nuclei/sample to 3-minute nuclear run-on reactions in the presence of Biotin-11-CTP (PerkinElmer) following our previously detailed protocol (24) ...
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bioRxiv - Cancer Biology 2021Quote: ... Contiguous areas were analyzed for presence or absence of NOTCH1 or nuclear NICD1 staining and their length along the section measured using Volocity 6 software (Perkin Elmer). For morphological analysis of NICD1 and positive negative areas (Extended data Fig.1 f-h) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were counterstained with DAPI nuclear counterstain and imaged using the Vectra Polaris multispectral imaging system (Perkin Elmer, Life Sciences, Zaventem, Belgium) and analysed using QuPath ...
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bioRxiv - Evolutionary Biology 2023Quote: ... DNA was extracted using the Chemagic360 DNA tissue extraction kit (PerkinElmer, CMG-723) on a Chemagic360 nucleic acid extractor (PerkinElmer ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Proteins were labelled for 5 mins (for soluble proteins) or 15 mins (for membrane proteins) with 30 µCi of Express Protein Labelling Mix (Perkin Elmer) per sample at their restrictive temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... blinded data extraction for mean-intensity and percent-area was conducted using inForm software from PerkinElmer. Normalization and Chi-square statistical analysis were conducted using Matlab.
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bioRxiv - Cell Biology 2023Quote: ... EXPRE35S35S Protein Labelling Mix (PerkinElmer); 150 μCi/10 plate] for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... An aliquot of the supernatant was processed for total nucleic-acid extraction using Chemagic 360 (Perkin Elmer). Nucleic acid preparations from wastewater samples are known to contain PCR inhibitors that interfere with their accurate quantification using PCR-based methods ...
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bioRxiv - Genetics 2024Quote: Genomic DNA of blood donors was extracted on a Chemagic MSM I extraction robot (Chemagen, Perkin Elmer). High-throughput genotyping of 46 blood group antigens of 35,954 donor samples collected in the years 2015 to 2021 was performed by MALDI-TOF MS as described previously [16,17] ...
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bioRxiv - Biochemistry 2019Quote: ... EasyTagTM EXPRESS35S Protein Labeling Mix (PerkinElmer) (1 μl/25-μl reaction ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Total nucleic acid was extracted from FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer). Percent tumor cellularity as a ratio of tumor to normal nuclei was verified against pathologist-derived assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were detected by ECL (Perkin Elmer). Antibodies used are listed in the key Resource Table.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred to PVDF membranes (PerkinElmer) and blocked in 5% nonfat milk in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... The DNA extraction from K2-EDTA-blood tubes was performed with chemagic 360 instrument and the CMG-704 kit (PerkinElmer), which uses magnetic bead technology ...
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bioRxiv - Physiology 2020Quote: ... Protein bands were detected using Western Lightning (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... [35S]-EasyTag™ Express Protein Labeling Mix (PerkinElmer) was added and the cells were incubated overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 µCi/ml and at given time points ...
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bioRxiv - Molecular Biology 2023Quote: ... EasyTag™ Express35S Protein Labeling-Mix (Perkin Elmer) was then added to a final count of 50 μCi/ml and at given time points ...
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bioRxiv - Cancer Biology 2022Quote: ... Solid tumor total nucleic acid was extracted from these tumor regions on adjacent FFPE tissue sections using Chemagic 360 sample-specific extraction kits (Perkin Elmer) and digested by proteinase K ...
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bioRxiv - Biochemistry 2020Quote: ... The purity of the proteins was assessed by SDS-PAGE and the protein concentration was determined using a spectrometer (PerkinElmer lambda40) with an extinction coefficient of 6990 M−1cm−1.
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bioRxiv - Microbiology 2020Quote: ... The precipitates proteins were resolved by SDS-PAGE and [32P]-labeled proteins was visualized using a Super Resolution screen (Perkin Elmer and quantified using a Cyclone Plus Storage Phosphor System (Perkin Elmer) ...
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bioRxiv - Cell Biology 2019Quote: ... protein bands were visualized with enhanced chemiluminescent reagent (PerkinElmer) and exposed to X-ray films (Carestream ...
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bioRxiv - Immunology 2021Quote: ... proteins were detected using Western Lightning Plus-ECL (PerkinElmer) and the iBright FL1000 Imaging System (Thermo Fisher ...
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bioRxiv - Biochemistry 2021Quote: SPA PVT Protein A beads (500μl/sample, Perkin Elmer) were incubated for 2h with anti-GST antibodies (1:100) ...
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bioRxiv - Biochemistry 2020Quote: ... and EasyTag EXPRESS [35S] Protein Labeling Mix (Perkin Elmer) for 30 min in a tissue culture incubator ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were visualized by enhanced chemiluminescence (PerkinElmer, cat# NEL105001EA). Figures show representative images of 3-5 biological replicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... gravity-flow protein purification with Lipidex 1000 (Perkin Elmer) chromatography was conducted ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were visualized with Western Lightning Plus substrate (PerkinElmer).
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bioRxiv - Bioengineering 2023Quote: Proteins were directly radioiodinated with [125I]NaI (Perkin Elmer) using Pierce iodination reagent (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... After diluting Alpha Screen Protein A Acceptor Beads (PerkinElmer) and Anti-FLAG Alpha Donor Beads (PerkinElmer ...
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bioRxiv - Bioengineering 2019Quote: ... were analysed based on the Mehlich-3 extraction procedure (Mehlich, 1984) preceding inductively coupled plasma optical emission spectroscopy (ICP-OES, Optima 800, Winlab 5.5, PerkinElmer Inc.,Waltham, MA, USA). Exchangeable acidity (H + Al ...
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bioRxiv - Immunology 2021Quote: ... Proteins were subsequently blotted onto PROTRAN Pure nitrocellulose membrane (PerkinElmer) using transfer buffer (25mM TRIS ...
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bioRxiv - Microbiology 2021Quote: ... proteins were detected with WesternLightning ECL Reagent (Perkin Elmer, Germany) and exposure of the membrane to CL-XPosure film (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were revealed using Lightning® Plus ECL (Perkin Elmer) and the bands were analysed by densitometry using ImageJ (NIH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein signal was detected using enhanced chemiluminescence (ECL) reagent (PerkinElmer). The antibodies used were obtained from commercial sources ...