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Citations for PerkinElmer :
1 - 50 of 906 citations for Mouse Mitogen Activated Protein Kinase Kinase Kinase MLT MLTK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: AlphaLISA® SureFire® Ultra™ cellular kinase immunoassays were used to measure endogenous kinase activity of p-mTOR (Ser2448) (PerkinElmer, #ALSU-PMTOR-C500), p-ERK 1/2 (Thr202/Tyr204 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a given histidine kinase was first autophosphorylated with [γ-32P]-ATP (Perkin Elmer) for 90 minutes at 30 °C to drive autophosphorylation and then mixed at a 1:4 molar ratio with a response regulator (1 μM EnvZ ...
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bioRxiv - Molecular Biology 2023Quote: ... Probes were radiolabelled with 32P-ATP by poly nucleotide kinase (PNK) (PerkinElmer PNK) for 1 hour at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The level of phosphorylated extracellular signal-regulated protein kinase 1/2 (pERK1/2) was detected using the AlphaScreenTM SureFire Kit (PerkinElmer Life and Analytical Sciences). Briefly ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA probes were 5’-phosphorylated with T4 polynucleotide kinase (PNK, TOYOBO) and [γ-32P] ATP (PerkinElmer). Northern blotting of tRNAs was performed using PerfectHyb (Toyobo ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the kinase was autophosphorylated for 1 h at 30 °C with [γ-32P]ATP (Perkin Elmer) before being combined with RRs at a 1:4 ratio (10-μl reactions contained 1 μM EnvZ and 4 μM RR) ...
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bioRxiv - Molecular Biology 2020Quote: mTOR kinase activity was monitored by LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET, PerkinElmer) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The RNA 5’ phosphate was removed by quick dephosphorylation kit (New England biolabs) and phosphorylated by T4 polynucleotide kinase (New England biolabs) and [γ -32P]ATP (3000 Ci/mmol) (Perkin Elmer). The samples were loaded onto 10% polyacrylamide denaturing gel (8 M urea) ...
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bioRxiv - Physiology 2021Quote: ... immunoprecipitates were analyzed for kinase activity in presence of phosphatidylinositol (PI) and γ-32P-ATP (PerkinElmer, Santa Clara, CA) followed a published method 91 ...
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bioRxiv - Microbiology 2022Quote: ... Extracted crRNAs were end-labeled with T4 Polynucleotide Kinase in a reaction containing γ-[32P]-ATP (PerkinElmer, MA, USA), and resolved on an 8% Urea PAGE ...
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bioRxiv - Neuroscience 2021Quote: ... Then 5 µl of each diluted cell lysate was mixed with 15 µl of cell lysis buffer containing 0.33 mM ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.) in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 0.2 µM kinase was incubated with 7 µM substrate and 0.2 mM ATP containing 0.45 mCi [32P]-γ-ATP/mL (Perkin Elmer) in kinase buffer (50 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2023Quote: ... 10 mg of purified GST-fusion protein (unedited Cpx7AI125,N130 or edited Cpx7AI125M,N130S) was used per reaction and incubated with 2,500 units of recombinant kinase and [32P]ATP (Perkin Elmer). Reaction products were separated by SDS-PAGE and gels were stained with Bio-Safe Coomassie Blue (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... or 3.3 mM (Figure 5-figure supplement 1 and 8) ATP and 5 µM ProfilerPro Kinase Peptide Substrate 11 5-FAM-KKLNRTLSVA-COOH (PerkinElmer, U.S.A.), in the presence or absence of 0.66 µM CaM (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Table 9) and the compounds added to plates using the then kinase compounds added by the Sciclone ALH3000 robot (Perkin Elmer). Organoids were imaged using the Cytation5 (BioTek ...
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bioRxiv - Molecular Biology 2023Quote: ... incubation with a 32P-labelled oligodeoxyribonucleotide probe complementary to the target RNAs (32P-labelled using T4 polynucleotide kinase and [g 32P]-ATP [Perkin Elmer]) overnight in 37°C in SES1 buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Immuno-complexes were incubated for 20 min at 30°C in 30 μl of kinase buffer with a final concentration of 50 μM ATP and [γ-32P] ATP (1 x 10−2 μCi/pmol; PerkinElmer). Reactions were stopped by adding 5X LB ...
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bioRxiv - Cell Biology 2021Quote: ... Then it was mixed with reaction B which contain 5 μg of the GST-substrate in 5x Kinase buffer containing 2 μCi γ-32P-ATP (PerkinElmer, BLU002Z250UC) in 10-μl reactions ...
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bioRxiv - Neuroscience 2023Quote: ... produced in Sf9 cells, and ROCK1 (Carna Biosciences, Natick, MA; 5 nM) using the Lance-FRET kinase assay system (PerkinElmer, Waltham, MA). Other human recombinant PKCs (SignalChem Biotech ...
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bioRxiv - Biochemistry 2020Quote: ... 50 µg/mL PEG20,000 (SERVA; Cat. # 33138), ATP (Sigma; Cat. # A-7699) (variable, corresponding to ATP-Km of the respective kinase, [33P]- γ-ATP (PerkinElmer; Cat. # NEG302H): approx ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
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bioRxiv - Biochemistry 2023Quote: ... 9-1-1 having an N-terminal 7-residue kinase recognition sequence on Mec3 was radiolabeled to a specific activity of approximately 70 cpm/fmol with [γ-32P] ATP (PerkinElmer Life Sciences, Inc) using the recombinant catalytic subunit of cAMP-dependent protein kinase produced in E ...
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bioRxiv - Cell Biology 2021Quote: ... Reactions were started by adding 40 µl IR in alkyl maltoside micelles to 10 µl radioactive kinase reaction buffer (0.4 mM ATP, 1 µCi [γ-32P]-ATP (PerkinElmer, 10 mCi/mL, 25-50 cpm/mol), 1 mM MgCl2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 mM EDTA and 2-[125I]-MLT as radioligand (PerkinElmer, Waltham, MA, USA), using membranes extracted from HEK293 cells stably expressing human MT1 or MT2 receptors ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with rabbit anti-human IgG (Jackson Laboratories ...
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bioRxiv - Pathology 2020Quote: ... high protein binding ELISA plates (PerkinElmer) were coated overnight at 4°C with anti-myc antibody 9E10 at 4 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... Proteins were then transferred to methanol-activated PDVF membrane (Perkin Elmer) using transfer buffer (0.025M Tris ...
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bioRxiv - Neuroscience 2022Quote: ... The cortisol ELISA assay was performed as per manufacturer’s instructions using the Cisbio HTRF cortisol ELISA kit (62CRTPEG, Cisbio, Perkin Elmer) and measurements were performed on Tecan Infinite M1000 Pro multi-plate reader.
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bioRxiv - Molecular Biology 2019Quote: ... The DuPont HIV-1 p24 Core Profile ELISA kit (Perkin Elmer, Waltham ...
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bioRxiv - Microbiology 2022Quote: ... an equivalent of 300 ng of Gag p24 was lysed in ELISA lysis buffer containing 1% Triton (Alliance HIV-1 p24 Antigen ELISA kit, Perkin Elmer) and protease inhibitors for 30 min on ice ...
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bioRxiv - Cell Biology 2019Quote: ... Viral titer was determined using p24 ELISA test measuring viral capsid protein p24 (Perkin Elmer, performed in Biomedicum Functional Genomics Unit) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL/well) was captured overnight at 4°C onto high-protein-binding ELISA plates (Spectraplate 96HB, Perkin Elmer). Plates were washed in PBS/Tween (0.05% v/v ...
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bioRxiv - Neuroscience 2023Quote: ... and then transferred to methanol activated PVDF membranes (Perkin Elmer). Blots were blocked with 5% milk in TBS-T (0.1% Tween ...
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bioRxiv - Microbiology 2020Quote: ... MOLT-4/CCR5 cells were added on day 2 and culture supernatants were examined for the p24 viral capsid protein by ELISA (PerkinElmer) after 14 ...
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bioRxiv - Microbiology 2023Quote: ... Virus concentration was estimated by p24 titration (HIV-1 alliance p24 ELISA kit, PerkinElmer, Cat. #NEK050001KT).
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bioRxiv - Immunology 2022Quote: ... and transferred to methanol-activated PVDF membrane (Perkin Elmer, Waltham, MA) using transfer buffer (0.025M Tris ...
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bioRxiv - Microbiology 2021Quote: ... Gag p24 antigen concentration was measured with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... by measuring the Gag p24 antigen concentration with the Alliance HIV-1 p24 Antigen ELISA kit (Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: ... ELISA 384 well HB plates (Perkin Elmer), were coated with mouse recPrP23-231 at 87 nM in PBS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and ELAST ELISA Amplification System (PerkinElmer, USA) was also employed.
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bioRxiv - Microbiology 2021Quote: ... an equivalent of 500 ng of p24 Gag was lysed in buffer with 1% Triton (ELISA kit, Alliance Perkin Elmer) for 30 min on ice ...
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bioRxiv - Pathology 2023Quote: ... the p24 core antigen was detected directly in the viral supernatant with a HIV-1 p24 ELISA kit (Perkin Elmer), as specified by the supplier ...
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bioRxiv - Cancer Biology 2023Quote: ... the luminescence of activated MBD2-luciferase was measured using a MicroBeta Trilux (Perkin Elmer) microplate reader ...
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bioRxiv - Neuroscience 2020Quote: ... Protein signals were developed by using ECL kit (NEL103001EA, Perkin Elmer). Quantification of interested protein bands were conducted by using NIH Image J software ...
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bioRxiv - Microbiology 2020Quote: ... The concentration of HIV-1 Gag p24 antigen in supernatants was determined with HIV-1 p24 ELISA kit (PerkinElmer, Inc. Waltham, MA). To analyze HIV-1 Env expression ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 p24 content (produced by the backbone vector) was quantified using the Alliance p24 ELISA Kit (PerkinElmer Health Sciences, Cat# NEK050B001KT). Relative luminescence units (RLUs ...
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bioRxiv - Cancer Biology 2019Quote: ... Fluorescent signals were recorded by an ELISA reader (PerkinElmer, USA).
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bioRxiv - Microbiology 2020Quote: ... All virus supernatant was quantified via p24 ELISA (Perkin Elmer).
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bioRxiv - Biochemistry 2020Quote: ... proteins were visualized using the Western Lightning ECL II Kit (Perkin Elmer) and exposed on X-Ray film (GE Healthcare Amersham Hyperfilm ECL ...
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bioRxiv - Pathology 2022Quote: ... or Immpress anti-mouse/rabbit coupled with tyramide amplification kit (PerkinElmer, Inc). Nuclei were counterstained with DAPI.