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Citations for PerkinElmer :
1 - 50 of 59 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Purified hDGAT1 (with his tag) was absorbed onto Copper HIS-Tag PVT beads (Perkin Elmer, RPNQ0095) and incubated with [3H]-Acetyl-CoA (ARC ...
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bioRxiv - Neuroscience 2020Quote: ... using Copper HIS-Tag YSi SPA beads (Perkin Elmer) and radioactively labeled Organon ([3H]Org24598 ...
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bioRxiv - Cancer Biology 2020Quote: ... YSi (Yttrium silicate) copper-coated His-tag beads (Perkin Elmer) were used in combination with α[32P]GTP (American Radiolabeled Chemicals ...
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bioRxiv - Biophysics 2023Quote: ... LeuT was mixed with Yttrium Silicate Copper (YSi-Cu) His-tag SPA beads (PerkinElmer) and [3H]leucine in binding buffer (200 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: Binding of nucleobases was performed by means of the scintillation proximity assay (SPA) using His-tagged PurTCp in conjunction with Copper HIS-Tag YSI beads (Perkin Elmer, RPNQ0096). 250 ng of purified PurTCp were immobilized on 125 μg SPA beads per 100-μL assay in 200 mM Tris/Mes at the indicated pH ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.5/0-800 mM NaCl (equimolar substitution of NaCl with Tris/Mes)/20% glycerol/0.1 mM TCEP/0.1% DDM using 1.25 mg/mL copper His-tag PVT SPA beads (Perkin Elmer). Total binding was corrected for the non-proximity-based signal to determine the specific binding activity of each LeuT variant and data were normalized to the maximal binding observed for LeuT-WT that was assayed in parallel in all individual measurements ...
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bioRxiv - Biophysics 2019Quote: AlphaScreen-based peptide recruitments assays were performed using streptavidin coated donor beads and anti-His-tag antibody coated AlphaLisa acceptor beads (Perkin Elmer). The assays were very sensitive to the distance of the N-terminus to the LXXLL NR-box motif and the use of a flexible Ahx linker ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were tested for their ability to disrupt a second Alphascreen assay in which donor and acceptor beads were reacted with 50 nM concentrations of a peptide consisting of a biotinylation site and a His-tag peptide (PerkinElmer Life Sciences).
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bioRxiv - Bioengineering 2024Quote: ... Vivo-Tag 680 XL (PerkinElmer #NEV11119) was used as a fluorescent dye to track idLFA1 and control murine serum albumin (MSA ...
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bioRxiv - Cancer Biology 2023Quote: ... Europium labelled anti-His (Perkin Elmer # AD0110) and XL665 labelled streptavidin (CIS Bio 610SAXAC ...
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bioRxiv - Neuroscience 2021Quote: ... oil objective on a NikonTi-E stand (PerkinElmer) with a Hamamatsu C9100–50 camera ...
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bioRxiv - Microbiology 2019Quote: ... Anti-His Donor and Streptavidin Acceptor AlphaLISA beads (PerkinElmer) were added at final concentration 10 mg/mL to each well and incubated on RT for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 nM Europium labeled anti-His antibody (Perkin Elmer), 0.5x Streptavidin-xl665 (Cis Bio ...
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bioRxiv - Cell Biology 2019Quote: ... Easy-Tag [35S]-Cys/Met was purchased from PerkinElmer Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... Chemical structure of E- 4031 was drawn using ChemDraw (PerkinElmer) and converted to a 3D structure using OpenBabel (O’Boyle et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... with the DNA 1K / 12K / Hi Sensitivity Assay LabChip (Perkin Elmer) and the LabChip DNA High Sensitivity Reagent Kit ...
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bioRxiv - Microbiology 2021Quote: ... 10 µL of anti-6X His acceptor beads (Perkin Elmer, reference AL178) were then added and after 1h of incubation at RT with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... The 5 μg of thawed S1 protein was radioactively labeled with 1 mCi 125I (Perkin Elmer, Waltman, MA) using the chloramine-T method 30 ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibody to adiponectin (Table S1) was visualized with Western Lightning Plus (Perkin Elmer, Waltham, Massachusetts) and imaged using a LI-COR Odyssey Imager (LI-COR Biosciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... S1 were extracted from bioluminescence imaging data captured with an IVIS Lumina Series III (Perkin Elmer, Waltham, MA) and analyzed with Living Image 4.5.2 ...
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bioRxiv - Cell Biology 2021Quote: ... and 100μl Easy Tag Express 35S Methionine/Cysteine (Perkin Elmer NEG 772002MC) at either 0 or 24h after transformation with a 6xMyc-Pex14 (pAMW-Pex14 ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-coated Alpha donor beads or anti-His-conjugated AlphaLISA acceptor beads (PerkinElmer) were used at a final concentration of 10 μg/mL per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 130 of the colonies were analysed by colony PCR (using an M13 primer set, Table S1) and capillary electrophoresis (LabChip GX, PerkinElmer) using the DNA 1K Reagent Kit with DNA HT 5K LabChip single sipper chip ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg/mL acceptor (nickel chelate) and donor (anti-his europium; both Perkin Elmer). All components were diluted to working concentrations in alphaLISA buffer (50 mM HEPES ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike protein antigen (2.0µg/mL) was directly adsorbed on half-area 96-well OptiPlate (96W HB, Perkin Elmer) microplates for 2h at 37°C in a humidified incubator.
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bioRxiv - Biochemistry 2021Quote: ... coli TrmD (10 μM) and [3H]-AdoMet (Perkin Elmer, 4200 DPM/pmol) at a final concentration of 15 μM in a buffer containing 100 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Genetics 2022Quote: ... Probe for southern blot was amplified with primers listed in table S1 and then labeled using [α-32P] 2’-deoxycytidine 5’-triphosphate (dCTP) (PerkinElmer) with Klenow fragment (3’-5’ exo- ...
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bioRxiv - Biochemistry 2024Quote: ... 250 nM purified MBP-Naa80, 200 µM substrate peptide (Figure 1 and Supplemental Table S1, 50 µM 14C-Ac-CoA (Perkin Elmer) were mixed in acetylation buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... Two probes were amplified from a plasmid template targeting the pBac arms (see Table S1 for primers) and labelled with CTP α-32P 3000Ci/mmol (Perkin Elmer) using Klenow Enzyme (NEB ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... (Figure S1) Its chemical structure was confirmed by 1H-NMR (Mercury 400, Varian, Palo Alto) and FTIR (Spectrum One, Perkin Elmer, Waltham) shown in Figure S2.
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bioRxiv - Cell Biology 2021Quote: ... Imaging was done with temperature-controlled Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1 μg of plasmid DNA and 20 μCi of Easy-tag 35S-methionine (Perkin Elmer) were mixed with a final volume of 50 μl ...
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bioRxiv - Biochemistry 2020Quote: ... coli tRNAfMet (Chemical Block) was aminoacylated with [35S]-N-formyl-L-methionine (Perkin Elmer) as described (Lancaster and Noller ...
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bioRxiv - Molecular Biology 2021Quote: ... coli tRNA nucleotidyltransferase and [α-32P] ATP (final activity 2 µCi/µL) (PerkinElmer, # BLU003X250UC) in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... coli Keio ΔsbmA used from Keio knockout collection (Horizon, a PerkinElmer Company; https://horizondiscovery.com). Wildtype E ...
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bioRxiv - Cell Biology 2023Quote: Pulse-chase experiments using 9.8 Mbq per dish of EASY-TAG EXPRESS Protein labeling mix [35S] (PerkinElmer) and subsequent immunoprecipitation using suitable antibodies and protein G or A-coupled Sepharose beads (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... coli PPase and dGTP solution (10μL/well) were added using a FlexDrop IV (Perkin Elmer) to assay plates already containing 10nL of compound solutions ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Cell Biology 2021Quote: All widefield images were captured on an inverted Nikon TE2000-E widefield microscope with Volocity Acquisition software (PerkinElmer). These objective lenses were used ...
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bioRxiv - Biophysics 2023Quote: ... CFTR variants were pulse-labeled with 0.1-0.2 mCi/ml 35S-methionine and 35S-cysteine (Easy Tag Express, Perkin Elmer) in Cys- and Met-free DMEM (Gibco™ 21013024 ...
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bioRxiv - Immunology 2022Quote: ... The quality and average fragment size of the final libraries were determined using DNA 1K/12K/Hi Sensitivity Assay LabChip and DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries with 16 unique dual indexes were pooled and sequenced on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... The average fragment size of the final libraries was determined using DNA 1K/12K/Hi Sensitivity Assay LabChip (760517, PerkinElmer)) and DNA High Sensitivity Reagent Kit (CLS760672 ...
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bioRxiv - Immunology 2021Quote: ... of the abdominal region was measured (exposure=1 sec., F/stop= 1.2, FOV=E, binning=medium, emissions filter=open) using Living Image software (PerkinElmer). Survival of the mice was recorded until 20 days after infection.
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bioRxiv - Cell Biology 2021Quote: ... Worms were imaged on a 2% agarose pad on a Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...
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bioRxiv - Molecular Biology 2023Quote: ... Imaging was performed on a Nikon ECLIPSE Ti-E-inverted microscope equipped with a spinning disk device (Perkin Elmer Ultraview ...
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bioRxiv - Cell Biology 2021Quote: ... on the Nikon TE2000-E microscope equipped with an ORCA-ER CCD camera (Hammamatsu Photonics) operated by Volocity Acquisition module software (Improvision, PerkinElmer). Collagen degradation was quantified using Fiji by measuring the integrated density of the collagen layer ...
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bioRxiv - Molecular Biology 2020Quote: ... Imaging was done on a Nikon ECLIPSE Ti-E-inverted microscope equipped with a spinning disk device (Perkin Elmer Ultraview), and images were processed using Volocity software (version 6.2.1 ...
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bioRxiv - Neuroscience 2021Quote: ... we used a spinning disc confocal microscope (Nikon Ti-E Eclipse inverted scope equipped with 488 and 561 laser lines, a Prior NZ250 Piezo stage, a PerkinElmer UltraVIEW VoX ...
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bioRxiv - Cancer Biology 2021Quote: Quantitation of each individual tumor was measured from H&E sections using morphometric analysis in Panoramic viewer software (Perkin Elmer), which calculates the size of each identified tumor by area in squared microns ...
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bioRxiv - Cell Biology 2021Quote: ... worms were immobilized with imidazole and imaged on a temperature-controlled Nikon Ti-E Eclipse inverted/UltraVIEW VoX (Perkin Elmer) spinning disc confocal setup ...